Overexpression of Nitrate Transporter 1/Peptide Gene <i>OsNPF7.6</i> Increases Rice Yield and Nitrogen Use Efficiency
Overuse of nitrogen fertilizer in fields has raised production costs, and caused environmental problems. Improving nitrogen use efficiency (NUE) of rice is essential for sustainable agriculture. Here we report the cloning, characterization and roles for rice of <i>OsNPF7.6</i>, a member...
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MDPI AG
2022-11-01
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| author | Min Zhang Liuru Lai Xintong Liu Jiajia Liu Ruifang Liu Yamei Wang Jindong Liu Jingguang Chen |
| author_facet | Min Zhang Liuru Lai Xintong Liu Jiajia Liu Ruifang Liu Yamei Wang Jindong Liu Jingguang Chen |
| author_sort | Min Zhang |
| collection | DOAJ |
| description | Overuse of nitrogen fertilizer in fields has raised production costs, and caused environmental problems. Improving nitrogen use efficiency (NUE) of rice is essential for sustainable agriculture. Here we report the cloning, characterization and roles for rice of <i>OsNPF7.6</i>, a member of the nitrate transporter 1/peptide transporter family (NPF). The OsNPF7.6 protein is located in the plasma membrane, expressed in each tissue at all stages and is significantly regulated by nitrate in rice. Our study shows that the overexpression of <i>OsNPF7.6</i> can increase the nitrate uptake rate of rice. Additionally, field experiments showed that <i>OsNPF7.6</i> overexpression increased the total tiller number per plant and the grain weight per panicle, thereby improving grain yield and agronomic NUE in rice. Thus, <i>OsNPF7.6</i> can be applied to be a novel target gene for breeding rice varieties with high NUE, and provide a reference for breeding higher yielding rice. |
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| language | English |
| last_indexed | 2024-03-09T16:11:30Z |
| publishDate | 2022-11-01 |
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| spelling | doaj.art-ebe3cbf410e04cbbb9834f74a8353dcd2023-11-24T16:11:30ZengMDPI AGLife2075-17292022-11-011212198110.3390/life12121981Overexpression of Nitrate Transporter 1/Peptide Gene <i>OsNPF7.6</i> Increases Rice Yield and Nitrogen Use EfficiencyMin Zhang0Liuru Lai1Xintong Liu2Jiajia Liu3Ruifang Liu4Yamei Wang5Jindong Liu6Jingguang Chen7College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaSchool of Agriculture, Shenzhen Campus, Sun Yat-sen University, Shenzhen 518107, ChinaSchool of Agriculture, Shenzhen Campus, Sun Yat-sen University, Shenzhen 518107, ChinaShandong Jinchunyu Seed Technology Co., Ltd., Jining 272200, ChinaThe High School Affiliated to Renmin University of China, Shenzhen 518119, ChinaSchool of Agriculture, Shenzhen Campus, Sun Yat-sen University, Shenzhen 518107, ChinaInstitute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing 100081, ChinaSchool of Agriculture, Shenzhen Campus, Sun Yat-sen University, Shenzhen 518107, ChinaOveruse of nitrogen fertilizer in fields has raised production costs, and caused environmental problems. Improving nitrogen use efficiency (NUE) of rice is essential for sustainable agriculture. Here we report the cloning, characterization and roles for rice of <i>OsNPF7.6</i>, a member of the nitrate transporter 1/peptide transporter family (NPF). The OsNPF7.6 protein is located in the plasma membrane, expressed in each tissue at all stages and is significantly regulated by nitrate in rice. Our study shows that the overexpression of <i>OsNPF7.6</i> can increase the nitrate uptake rate of rice. Additionally, field experiments showed that <i>OsNPF7.6</i> overexpression increased the total tiller number per plant and the grain weight per panicle, thereby improving grain yield and agronomic NUE in rice. Thus, <i>OsNPF7.6</i> can be applied to be a novel target gene for breeding rice varieties with high NUE, and provide a reference for breeding higher yielding rice.https://www.mdpi.com/2075-1729/12/12/1981<sup>15</sup>NO<sub>3</sub><sup>−</sup> influxagronomic nitrogen use efficiencyfunctional analysis<i>OsNPF7.6</i><i>Oryza sativa</i> |
| spellingShingle | Min Zhang Liuru Lai Xintong Liu Jiajia Liu Ruifang Liu Yamei Wang Jindong Liu Jingguang Chen Overexpression of Nitrate Transporter 1/Peptide Gene <i>OsNPF7.6</i> Increases Rice Yield and Nitrogen Use Efficiency Life <sup>15</sup>NO<sub>3</sub><sup>−</sup> influx agronomic nitrogen use efficiency functional analysis <i>OsNPF7.6</i> <i>Oryza sativa</i> |
| title | Overexpression of Nitrate Transporter 1/Peptide Gene <i>OsNPF7.6</i> Increases Rice Yield and Nitrogen Use Efficiency |
| title_full | Overexpression of Nitrate Transporter 1/Peptide Gene <i>OsNPF7.6</i> Increases Rice Yield and Nitrogen Use Efficiency |
| title_fullStr | Overexpression of Nitrate Transporter 1/Peptide Gene <i>OsNPF7.6</i> Increases Rice Yield and Nitrogen Use Efficiency |
| title_full_unstemmed | Overexpression of Nitrate Transporter 1/Peptide Gene <i>OsNPF7.6</i> Increases Rice Yield and Nitrogen Use Efficiency |
| title_short | Overexpression of Nitrate Transporter 1/Peptide Gene <i>OsNPF7.6</i> Increases Rice Yield and Nitrogen Use Efficiency |
| title_sort | overexpression of nitrate transporter 1 peptide gene i osnpf7 6 i increases rice yield and nitrogen use efficiency |
| topic | <sup>15</sup>NO<sub>3</sub><sup>−</sup> influx agronomic nitrogen use efficiency functional analysis <i>OsNPF7.6</i> <i>Oryza sativa</i> |
| url | https://www.mdpi.com/2075-1729/12/12/1981 |
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