Genetic Basis of Natural Variation in Spontaneous Grooming in Drosophila melanogaster
Spontaneous grooming behavior is a component of insect fitness. We quantified spontaneous grooming behavior in 201 sequenced lines of the Drosophila melanogaster Genetic Reference Panel and observed significant genetic variation in spontaneous grooming, with broad-sense heritabilities of 0.25 and 0....
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| Format: | Article |
| Language: | English |
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Oxford University Press
2020-09-01
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| Series: | G3: Genes, Genomes, Genetics |
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| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.120.401360 |
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| author | Aya Yanagawa Wen Huang Akihiko Yamamoto Ayako Wada-Katsumata Coby Schal Trudy F. C. Mackay |
| author_facet | Aya Yanagawa Wen Huang Akihiko Yamamoto Ayako Wada-Katsumata Coby Schal Trudy F. C. Mackay |
| author_sort | Aya Yanagawa |
| collection | DOAJ |
| description | Spontaneous grooming behavior is a component of insect fitness. We quantified spontaneous grooming behavior in 201 sequenced lines of the Drosophila melanogaster Genetic Reference Panel and observed significant genetic variation in spontaneous grooming, with broad-sense heritabilities of 0.25 and 0.24 in females and males, respectively. Although grooming behavior is highly correlated between males and females, we observed significant sex by genotype interactions, indicating that the genetic basis of spontaneous grooming is partially distinct in the two sexes. We performed genome-wide association analyses of grooming behavior, and mapped 107 molecular polymorphisms associated with spontaneous grooming behavior, of which 73 were in or near 70 genes and 34 were over 1 kilobase from the nearest gene. The candidate genes were associated with a wide variety of gene ontology terms, and several of the candidate genes were significantly enriched in a genetic interaction network. We performed functional assessments of 29 candidate genes using RNA interference, and found that 11 affected spontaneous grooming behavior. The genes associated with natural variation in Drosophila grooming are involved with glutamate metabolism (Gdh) and transport (Eaat); interact genetically with (CCKLR-17D1) or are in the same gene family as (PGRP-LA) genes previously implicated in grooming behavior; are involved in the development of the nervous system and other tissues; or regulate the Notch and Epidermal growth factor receptor signaling pathways. Several DGRP lines exhibited extreme grooming behavior. Excessive grooming behavior can serve as a model for repetitive behaviors diagnostic of several human neuropsychiatric diseases. |
| first_indexed | 2024-12-14T17:30:53Z |
| format | Article |
| id | doaj.art-ebd96766bd78444e97fc5f5dbcffd3a3 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-14T17:30:53Z |
| publishDate | 2020-09-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-ebd96766bd78444e97fc5f5dbcffd3a32022-12-21T22:53:06ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362020-09-011093453346010.1534/g3.120.40136048Genetic Basis of Natural Variation in Spontaneous Grooming in Drosophila melanogasterAya YanagawaWen HuangAkihiko YamamotoAyako Wada-KatsumataCoby SchalTrudy F. C. MackaySpontaneous grooming behavior is a component of insect fitness. We quantified spontaneous grooming behavior in 201 sequenced lines of the Drosophila melanogaster Genetic Reference Panel and observed significant genetic variation in spontaneous grooming, with broad-sense heritabilities of 0.25 and 0.24 in females and males, respectively. Although grooming behavior is highly correlated between males and females, we observed significant sex by genotype interactions, indicating that the genetic basis of spontaneous grooming is partially distinct in the two sexes. We performed genome-wide association analyses of grooming behavior, and mapped 107 molecular polymorphisms associated with spontaneous grooming behavior, of which 73 were in or near 70 genes and 34 were over 1 kilobase from the nearest gene. The candidate genes were associated with a wide variety of gene ontology terms, and several of the candidate genes were significantly enriched in a genetic interaction network. We performed functional assessments of 29 candidate genes using RNA interference, and found that 11 affected spontaneous grooming behavior. The genes associated with natural variation in Drosophila grooming are involved with glutamate metabolism (Gdh) and transport (Eaat); interact genetically with (CCKLR-17D1) or are in the same gene family as (PGRP-LA) genes previously implicated in grooming behavior; are involved in the development of the nervous system and other tissues; or regulate the Notch and Epidermal growth factor receptor signaling pathways. Several DGRP lines exhibited extreme grooming behavior. Excessive grooming behavior can serve as a model for repetitive behaviors diagnostic of several human neuropsychiatric diseases.http://g3journal.org/lookup/doi/10.1534/g3.120.401360drosophila genetic reference panelgenome wide association analysisrna interferencebehavioral genetics |
| spellingShingle | Aya Yanagawa Wen Huang Akihiko Yamamoto Ayako Wada-Katsumata Coby Schal Trudy F. C. Mackay Genetic Basis of Natural Variation in Spontaneous Grooming in Drosophila melanogaster G3: Genes, Genomes, Genetics drosophila genetic reference panel genome wide association analysis rna interference behavioral genetics |
| title | Genetic Basis of Natural Variation in Spontaneous Grooming in Drosophila melanogaster |
| title_full | Genetic Basis of Natural Variation in Spontaneous Grooming in Drosophila melanogaster |
| title_fullStr | Genetic Basis of Natural Variation in Spontaneous Grooming in Drosophila melanogaster |
| title_full_unstemmed | Genetic Basis of Natural Variation in Spontaneous Grooming in Drosophila melanogaster |
| title_short | Genetic Basis of Natural Variation in Spontaneous Grooming in Drosophila melanogaster |
| title_sort | genetic basis of natural variation in spontaneous grooming in drosophila melanogaster |
| topic | drosophila genetic reference panel genome wide association analysis rna interference behavioral genetics |
| url | http://g3journal.org/lookup/doi/10.1534/g3.120.401360 |
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