Time-Course Transcriptome Profiling of a Poxvirus Using Long-Read Full-Length Assay

Time-Course Transcriptome Profiling of a Poxvirus Using Long-Read Full-Length Assay

Viral transcriptomes that are determined using first- and second-generation sequencing techniques are incomplete. Due to the short read length, these methods are inefficient or fail to distinguish between transcript isoforms, polycistronic RNAs, and transcriptional overlaps and readthroughs. Additio...

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Bibliographic Details
Main Authors: Dóra Tombácz, István Prazsák, Gábor Torma, Zsolt Csabai, Zsolt Balázs, Norbert Moldován, Béla Dénes, Michael Snyder, Zsolt Boldogkői
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Pathogens
Subjects:
vaccinia virus
long-read sequencing
nanopore sequencing
transcriptome profiling
gene expression
Online Access:https://www.mdpi.com/2076-0817/10/8/919

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