Evaluation of microbial contamination on cuff syringe, cuff pressure gauge, and their surroundings in the operating room
Abstract Background Some institutions reuse cuff syringes and do not periodically sterilize cuff pressure gauges. Pathogenic bacterial contamination of such equipment may increase the probability of pathogen transmission to patients during anesthetic procedures. Therefore, microbial contamination on...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-12-01
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| Series: | JA Clinical Reports |
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| Online Access: | https://doi.org/10.1186/s40981-021-00486-0 |
| _version_ | 1818701603288383488 |
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| author | Rieko Oishi Kiwamu Nakamura Yoko Yahagi Kazutaka Ohashi Yukiko Takano Rie Harada Shinju Obara Satoki Inoue Keiji Kanemitsu Masahiro Murakawa |
| author_facet | Rieko Oishi Kiwamu Nakamura Yoko Yahagi Kazutaka Ohashi Yukiko Takano Rie Harada Shinju Obara Satoki Inoue Keiji Kanemitsu Masahiro Murakawa |
| author_sort | Rieko Oishi |
| collection | DOAJ |
| description | Abstract Background Some institutions reuse cuff syringes and do not periodically sterilize cuff pressure gauges. Pathogenic bacterial contamination of such equipment may increase the probability of pathogen transmission to patients during anesthetic procedures. Therefore, microbial contamination on cuff syringes, cuff pressure gauges, and their surroundings was assessed in the operating rooms of a university-affiliated tertiary care hospital in Japan. Methods This study was conducted between April and May 2019 in 14 operating suites at a hospital. The following sites in each operating suite were sampled: cuff syringe (inner/outer components), outer components of cuff pressure gauge, cuff syringe and cuff pressure gauge storage drawers, and computer mice. The swabs were directly streaked onto agar plates and incubated. Then, the bacterial species were identified using mass spectrometry. Results The highest bacterial isolation was observed in computer mice, followed by the outside of cuff pressure gauges and the drawers of cuff pressure gauges (92.9, 78.6, and 64.3%, respectively). Most of the identified bacteria belonged to the Bacillus species, with colonization rates of 85.7, 57.1, and 57.1% on computer mice, cuff pressure gauges, and cuff pressure gauge storage drawers, respectively. Coagulase-negative Staphylococcus was found in 35.7% of the specimens and was more prevalent on computer mice (71.4%), followed by on cuff pressure gauges (64.3%). Conclusion Anesthesiologists should be aware of the possible pathogen contamination risk from cuff syringes, cuff pressure gauges, or associated equipment and take appropriate infection control measures to minimize the risk of pathogenic transmission. |
| first_indexed | 2024-12-17T15:23:28Z |
| format | Article |
| id | doaj.art-ebe1206447f0424a83eeb3a38d4bff7e |
| institution | Directory Open Access Journal |
| issn | 2363-9024 |
| language | English |
| last_indexed | 2024-12-17T15:23:28Z |
| publishDate | 2021-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | JA Clinical Reports |
| spelling | doaj.art-ebe1206447f0424a83eeb3a38d4bff7e2022-12-21T21:43:21ZengSpringerOpenJA Clinical Reports2363-90242021-12-01711610.1186/s40981-021-00486-0Evaluation of microbial contamination on cuff syringe, cuff pressure gauge, and their surroundings in the operating roomRieko Oishi0Kiwamu Nakamura1Yoko Yahagi2Kazutaka Ohashi3Yukiko Takano4Rie Harada5Shinju Obara6Satoki Inoue7Keiji Kanemitsu8Masahiro Murakawa9Department of Anesthesiology, Fukushima Medical UniversityDepartment of Infection Control, Fukushima Medical UniversityFukushima Medical UniversityDepartment of Clinical Laboratory Medicine, Fukushima Medical University HospitalDepartment of Clinical Laboratory Medicine, Fukushima Medical University HospitalDepartment of Infection Control, Fukushima Medical UniversityDepartment of Anesthesiology, Fukushima Medical UniversityDepartment of Anesthesiology, Fukushima Medical UniversityDepartment of Infection Control, Fukushima Medical UniversityDepartment of Anesthesiology, Fukushima Medical UniversityAbstract Background Some institutions reuse cuff syringes and do not periodically sterilize cuff pressure gauges. Pathogenic bacterial contamination of such equipment may increase the probability of pathogen transmission to patients during anesthetic procedures. Therefore, microbial contamination on cuff syringes, cuff pressure gauges, and their surroundings was assessed in the operating rooms of a university-affiliated tertiary care hospital in Japan. Methods This study was conducted between April and May 2019 in 14 operating suites at a hospital. The following sites in each operating suite were sampled: cuff syringe (inner/outer components), outer components of cuff pressure gauge, cuff syringe and cuff pressure gauge storage drawers, and computer mice. The swabs were directly streaked onto agar plates and incubated. Then, the bacterial species were identified using mass spectrometry. Results The highest bacterial isolation was observed in computer mice, followed by the outside of cuff pressure gauges and the drawers of cuff pressure gauges (92.9, 78.6, and 64.3%, respectively). Most of the identified bacteria belonged to the Bacillus species, with colonization rates of 85.7, 57.1, and 57.1% on computer mice, cuff pressure gauges, and cuff pressure gauge storage drawers, respectively. Coagulase-negative Staphylococcus was found in 35.7% of the specimens and was more prevalent on computer mice (71.4%), followed by on cuff pressure gauges (64.3%). Conclusion Anesthesiologists should be aware of the possible pathogen contamination risk from cuff syringes, cuff pressure gauges, or associated equipment and take appropriate infection control measures to minimize the risk of pathogenic transmission.https://doi.org/10.1186/s40981-021-00486-0Hospital-acquired infections, HAIOperating rooms, ORsEquipment contaminationInfectionsOperating roomsBacterial infections |
| spellingShingle | Rieko Oishi Kiwamu Nakamura Yoko Yahagi Kazutaka Ohashi Yukiko Takano Rie Harada Shinju Obara Satoki Inoue Keiji Kanemitsu Masahiro Murakawa Evaluation of microbial contamination on cuff syringe, cuff pressure gauge, and their surroundings in the operating room JA Clinical Reports Hospital-acquired infections, HAI Operating rooms, ORs Equipment contamination Infections Operating rooms Bacterial infections |
| title | Evaluation of microbial contamination on cuff syringe, cuff pressure gauge, and their surroundings in the operating room |
| title_full | Evaluation of microbial contamination on cuff syringe, cuff pressure gauge, and their surroundings in the operating room |
| title_fullStr | Evaluation of microbial contamination on cuff syringe, cuff pressure gauge, and their surroundings in the operating room |
| title_full_unstemmed | Evaluation of microbial contamination on cuff syringe, cuff pressure gauge, and their surroundings in the operating room |
| title_short | Evaluation of microbial contamination on cuff syringe, cuff pressure gauge, and their surroundings in the operating room |
| title_sort | evaluation of microbial contamination on cuff syringe cuff pressure gauge and their surroundings in the operating room |
| topic | Hospital-acquired infections, HAI Operating rooms, ORs Equipment contamination Infections Operating rooms Bacterial infections |
| url | https://doi.org/10.1186/s40981-021-00486-0 |
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