Effect of dietary mannanoligosaccharide supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogs
Abstract Background Use of prebiotics in companion animal nutrition is often considered advantageous over probiotics because of the ease of handling, ability to withstand processing and storage etc. While most of the studies on prebiotic use in dogs have been done with processed food as basal diet,...
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| Format: | Article |
| Language: | English |
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Korean Society of Animal Sciences and Technology
2017-05-01
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| Series: | Journal of Animal Science and Technology |
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| Online Access: | http://link.springer.com/article/10.1186/s40781-017-0136-6 |
| _version_ | 1818475221717352448 |
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| author | Mahesh M. Pawar Ashok K. Pattanaik Dharmendra K. Sinha Tapas K. Goswami Kusumakar Sharma |
| author_facet | Mahesh M. Pawar Ashok K. Pattanaik Dharmendra K. Sinha Tapas K. Goswami Kusumakar Sharma |
| author_sort | Mahesh M. Pawar |
| collection | DOAJ |
| description | Abstract Background Use of prebiotics in companion animal nutrition is often considered advantageous over probiotics because of the ease of handling, ability to withstand processing and storage etc. While most of the studies on prebiotic use in dogs have been done with processed food as basal diet, the response in relation to homemade diet feeding is not very well explored. Methods The study was conducted to evaluate the effects of dietary mannanoligosaccharide (MOS) supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogs. Ten Spitz pups were divided into two groups: control (CON) with no supplementation, and experimental (MOS) wherein the basal diet was supplemented with MOS at 15 g/kg diet. All dogs were fed on a home-prepared diet for a period of 150 days. The study protocol included a digestion trial, periodic blood collection and analysis for lipid profile and erythrocytic antioxidants. Immune response of the animals was assessed towards the end of the feeding period. Results Results revealed no significant (P > 0.05) variations in palatability score, intake and apparent digestibility of nutrients between the groups. Faecal score, faeces voided, faecal pH, concentrations of ammonia, lactate and short-chain fatty acids were comparable (P > 0.05) between the two groups. Cell-mediated immune response, assessed as delayed-type of hypersensitivity response, was significantly higher (P < 0.05) in the MOS group. The percent of lymphocyte sub-populations CD4+ and ratio of CD4+:CD8+ were also significantly (P < 0.05) higher in MOS group. The serum IgG levels were similar (P > 0.05) in both the groups. Supplementation of MOS lowered (P < 0.05) serum total- and LDL- cholesterol levels, when compared with the control group. The erythrocytic antioxidant indices were similar (P > 0.05) between the two groups. Conclusions The results indicated that supplementation of MOS at the rate of 15 g/kg in the diet of dog augmented the cell-mediated immune response and serum lipid profile without any influences on digestibility of nutrients, hindgut fermentation and antioxidants indices. |
| first_indexed | 2024-12-10T09:10:13Z |
| format | Article |
| id | doaj.art-ebd6fd5d0d254495ba519c38f743bdb0 |
| institution | Directory Open Access Journal |
| issn | 2055-0391 |
| language | English |
| last_indexed | 2024-12-10T09:10:13Z |
| publishDate | 2017-05-01 |
| publisher | Korean Society of Animal Sciences and Technology |
| record_format | Article |
| series | Journal of Animal Science and Technology |
| spelling | doaj.art-ebd6fd5d0d254495ba519c38f743bdb02022-12-22T01:55:02ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2055-03912017-05-015911710.1186/s40781-017-0136-6Effect of dietary mannanoligosaccharide supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogsMahesh M. Pawar0Ashok K. Pattanaik1Dharmendra K. Sinha2Tapas K. Goswami3Kusumakar Sharma4Division of Animal Nutrition, ICAR-Indian Veterinary Research InstituteDivision of Animal Nutrition, ICAR-Indian Veterinary Research InstituteDivision of Epidemiology, ICAR-Indian Veterinary Research InstituteImmunology Section, ICAR-Indian Veterinary Research InstituteDivision of Animal Nutrition, ICAR-Indian Veterinary Research InstituteAbstract Background Use of prebiotics in companion animal nutrition is often considered advantageous over probiotics because of the ease of handling, ability to withstand processing and storage etc. While most of the studies on prebiotic use in dogs have been done with processed food as basal diet, the response in relation to homemade diet feeding is not very well explored. Methods The study was conducted to evaluate the effects of dietary mannanoligosaccharide (MOS) supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogs. Ten Spitz pups were divided into two groups: control (CON) with no supplementation, and experimental (MOS) wherein the basal diet was supplemented with MOS at 15 g/kg diet. All dogs were fed on a home-prepared diet for a period of 150 days. The study protocol included a digestion trial, periodic blood collection and analysis for lipid profile and erythrocytic antioxidants. Immune response of the animals was assessed towards the end of the feeding period. Results Results revealed no significant (P > 0.05) variations in palatability score, intake and apparent digestibility of nutrients between the groups. Faecal score, faeces voided, faecal pH, concentrations of ammonia, lactate and short-chain fatty acids were comparable (P > 0.05) between the two groups. Cell-mediated immune response, assessed as delayed-type of hypersensitivity response, was significantly higher (P < 0.05) in the MOS group. The percent of lymphocyte sub-populations CD4+ and ratio of CD4+:CD8+ were also significantly (P < 0.05) higher in MOS group. The serum IgG levels were similar (P > 0.05) in both the groups. Supplementation of MOS lowered (P < 0.05) serum total- and LDL- cholesterol levels, when compared with the control group. The erythrocytic antioxidant indices were similar (P > 0.05) between the two groups. Conclusions The results indicated that supplementation of MOS at the rate of 15 g/kg in the diet of dog augmented the cell-mediated immune response and serum lipid profile without any influences on digestibility of nutrients, hindgut fermentation and antioxidants indices.http://link.springer.com/article/10.1186/s40781-017-0136-6AntioxidantsDogsHindgut fermentationImmunityMannanoligosaccharide |
| spellingShingle | Mahesh M. Pawar Ashok K. Pattanaik Dharmendra K. Sinha Tapas K. Goswami Kusumakar Sharma Effect of dietary mannanoligosaccharide supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogs Journal of Animal Science and Technology Antioxidants Dogs Hindgut fermentation Immunity Mannanoligosaccharide |
| title | Effect of dietary mannanoligosaccharide supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogs |
| title_full | Effect of dietary mannanoligosaccharide supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogs |
| title_fullStr | Effect of dietary mannanoligosaccharide supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogs |
| title_full_unstemmed | Effect of dietary mannanoligosaccharide supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogs |
| title_short | Effect of dietary mannanoligosaccharide supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogs |
| title_sort | effect of dietary mannanoligosaccharide supplementation on nutrient digestibility hindgut fermentation immune response and antioxidant indices in dogs |
| topic | Antioxidants Dogs Hindgut fermentation Immunity Mannanoligosaccharide |
| url | http://link.springer.com/article/10.1186/s40781-017-0136-6 |
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