Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae

Infection of root lession nematode, Pratylenchus coffeae, becomes an important factor causing yield loss in coffee production in Indonesia. Biological control of P. coffeae needs to be developed to meet the requirement of environmentally save crop production.  The research was conducted to evaluate...

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Main Authors: Dwi Halimah, Abdul Munif, Giyanto Giyanto
Format: Article
Language:Indonesian
Published: Perhimpunan Fitopatologi Indonesia 2016-05-01
Series:Jurnal Fitopatologi Indonesia
Subjects:
Online Access:https://journal.ipb.ac.id/index.php/jfiti/article/view/12044
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author Dwi Halimah
Abdul Munif
Giyanto Giyanto
author_facet Dwi Halimah
Abdul Munif
Giyanto Giyanto
author_sort Dwi Halimah
collection DOAJ
description Infection of root lession nematode, Pratylenchus coffeae, becomes an important factor causing yield loss in coffee production in Indonesia. Biological control of P. coffeae needs to be developed to meet the requirement of environmentally save crop production.  The research was conducted to evaluate 3 selected endophytic bacteria isolates, Ochrobactrum intermedium DSQ5, Klebsiella oxytoca NGB-FR 50, and Bacillus  subtilis AIMST 10.T18.1, from coffee in controlling P. coffeae. Research methods involved pathogenicity test based on hypersensitive reaction and haemolysis test, and evaluation of their ability to suppress P. coffeae in vitro and in planta. The hypersensitive and haemolysis reactions indicated that these 3 isolates showed negative result both to plant and human. In vitro assays showed that two isolates, K. oxytoca - C939A32 and O. intermedium - C939A3, could suppress P. coffeae populations by 66.7% and 100%. Those results correlated positively with in planta assay’s result.
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spelling doaj.art-ebe1b8250c5a4e73a3450fd688f078762023-01-04T02:44:14ZindPerhimpunan Fitopatologi IndonesiaJurnal Fitopatologi Indonesia0215-79502339-24792016-05-0112210.14692/jfi.12.2.629617Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeaeDwi Halimah0Abdul Munif1Giyanto Giyanto2Institut Pertanian BogorInstitut Pertanian BogorInstitut Pertanian BogorInfection of root lession nematode, Pratylenchus coffeae, becomes an important factor causing yield loss in coffee production in Indonesia. Biological control of P. coffeae needs to be developed to meet the requirement of environmentally save crop production.  The research was conducted to evaluate 3 selected endophytic bacteria isolates, Ochrobactrum intermedium DSQ5, Klebsiella oxytoca NGB-FR 50, and Bacillus  subtilis AIMST 10.T18.1, from coffee in controlling P. coffeae. Research methods involved pathogenicity test based on hypersensitive reaction and haemolysis test, and evaluation of their ability to suppress P. coffeae in vitro and in planta. The hypersensitive and haemolysis reactions indicated that these 3 isolates showed negative result both to plant and human. In vitro assays showed that two isolates, K. oxytoca - C939A32 and O. intermedium - C939A3, could suppress P. coffeae populations by 66.7% and 100%. Those results correlated positively with in planta assay’s result.https://journal.ipb.ac.id/index.php/jfiti/article/view/12044endophityc bacteriahaemolysis testhypersensitive reactionpathogenicity test
spellingShingle Dwi Halimah
Abdul Munif
Giyanto Giyanto
Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae
Jurnal Fitopatologi Indonesia
endophityc bacteria
haemolysis test
hypersensitive reaction
pathogenicity test
title Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae
title_full Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae
title_fullStr Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae
title_full_unstemmed Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae
title_short Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae
title_sort potensi ochrobactrum intermedium c939a31 klebsiella oxytoca c939a32 bacillus subtilis i308a32 asal tanaman kopi untuk mengendalikan nematoda luka akar pratylenchus coffeae
topic endophityc bacteria
haemolysis test
hypersensitive reaction
pathogenicity test
url https://journal.ipb.ac.id/index.php/jfiti/article/view/12044
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AT abdulmunif potensiochrobactrumintermediumc939a31klebsiellaoxytocac939a32bacillussubtilisi308a32asaltanamankopiuntukmengendalikannematodalukaakarpratylenchuscoffeae
AT giyantogiyanto potensiochrobactrumintermediumc939a31klebsiellaoxytocac939a32bacillussubtilisi308a32asaltanamankopiuntukmengendalikannematodalukaakarpratylenchuscoffeae