Effect of 70% alcohol swabbing and flushing technique on high speed contra angle towards bacterial count and identification of pathogens

High-speed contra angle is a potential source of cross-infection due to its working system in blowing up water, saliva and microorganisms from the patients, as well as dental unit water line contamination. This experimental Quasy laboratory study aimed to determine the reduce of bacteria in high-spe...

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Main Author: Sri Mulyanti
Format: Article
Language:English
Published: Universitas Padjadjaran 2009-07-01
Series:Padjadjaran Journal of Dentistry
Subjects:
Online Access:http://jurnal.unpad.ac.id/pjd/article/view/14090
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author Sri Mulyanti
author_facet Sri Mulyanti
author_sort Sri Mulyanti
collection DOAJ
description High-speed contra angle is a potential source of cross-infection due to its working system in blowing up water, saliva and microorganisms from the patients, as well as dental unit water line contamination. This experimental Quasy laboratory study aimed to determine the reduce of bacteria in high-speed contra angle after 70% alcohol swabbing and flushing for 30 seconds and identifying the presence of the pathogen. This study was conducted on 35 lower molar teeth with caries without abscess in patients during pulp opening with the contra-angle. Afterwards, the contra-angle was immersed in sterile aquadest and flushed. The immersed and flushed water were sampled then cultured on blood agar plates. The colony forming units were counted manually: Group I, was the control; Group II, after used on patients; and Group III, after treatment and swabbed with 70% alcohol and flushed. The data were statistically analyzed according to Anava and continued by test with least significant differences (LSD). The results showed that in bacterial identification there were pathogens. The bacteria amount was reduced after swabbing the contra-angle from 50.88±40.33 to 10.31±20.02 cfus. After flushing, the cfus decreased from 169.74±53.76 to 73.03±36.39. Based on the variant and LSD analyzes (p<0.01), CFU was significantly different among the three groups. Conclusion: 70% alcohol swabbing and flushing could reduce the bacterial amount in high-speed contra angle and there was the presence of Staphylococcus aureus and Pseudomonas aeruginosa on the usage of high-speed contra-angle.
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spelling doaj.art-ebdf0ecd5e904ac09719b2423fcdc2db2022-12-21T22:43:29ZengUniversitas PadjadjaranPadjadjaran Journal of Dentistry1979-02012549-62122009-07-0121210.24198/pjd.vol21no2.140907853Effect of 70% alcohol swabbing and flushing technique on high speed contra angle towards bacterial count and identification of pathogensSri Mulyanti0Bandung Health PolytechnicHigh-speed contra angle is a potential source of cross-infection due to its working system in blowing up water, saliva and microorganisms from the patients, as well as dental unit water line contamination. This experimental Quasy laboratory study aimed to determine the reduce of bacteria in high-speed contra angle after 70% alcohol swabbing and flushing for 30 seconds and identifying the presence of the pathogen. This study was conducted on 35 lower molar teeth with caries without abscess in patients during pulp opening with the contra-angle. Afterwards, the contra-angle was immersed in sterile aquadest and flushed. The immersed and flushed water were sampled then cultured on blood agar plates. The colony forming units were counted manually: Group I, was the control; Group II, after used on patients; and Group III, after treatment and swabbed with 70% alcohol and flushed. The data were statistically analyzed according to Anava and continued by test with least significant differences (LSD). The results showed that in bacterial identification there were pathogens. The bacteria amount was reduced after swabbing the contra-angle from 50.88±40.33 to 10.31±20.02 cfus. After flushing, the cfus decreased from 169.74±53.76 to 73.03±36.39. Based on the variant and LSD analyzes (p<0.01), CFU was significantly different among the three groups. Conclusion: 70% alcohol swabbing and flushing could reduce the bacterial amount in high-speed contra angle and there was the presence of Staphylococcus aureus and Pseudomonas aeruginosa on the usage of high-speed contra-angle.http://jurnal.unpad.ac.id/pjd/article/view/14090swabbing, flushing, 70% alcohol, high speed contra angle.
spellingShingle Sri Mulyanti
Effect of 70% alcohol swabbing and flushing technique on high speed contra angle towards bacterial count and identification of pathogens
Padjadjaran Journal of Dentistry
swabbing, flushing, 70% alcohol, high speed contra angle.
title Effect of 70% alcohol swabbing and flushing technique on high speed contra angle towards bacterial count and identification of pathogens
title_full Effect of 70% alcohol swabbing and flushing technique on high speed contra angle towards bacterial count and identification of pathogens
title_fullStr Effect of 70% alcohol swabbing and flushing technique on high speed contra angle towards bacterial count and identification of pathogens
title_full_unstemmed Effect of 70% alcohol swabbing and flushing technique on high speed contra angle towards bacterial count and identification of pathogens
title_short Effect of 70% alcohol swabbing and flushing technique on high speed contra angle towards bacterial count and identification of pathogens
title_sort effect of 70 alcohol swabbing and flushing technique on high speed contra angle towards bacterial count and identification of pathogens
topic swabbing, flushing, 70% alcohol, high speed contra angle.
url http://jurnal.unpad.ac.id/pjd/article/view/14090
work_keys_str_mv AT srimulyanti effectof70alcoholswabbingandflushingtechniqueonhighspeedcontraangletowardsbacterialcountandidentificationofpathogens