Potential of diallyl sulfide from bulbs of Allium parvum to inhibit the growth and biofilm formation in Malassezia furfur MTCC 1374
The bulbs of Allium parvum are used as a flavoring agent in diet and are conventionally assumed to treat skin infections. The current study evaluates the phytochemicals from organic extract of A. parvum and their antifungal activity against biofilm-forming dermatophytic fungi Malassezia furfur MTCC...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2024-03-01
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| Series: | Notulae Scientia Biologicae |
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| Online Access: | https://www.notulaebiologicae.ro/index.php/nsb/article/view/11758 |
| _version_ | 1797231371185291264 |
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| author | Saravana P. KUMARI E. MEIYAPPAN |
| author_facet | Saravana P. KUMARI E. MEIYAPPAN |
| author_sort | Saravana P. KUMARI |
| collection | DOAJ |
| description |
The bulbs of Allium parvum are used as a flavoring agent in diet and are conventionally assumed to treat skin infections. The current study evaluates the phytochemicals from organic extract of A. parvum and their antifungal activity against biofilm-forming dermatophytic fungi Malassezia furfur MTCC 1374, yeast found in the normal flora of the skin, causes superficial infections, systemic infections like pulmonary infections, fungemia, etc., and implant-associated infection by biofilm formation. Biofilm formation enables the fungi to escape from antifungal agents and renders resistance to antifungal agents. The phytochemical assay for the organic extract of the onion bulb documented that A. parvum contains phenol (58 + 0.1 mg GAE/g), flavonoids (112 + 0.12 mg QE/g), saponins (1.45 + 0.1 mg AE/g), and tannins (4.1 + 0.03 g TA/g). The FT-IR and GC-MS analysis revealed the presence of specific compounds, alkaloids such as glycosides and quinolones, plant flavonoids such as 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-ester, phenolics, saponins such as butanoic acid and gluconic acid, and organic sulfur compounds such as diallyl sulfide, thiopyran tetrahydro-ester, allyl methyl trisulfide, and allyl methyl di sulfide. These compounds inhibited the growth of M. furfur at a minimum inhibitory concentration (MIC) value of 6.25 mg/ml and its biofilm at a minimum biofilm inhibitory concentration (MBIC) value of 12.5 mg/ml. Further studies required to explore the possible mechanism in controlling the biofilm, so, as to recommend as fungal control agent in medical sector.
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| first_indexed | 2024-04-24T15:43:19Z |
| format | Article |
| id | doaj.art-ebec1a19278349e395da073884932dfe |
| institution | Directory Open Access Journal |
| issn | 2067-3264 |
| language | English |
| last_indexed | 2024-04-24T15:43:19Z |
| publishDate | 2024-03-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-ebec1a19278349e395da073884932dfe2024-04-01T18:32:20ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32642024-03-0116110.55779/nsb16111758Potential of diallyl sulfide from bulbs of Allium parvum to inhibit the growth and biofilm formation in Malassezia furfur MTCC 1374Saravana P. KUMARI0E. MEIYAPPAN1RVS College of Arts and Science, Department of Microbiology, CoimbatoreRVS College of Arts and Science, Department of Microbiology, Coimbatore The bulbs of Allium parvum are used as a flavoring agent in diet and are conventionally assumed to treat skin infections. The current study evaluates the phytochemicals from organic extract of A. parvum and their antifungal activity against biofilm-forming dermatophytic fungi Malassezia furfur MTCC 1374, yeast found in the normal flora of the skin, causes superficial infections, systemic infections like pulmonary infections, fungemia, etc., and implant-associated infection by biofilm formation. Biofilm formation enables the fungi to escape from antifungal agents and renders resistance to antifungal agents. The phytochemical assay for the organic extract of the onion bulb documented that A. parvum contains phenol (58 + 0.1 mg GAE/g), flavonoids (112 + 0.12 mg QE/g), saponins (1.45 + 0.1 mg AE/g), and tannins (4.1 + 0.03 g TA/g). The FT-IR and GC-MS analysis revealed the presence of specific compounds, alkaloids such as glycosides and quinolones, plant flavonoids such as 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-ester, phenolics, saponins such as butanoic acid and gluconic acid, and organic sulfur compounds such as diallyl sulfide, thiopyran tetrahydro-ester, allyl methyl trisulfide, and allyl methyl di sulfide. These compounds inhibited the growth of M. furfur at a minimum inhibitory concentration (MIC) value of 6.25 mg/ml and its biofilm at a minimum biofilm inhibitory concentration (MBIC) value of 12.5 mg/ml. Further studies required to explore the possible mechanism in controlling the biofilm, so, as to recommend as fungal control agent in medical sector. https://www.notulaebiologicae.ro/index.php/nsb/article/view/11758Allium parvumbioautographyMalassezia furfurminimum biofilm inhibitory assayminimum inhibitory concentration |
| spellingShingle | Saravana P. KUMARI E. MEIYAPPAN Potential of diallyl sulfide from bulbs of Allium parvum to inhibit the growth and biofilm formation in Malassezia furfur MTCC 1374 Notulae Scientia Biologicae Allium parvum bioautography Malassezia furfur minimum biofilm inhibitory assay minimum inhibitory concentration |
| title | Potential of diallyl sulfide from bulbs of Allium parvum to inhibit the growth and biofilm formation in Malassezia furfur MTCC 1374 |
| title_full | Potential of diallyl sulfide from bulbs of Allium parvum to inhibit the growth and biofilm formation in Malassezia furfur MTCC 1374 |
| title_fullStr | Potential of diallyl sulfide from bulbs of Allium parvum to inhibit the growth and biofilm formation in Malassezia furfur MTCC 1374 |
| title_full_unstemmed | Potential of diallyl sulfide from bulbs of Allium parvum to inhibit the growth and biofilm formation in Malassezia furfur MTCC 1374 |
| title_short | Potential of diallyl sulfide from bulbs of Allium parvum to inhibit the growth and biofilm formation in Malassezia furfur MTCC 1374 |
| title_sort | potential of diallyl sulfide from bulbs of allium parvum to inhibit the growth and biofilm formation in malassezia furfur mtcc 1374 |
| topic | Allium parvum bioautography Malassezia furfur minimum biofilm inhibitory assay minimum inhibitory concentration |
| url | https://www.notulaebiologicae.ro/index.php/nsb/article/view/11758 |
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