Structure-guided manipulation of the regioselectivity of the cyclosporine A hydroxylase CYP-sb21 from Sebekia benihana

Structure-guided manipulation of the regioselectivity of the cyclosporine A hydroxylase CYP-sb21 from Sebekia benihana

The cytochrome P450 enzyme CYP-sb21 from the rare actinomycete Sebekia benihana is capable of hydroxylating the immunosuppressive drug molecule cyclosporine A (CsA) primarily at the 4th N-methyl leucine (MeLeu4), giving rise to γ-hydroxy-N-methyl-l-Leu4-CsA (CsA-4-OH). This oxidative modification of...

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Bibliographic Details
Main Authors: Fengwei Li, Li Ma, Xingwang Zhang, Jingfei Chen, Feifei Qi, Yinyue Huang, Zepeng Qu, Lishan Yao, Wei Zhang, Eung-Soo Kim, Shengying Li
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2020-09-01
Series:Synthetic and Systems Biotechnology
Subjects:
Cytochrome P450 monooxygenase
Crystal structure
Cyclosporine A
Regioselectivity
Hair growth stimulator
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X20300491

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