RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses
All synapses require fusion-competent vesicles and coordinated Ca2+-secretion coupling for neurotransmission, yet functional and anatomical properties are diverse across different synapse types. We show that the presynaptic protein RIM-BP2 has diversified functions in neurotransmitter release at dif...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2019-09-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/43243 |
| _version_ | 1811181125948669952 |
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| author | Marisa M Brockmann Marta Maglione Claudia G Willmes Alexander Stumpf Boris A Bouazza Laura M Velasquez M Katharina Grauel Prateep Beed Martin Lehmann Niclas Gimber Jan Schmoranzer Stephan J Sigrist Christian Rosenmund Dietmar Schmitz |
| author_facet | Marisa M Brockmann Marta Maglione Claudia G Willmes Alexander Stumpf Boris A Bouazza Laura M Velasquez M Katharina Grauel Prateep Beed Martin Lehmann Niclas Gimber Jan Schmoranzer Stephan J Sigrist Christian Rosenmund Dietmar Schmitz |
| author_sort | Marisa M Brockmann |
| collection | DOAJ |
| description | All synapses require fusion-competent vesicles and coordinated Ca2+-secretion coupling for neurotransmission, yet functional and anatomical properties are diverse across different synapse types. We show that the presynaptic protein RIM-BP2 has diversified functions in neurotransmitter release at different central murine synapses and thus contributes to synaptic diversity. At hippocampal pyramidal CA3-CA1 synapses, RIM-BP2 loss has a mild effect on neurotransmitter release, by only regulating Ca2+-secretion coupling. However, at hippocampal mossy fiber synapses, RIM-BP2 has a substantial impact on neurotransmitter release by promoting vesicle docking/priming and vesicular release probability via stabilization of Munc13-1 at the active zone. We suggest that differences in the active zone organization may dictate the role a protein plays in synaptic transmission and that differences in active zone architecture is a major determinant factor in the functional diversity of synapses. |
| first_indexed | 2024-04-11T09:14:25Z |
| format | Article |
| id | doaj.art-ebd13371dad64488b7c36a53f858e406 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:14:25Z |
| publishDate | 2019-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-ebd13371dad64488b7c36a53f858e4062022-12-22T04:32:25ZengeLife Sciences Publications LtdeLife2050-084X2019-09-01810.7554/eLife.43243RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapsesMarisa M Brockmann0https://orcid.org/0000-0002-1386-5359Marta Maglione1Claudia G Willmes2Alexander Stumpf3Boris A Bouazza4Laura M Velasquez5M Katharina Grauel6https://orcid.org/0000-0003-3542-0606Prateep Beed7Martin Lehmann8Niclas Gimber9Jan Schmoranzer10Stephan J Sigrist11https://orcid.org/0000-0002-1698-5815Christian Rosenmund12https://orcid.org/0000-0002-3905-2444Dietmar Schmitz13Institut für Neurophysiologie, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, GermanyFreie Universität Berlin, Institut für Biologie, Berlin, Germany; Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany; NeuroCure Cluster of Excellence, Berlin, GermanyDZNE, German Center for Neurodegenerative Diseases, Berlin, GermanyNeuroscience Research Center, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, GermanyInstitut für Neurophysiologie, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, GermanyNeuroscience Research Center, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, GermanyInstitut für Neurophysiologie, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, GermanyNeuroscience Research Center, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, GermanyLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, GermanyNeuroscience Research Center, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, GermanyNeuroCure Cluster of Excellence, Berlin, GermanyFreie Universität Berlin, Institut für Biologie, Berlin, Germany; NeuroCure Cluster of Excellence, Berlin, Germany; DZNE, German Center for Neurodegenerative Diseases, Berlin, GermanyInstitut für Neurophysiologie, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany; NeuroCure Cluster of Excellence, Berlin, GermanyNeuroCure Cluster of Excellence, Berlin, Germany; DZNE, German Center for Neurodegenerative Diseases, Berlin, Germany; Neuroscience Research Center, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, GermanyAll synapses require fusion-competent vesicles and coordinated Ca2+-secretion coupling for neurotransmission, yet functional and anatomical properties are diverse across different synapse types. We show that the presynaptic protein RIM-BP2 has diversified functions in neurotransmitter release at different central murine synapses and thus contributes to synaptic diversity. At hippocampal pyramidal CA3-CA1 synapses, RIM-BP2 loss has a mild effect on neurotransmitter release, by only regulating Ca2+-secretion coupling. However, at hippocampal mossy fiber synapses, RIM-BP2 has a substantial impact on neurotransmitter release by promoting vesicle docking/priming and vesicular release probability via stabilization of Munc13-1 at the active zone. We suggest that differences in the active zone organization may dictate the role a protein plays in synaptic transmission and that differences in active zone architecture is a major determinant factor in the functional diversity of synapses.https://elifesciences.org/articles/43243neurotransmitter releaseactive zonevesicle primingcalcium channelhippocampus |
| spellingShingle | Marisa M Brockmann Marta Maglione Claudia G Willmes Alexander Stumpf Boris A Bouazza Laura M Velasquez M Katharina Grauel Prateep Beed Martin Lehmann Niclas Gimber Jan Schmoranzer Stephan J Sigrist Christian Rosenmund Dietmar Schmitz RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses eLife neurotransmitter release active zone vesicle priming calcium channel hippocampus |
| title | RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses |
| title_full | RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses |
| title_fullStr | RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses |
| title_full_unstemmed | RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses |
| title_short | RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses |
| title_sort | rim bp2 primes synaptic vesicles via recruitment of munc13 1 at hippocampal mossy fiber synapses |
| topic | neurotransmitter release active zone vesicle priming calcium channel hippocampus |
| url | https://elifesciences.org/articles/43243 |
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