Interaction between NBS1 and the mTOR/Rictor/SIN1 complex through specific domains.
Nijmegen breakage syndrome (NBS) is a chromosomal-instability syndrome. The NBS gene product, NBS1 (p95 or nibrin), is a part of the Mre11-Rad50-NBS1 complex. SIN1 is a component of the mTOR/Rictor/SIN1 complex mediating the activation of Akt. Here we show that NBS1 interacted with mTOR, Rictor, and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3675082?pdf=render |
| _version_ | 1819082391167172608 |
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| author | Jian-Qiu Wang Jian-Hong Chen Yen-Chung Chen Mei-Yu Chen Chia-Ying Hsieh Shu-Chun Teng Kou-Juey Wu |
| author_facet | Jian-Qiu Wang Jian-Hong Chen Yen-Chung Chen Mei-Yu Chen Chia-Ying Hsieh Shu-Chun Teng Kou-Juey Wu |
| author_sort | Jian-Qiu Wang |
| collection | DOAJ |
| description | Nijmegen breakage syndrome (NBS) is a chromosomal-instability syndrome. The NBS gene product, NBS1 (p95 or nibrin), is a part of the Mre11-Rad50-NBS1 complex. SIN1 is a component of the mTOR/Rictor/SIN1 complex mediating the activation of Akt. Here we show that NBS1 interacted with mTOR, Rictor, and SIN1. The specific domains of mTOR, Rictor, or SIN1 interacted with the internal domain (a.a. 221-402) of NBS1. Sucrose density gradient showed that NBS1 was located in the same fractions as the mTOR/Rictor/SIN1 complex. Knockdown of NBS1 decreased the levels of phosphorylated Akt and its downstream targets. Ionizing radiation (IR) increased the NBS1 levels and activated Akt activity. These results demonstrate that NBS1 interacts with the mTOR/Rictor/SIN1 complex through the a.a. 221-402 domain and contributes to the activation of Akt activity. |
| first_indexed | 2024-12-21T20:15:55Z |
| format | Article |
| id | doaj.art-ebea836a5483424687678327e2df0e1e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T20:15:55Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ebea836a5483424687678327e2df0e1e2022-12-21T18:51:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6558610.1371/journal.pone.0065586Interaction between NBS1 and the mTOR/Rictor/SIN1 complex through specific domains.Jian-Qiu WangJian-Hong ChenYen-Chung ChenMei-Yu ChenChia-Ying HsiehShu-Chun TengKou-Juey WuNijmegen breakage syndrome (NBS) is a chromosomal-instability syndrome. The NBS gene product, NBS1 (p95 or nibrin), is a part of the Mre11-Rad50-NBS1 complex. SIN1 is a component of the mTOR/Rictor/SIN1 complex mediating the activation of Akt. Here we show that NBS1 interacted with mTOR, Rictor, and SIN1. The specific domains of mTOR, Rictor, or SIN1 interacted with the internal domain (a.a. 221-402) of NBS1. Sucrose density gradient showed that NBS1 was located in the same fractions as the mTOR/Rictor/SIN1 complex. Knockdown of NBS1 decreased the levels of phosphorylated Akt and its downstream targets. Ionizing radiation (IR) increased the NBS1 levels and activated Akt activity. These results demonstrate that NBS1 interacts with the mTOR/Rictor/SIN1 complex through the a.a. 221-402 domain and contributes to the activation of Akt activity.http://europepmc.org/articles/PMC3675082?pdf=render |
| spellingShingle | Jian-Qiu Wang Jian-Hong Chen Yen-Chung Chen Mei-Yu Chen Chia-Ying Hsieh Shu-Chun Teng Kou-Juey Wu Interaction between NBS1 and the mTOR/Rictor/SIN1 complex through specific domains. PLoS ONE |
| title | Interaction between NBS1 and the mTOR/Rictor/SIN1 complex through specific domains. |
| title_full | Interaction between NBS1 and the mTOR/Rictor/SIN1 complex through specific domains. |
| title_fullStr | Interaction between NBS1 and the mTOR/Rictor/SIN1 complex through specific domains. |
| title_full_unstemmed | Interaction between NBS1 and the mTOR/Rictor/SIN1 complex through specific domains. |
| title_short | Interaction between NBS1 and the mTOR/Rictor/SIN1 complex through specific domains. |
| title_sort | interaction between nbs1 and the mtor rictor sin1 complex through specific domains |
| url | http://europepmc.org/articles/PMC3675082?pdf=render |
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