Detection and expression analysis of tet(B) in Streptococcus oralis
Tetracycline resistance can be achieved through tet genes, which code for efflux pumps, ribosomal protection proteins and inactivation enzymes. Some of these genes have only been described in either Gram-positive or Gram-negative bacteria. This is the case of tet(B), which codes for an efflux pump a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20002297.2019.1643204 |
| _version_ | 1819265820767813632 |
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| author | Alexandre Arredondo Gerard Àlvarez José Nart Carolina Mor Vanessa Blanc Rubén León |
| author_facet | Alexandre Arredondo Gerard Àlvarez José Nart Carolina Mor Vanessa Blanc Rubén León |
| author_sort | Alexandre Arredondo |
| collection | DOAJ |
| description | Tetracycline resistance can be achieved through tet genes, which code for efflux pumps, ribosomal protection proteins and inactivation enzymes. Some of these genes have only been described in either Gram-positive or Gram-negative bacteria. This is the case of tet(B), which codes for an efflux pump and, so far, had only been found in Gram-negative bacteria. In this study, tet(B) was detected in two clinical Streptococcus oralis strains isolated from the gingival sulci of two subjects. In both cases, the gene was completely sequenced, yielding 100% shared identity and coverage with other previously published sequences of tet(B). Moreover, we studied the expression of tet(B) using RT-qPCR in the isolates grown with and without tetracycline, detecting constitutive expression in only one of the isolates, with no signs of expression in the other one. This is the first time that the presence and expression of the tet(B) gene has been confirmed in Gram-positive bacteria, which highlights the potential of the genus Streptococcus to become a reservoir and a disseminator of antibiotic resistance genes in an environment so prone to horizontal gene transfer as is the oral biofilm. |
| first_indexed | 2024-12-23T20:51:27Z |
| format | Article |
| id | doaj.art-ebe0127189d14d18ac177f965a7a8545 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-23T20:51:27Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-ebe0127189d14d18ac177f965a7a85452022-12-21T17:31:39ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972019-01-0111110.1080/20002297.2019.16432041643204Detection and expression analysis of tet(B) in Streptococcus oralisAlexandre Arredondo0Gerard Àlvarez1José Nart2Carolina Mor3Vanessa Blanc4Rubén León5Dentaid Research CenterDentaid Research CenterUniversitat Internacional de CatalunyaUniversitat Internacional de CatalunyaDentaid Research CenterDentaid Research CenterTetracycline resistance can be achieved through tet genes, which code for efflux pumps, ribosomal protection proteins and inactivation enzymes. Some of these genes have only been described in either Gram-positive or Gram-negative bacteria. This is the case of tet(B), which codes for an efflux pump and, so far, had only been found in Gram-negative bacteria. In this study, tet(B) was detected in two clinical Streptococcus oralis strains isolated from the gingival sulci of two subjects. In both cases, the gene was completely sequenced, yielding 100% shared identity and coverage with other previously published sequences of tet(B). Moreover, we studied the expression of tet(B) using RT-qPCR in the isolates grown with and without tetracycline, detecting constitutive expression in only one of the isolates, with no signs of expression in the other one. This is the first time that the presence and expression of the tet(B) gene has been confirmed in Gram-positive bacteria, which highlights the potential of the genus Streptococcus to become a reservoir and a disseminator of antibiotic resistance genes in an environment so prone to horizontal gene transfer as is the oral biofilm.http://dx.doi.org/10.1080/20002297.2019.1643204tetracyclineantibiotic resistancestreptococcus oralistet(b)horizontal gene transfergene expression |
| spellingShingle | Alexandre Arredondo Gerard Àlvarez José Nart Carolina Mor Vanessa Blanc Rubén León Detection and expression analysis of tet(B) in Streptococcus oralis Journal of Oral Microbiology tetracycline antibiotic resistance streptococcus oralis tet(b) horizontal gene transfer gene expression |
| title | Detection and expression analysis of tet(B) in Streptococcus oralis |
| title_full | Detection and expression analysis of tet(B) in Streptococcus oralis |
| title_fullStr | Detection and expression analysis of tet(B) in Streptococcus oralis |
| title_full_unstemmed | Detection and expression analysis of tet(B) in Streptococcus oralis |
| title_short | Detection and expression analysis of tet(B) in Streptococcus oralis |
| title_sort | detection and expression analysis of tet b in streptococcus oralis |
| topic | tetracycline antibiotic resistance streptococcus oralis tet(b) horizontal gene transfer gene expression |
| url | http://dx.doi.org/10.1080/20002297.2019.1643204 |
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