Structure Insights Into Photosystem I Octamer From Cyanobacteria
The diversity of photosystem oligomers is essential to understanding how photosynthetic organisms adapt to light conditions. Due to its structural and physiological significance, the assembly of the PSI supercomplex has been of great interest recently in terms of both chloroplast and cyanobacteria....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.876122/full |
| _version_ | 1818201664857833472 |
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| author | Ming Chen Yujie He Dongyang Liu Lijin Tian Pengqi Xu Xuan Liu Yihang Pan Shuqi Dong Shuqi Dong Jun He Jun He Ying Zhang |
| author_facet | Ming Chen Yujie He Dongyang Liu Lijin Tian Pengqi Xu Xuan Liu Yihang Pan Shuqi Dong Shuqi Dong Jun He Jun He Ying Zhang |
| author_sort | Ming Chen |
| collection | DOAJ |
| description | The diversity of photosystem oligomers is essential to understanding how photosynthetic organisms adapt to light conditions. Due to its structural and physiological significance, the assembly of the PSI supercomplex has been of great interest recently in terms of both chloroplast and cyanobacteria. In this study, two novel photosystem I supercomplexes were isolated for the first time from the low light incubated culture of filamentous cyanobacterium Anabaena sp. PCC 7120. These complexes were defined as PSI hexamers and octamers through biochemical and biophysical characterization. Their 77K emission spectra indicated that the red forms of chlorophylls seemed not to be affected during oligomerization. By cryo-EM single-particle analysis, a near-atomic (7.0 Å) resolution structure of a PSI octamer was resolved, and the molecular assemblies of a stable PSI octamer were revealed. |
| first_indexed | 2024-12-12T02:57:09Z |
| format | Article |
| id | doaj.art-ebdaf78052c74181a70fa9223f0e3a86 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T02:57:09Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ebdaf78052c74181a70fa9223f0e3a862022-12-22T00:40:42ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-05-011310.3389/fmicb.2022.876122876122Structure Insights Into Photosystem I Octamer From CyanobacteriaMing Chen0Yujie He1Dongyang Liu2Lijin Tian3Pengqi Xu4Xuan Liu5Yihang Pan6Shuqi Dong7Shuqi Dong8Jun He9Jun He10Ying Zhang11The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaCenter for Cell Fate and Lineage (CCLA), Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, ChinaPhotosynthesis Research Centre, Institute of Botany, Chinese Academy of Sciences (CAS), Beijing, ChinaPhotosynthesis Research Centre, Institute of Botany, Chinese Academy of Sciences (CAS), Beijing, ChinaThe Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaThe Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaThe Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaCenter for Cell Fate and Lineage (CCLA), Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, ChinaCenter for Cell Lineage and Development, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaCenter for Cell Fate and Lineage (CCLA), Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, ChinaCenter for Cell Lineage and Development, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaThe Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaThe diversity of photosystem oligomers is essential to understanding how photosynthetic organisms adapt to light conditions. Due to its structural and physiological significance, the assembly of the PSI supercomplex has been of great interest recently in terms of both chloroplast and cyanobacteria. In this study, two novel photosystem I supercomplexes were isolated for the first time from the low light incubated culture of filamentous cyanobacterium Anabaena sp. PCC 7120. These complexes were defined as PSI hexamers and octamers through biochemical and biophysical characterization. Their 77K emission spectra indicated that the red forms of chlorophylls seemed not to be affected during oligomerization. By cryo-EM single-particle analysis, a near-atomic (7.0 Å) resolution structure of a PSI octamer was resolved, and the molecular assemblies of a stable PSI octamer were revealed.https://www.frontiersin.org/articles/10.3389/fmicb.2022.876122/fullcyanobacteriaphotosystem Ihexameroctamersingle particle |
| spellingShingle | Ming Chen Yujie He Dongyang Liu Lijin Tian Pengqi Xu Xuan Liu Yihang Pan Shuqi Dong Shuqi Dong Jun He Jun He Ying Zhang Structure Insights Into Photosystem I Octamer From Cyanobacteria Frontiers in Microbiology cyanobacteria photosystem I hexamer octamer single particle |
| title | Structure Insights Into Photosystem I Octamer From Cyanobacteria |
| title_full | Structure Insights Into Photosystem I Octamer From Cyanobacteria |
| title_fullStr | Structure Insights Into Photosystem I Octamer From Cyanobacteria |
| title_full_unstemmed | Structure Insights Into Photosystem I Octamer From Cyanobacteria |
| title_short | Structure Insights Into Photosystem I Octamer From Cyanobacteria |
| title_sort | structure insights into photosystem i octamer from cyanobacteria |
| topic | cyanobacteria photosystem I hexamer octamer single particle |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.876122/full |
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