Enzyme Histochemical Localization of Peroxidase Activity in the Oviduct of Immature, Laying and Non-laying Hens
Peroxidase may play roles in innate host immunity and affect the sperm survivability in hen oviduct. The aim of this study was to localize the peroxidase activity in the oviduct of hens and to examine whether the activity is affected by the developmental stages of the oviduct. The oviducts were co...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2006-11-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/43/4/43_4_401/_pdf/-char/en |
| _version_ | 1797816290327396352 |
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| author | Yukinori Yoshimura Mayuko Mizuguchi Mariko Fujii Naoki Isobe |
| author_facet | Yukinori Yoshimura Mayuko Mizuguchi Mariko Fujii Naoki Isobe |
| author_sort | Yukinori Yoshimura |
| collection | DOAJ |
| description | Peroxidase may play roles in innate host immunity and affect the sperm survivability in hen oviduct. The aim of this study was to localize the peroxidase activity in the oviduct of hens and to examine whether the activity is affected by the developmental stages of the oviduct. The oviducts were collected from immature, laying and non-laying White Leghorn hens. Formalin-fixed oviductal tissues were processed for frozen sections and incubated with reaction mixture containing diaminobenzidine and H2O2. No peroxidase activity products were found in the surface epithelium of the infundibulum, magnum, uterus and uetero-vaginal junction (UVJ), in the tubular glands of magnum, isthmus and uterus, and in the sperm storage tubules in the UVJ. The activity was occasionally observed in the surface epithelial cells in the isthmus. The surface epithelial cells of the vagina contained dense peroxidase reaction products, which were distributed at the bottom region of the secondary folds. The density of reaction products and their distribution were reduced in non-laying hens, and the oviducts of immature hens contained no peroxidase reaction products. These results suggest that the surface epithelium of the vagina contains peroxidase activity in laying hens, which may play roles in innate host immunity and affect the sperm viability passing the vagina. The vaginal peroxidase activity is likely to be changed in association with the developmental stage of the oviduct. |
| first_indexed | 2024-03-13T08:35:34Z |
| format | Article |
| id | doaj.art-ebd1ae6955bc4013992af5ca3987004e |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:35:34Z |
| publishDate | 2006-11-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-ebd1ae6955bc4013992af5ca3987004e2023-05-31T00:21:10ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862006-11-0143440140710.2141/jpsa.43.401jpsaEnzyme Histochemical Localization of Peroxidase Activity in the Oviduct of Immature, Laying and Non-laying HensYukinori Yoshimura0Mayuko Mizuguchi1Mariko Fujii2Naoki Isobe3Graduate School of Biosphere Science, Hiroshima UniversityGraduate School of Biosphere Science, Hiroshima UniversityGraduate School of Biosphere Science, Hiroshima UniversityGraduate School of Biosphere Science, Hiroshima UniversityPeroxidase may play roles in innate host immunity and affect the sperm survivability in hen oviduct. The aim of this study was to localize the peroxidase activity in the oviduct of hens and to examine whether the activity is affected by the developmental stages of the oviduct. The oviducts were collected from immature, laying and non-laying White Leghorn hens. Formalin-fixed oviductal tissues were processed for frozen sections and incubated with reaction mixture containing diaminobenzidine and H2O2. No peroxidase activity products were found in the surface epithelium of the infundibulum, magnum, uterus and uetero-vaginal junction (UVJ), in the tubular glands of magnum, isthmus and uterus, and in the sperm storage tubules in the UVJ. The activity was occasionally observed in the surface epithelial cells in the isthmus. The surface epithelial cells of the vagina contained dense peroxidase reaction products, which were distributed at the bottom region of the secondary folds. The density of reaction products and their distribution were reduced in non-laying hens, and the oviducts of immature hens contained no peroxidase reaction products. These results suggest that the surface epithelium of the vagina contains peroxidase activity in laying hens, which may play roles in innate host immunity and affect the sperm viability passing the vagina. The vaginal peroxidase activity is likely to be changed in association with the developmental stage of the oviduct.https://www.jstage.jst.go.jp/article/jpsa/43/4/43_4_401/_pdf/-char/enenzyme histochemistryhenoviductperoxidasevagina |
| spellingShingle | Yukinori Yoshimura Mayuko Mizuguchi Mariko Fujii Naoki Isobe Enzyme Histochemical Localization of Peroxidase Activity in the Oviduct of Immature, Laying and Non-laying Hens The Journal of Poultry Science enzyme histochemistry hen oviduct peroxidase vagina |
| title | Enzyme Histochemical Localization of Peroxidase Activity in the Oviduct of Immature, Laying and Non-laying Hens |
| title_full | Enzyme Histochemical Localization of Peroxidase Activity in the Oviduct of Immature, Laying and Non-laying Hens |
| title_fullStr | Enzyme Histochemical Localization of Peroxidase Activity in the Oviduct of Immature, Laying and Non-laying Hens |
| title_full_unstemmed | Enzyme Histochemical Localization of Peroxidase Activity in the Oviduct of Immature, Laying and Non-laying Hens |
| title_short | Enzyme Histochemical Localization of Peroxidase Activity in the Oviduct of Immature, Laying and Non-laying Hens |
| title_sort | enzyme histochemical localization of peroxidase activity in the oviduct of immature laying and non laying hens |
| topic | enzyme histochemistry hen oviduct peroxidase vagina |
| url | https://www.jstage.jst.go.jp/article/jpsa/43/4/43_4_401/_pdf/-char/en |
| work_keys_str_mv | AT yukinoriyoshimura enzymehistochemicallocalizationofperoxidaseactivityintheoviductofimmaturelayingandnonlayinghens AT mayukomizuguchi enzymehistochemicallocalizationofperoxidaseactivityintheoviductofimmaturelayingandnonlayinghens AT marikofujii enzymehistochemicallocalizationofperoxidaseactivityintheoviductofimmaturelayingandnonlayinghens AT naokiisobe enzymehistochemicallocalizationofperoxidaseactivityintheoviductofimmaturelayingandnonlayinghens |