Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae
Infection of root lession nematode, Pratylenchus coffeae, becomes an important factor causing yield loss in coffee production in Indonesia. Biological control of P. coffeae needs to be developed to meet the requirement of environmentally save crop production. The research was conducted to evaluate...
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Format: | Article |
Language: | Indonesian |
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Perhimpunan Fitopatologi Indonesia
2016-05-01
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Series: | Jurnal Fitopatologi Indonesia |
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Online Access: | https://journal.ipb.ac.id/index.php/jfiti/article/view/12044 |
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author | Dwi Halimah Abdul Munif Giyanto Giyanto |
author_facet | Dwi Halimah Abdul Munif Giyanto Giyanto |
author_sort | Dwi Halimah |
collection | DOAJ |
description | Infection of root lession nematode, Pratylenchus coffeae, becomes an important factor causing yield loss in coffee production in Indonesia. Biological control of P. coffeae needs to be developed to meet the requirement of environmentally save crop production. The research was conducted to evaluate 3 selected endophytic bacteria isolates, Ochrobactrum intermedium DSQ5, Klebsiella oxytoca NGB-FR 50, and Bacillus subtilis AIMST 10.T18.1, from coffee in controlling P. coffeae. Research methods involved pathogenicity test based on hypersensitive reaction and haemolysis test, and evaluation of their ability to suppress P. coffeae in vitro and in planta. The hypersensitive and haemolysis reactions indicated that these 3 isolates showed negative result both to plant and human. In vitro assays showed that two isolates, K. oxytoca - C939A32 and O. intermedium - C939A3, could suppress P. coffeae populations by 66.7% and 100%. Those results correlated positively with in planta assay’s result. |
first_indexed | 2024-04-11T01:16:49Z |
format | Article |
id | doaj.art-ebe1b8250c5a4e73a3450fd688f07876 |
institution | Directory Open Access Journal |
issn | 0215-7950 2339-2479 |
language | Indonesian |
last_indexed | 2024-04-11T01:16:49Z |
publishDate | 2016-05-01 |
publisher | Perhimpunan Fitopatologi Indonesia |
record_format | Article |
series | Jurnal Fitopatologi Indonesia |
spelling | doaj.art-ebe1b8250c5a4e73a3450fd688f078762023-01-04T02:44:14ZindPerhimpunan Fitopatologi IndonesiaJurnal Fitopatologi Indonesia0215-79502339-24792016-05-0112210.14692/jfi.12.2.629617Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeaeDwi Halimah0Abdul Munif1Giyanto Giyanto2Institut Pertanian BogorInstitut Pertanian BogorInstitut Pertanian BogorInfection of root lession nematode, Pratylenchus coffeae, becomes an important factor causing yield loss in coffee production in Indonesia. Biological control of P. coffeae needs to be developed to meet the requirement of environmentally save crop production. The research was conducted to evaluate 3 selected endophytic bacteria isolates, Ochrobactrum intermedium DSQ5, Klebsiella oxytoca NGB-FR 50, and Bacillus subtilis AIMST 10.T18.1, from coffee in controlling P. coffeae. Research methods involved pathogenicity test based on hypersensitive reaction and haemolysis test, and evaluation of their ability to suppress P. coffeae in vitro and in planta. The hypersensitive and haemolysis reactions indicated that these 3 isolates showed negative result both to plant and human. In vitro assays showed that two isolates, K. oxytoca - C939A32 and O. intermedium - C939A3, could suppress P. coffeae populations by 66.7% and 100%. Those results correlated positively with in planta assay’s result.https://journal.ipb.ac.id/index.php/jfiti/article/view/12044endophityc bacteriahaemolysis testhypersensitive reactionpathogenicity test |
spellingShingle | Dwi Halimah Abdul Munif Giyanto Giyanto Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae Jurnal Fitopatologi Indonesia endophityc bacteria haemolysis test hypersensitive reaction pathogenicity test |
title | Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae |
title_full | Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae |
title_fullStr | Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae |
title_full_unstemmed | Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae |
title_short | Potensi Ochrobactrum intermedium - C939A31, Klebsiella oxytoca - C939A32, Bacillus subtilis- I308A32 Asal Tanaman Kopi untuk Mengendalikan Nematoda Luka Akar Pratylenchus coffeae |
title_sort | potensi ochrobactrum intermedium c939a31 klebsiella oxytoca c939a32 bacillus subtilis i308a32 asal tanaman kopi untuk mengendalikan nematoda luka akar pratylenchus coffeae |
topic | endophityc bacteria haemolysis test hypersensitive reaction pathogenicity test |
url | https://journal.ipb.ac.id/index.php/jfiti/article/view/12044 |
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