Characterization and Pharmacological Activities of Jellyfish, Chrysaora hysoscella Captured in Bushehr Port, Iran
Background: Cutaneous reactions like pruritus and erythema are common in warm months of the year in Bushehr Port, Persian Gulf, Iran due to jellyfish envenomation. This study reports isolation of the Chrysaora hysoscella nematocysts and evaluating its pharmacological activities during a bloom in 201...
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| Format: | Article |
| Language: | English |
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Arak University of Medical Sciences
2016-08-01
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| Series: | Iranian Journal of Toxicology |
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| Online Access: | http://ijt.arakmu.ac.ir/browse.php?a_id=508&slc_lang=en&sid=1&ftxt=1 |
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| author | Somayyeh Gharibi Iraj Nabipour Euikyung Kim Seyede Maryam Ghafari Seyed Mehdi Hoseiny Mostafa Kamyab Ramin Seyedian |
| author_facet | Somayyeh Gharibi Iraj Nabipour Euikyung Kim Seyede Maryam Ghafari Seyed Mehdi Hoseiny Mostafa Kamyab Ramin Seyedian |
| author_sort | Somayyeh Gharibi |
| collection | DOAJ |
| description | Background: Cutaneous reactions like pruritus and erythema are common in warm months of the year in Bushehr Port, Persian Gulf, Iran due to jellyfish envenomation. This study reports isolation of the Chrysaora hysoscella nematocysts and evaluating its pharmacological activities during a bloom in 2013.
Methods: The venom of C. hysoscella captured in Jofre area in Bushehr port was analyzed. The electrophoretic profile was assessed by SDS-PAGE (12.5%) and the crude sample was analyzed using reverse phase HPLC. Caseinase activity was also determined.
Results: After separation of tentacles and isolation of their nematocysts, three different major protein components were revealed at 72-250 kDa with SDS-PAGE, signifying the presence of peptides in its venom. Two major peaks at 8.62 and 11.23 min were observed in reverse phase HPLC of the crude venom denoting protease peptide structural identities. Caseinase activity of C. hysoscella's venom was extremely low as compared with other jellyfish venoms.
Conclusion: This was the first report on the structural examination of jellyfish in Persian Gulf and may pave the way for determination and separation of destructive enzymes inducing cutaneous reactions in fishermen and swimmers. |
| first_indexed | 2024-12-12T22:59:49Z |
| format | Article |
| id | doaj.art-ebf04cc437464831b4f9f587257fa00c |
| institution | Directory Open Access Journal |
| issn | 2008-2967 2251-9459 |
| language | English |
| last_indexed | 2024-12-12T22:59:49Z |
| publishDate | 2016-08-01 |
| publisher | Arak University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Toxicology |
| spelling | doaj.art-ebf04cc437464831b4f9f587257fa00c2022-12-22T00:08:52ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592016-08-01105710Characterization and Pharmacological Activities of Jellyfish, Chrysaora hysoscella Captured in Bushehr Port, IranSomayyeh Gharibi0 Iraj Nabipour1Euikyung Kim 2Seyede Maryam Ghafari 3Seyed Mehdi Hoseiny 4Mostafa Kamyab 5Ramin Seyedian6Department of Marine Toxicology, The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran.Department of Marine Toxicology, The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran.PhD of Toxicology, College of Veterinary Medicine, Gyeoungsang National University, Jinju, South Korea.Department of Parasitology, Pasteur Institute, Tehran, Iran.Department of Marine Toxicology, The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran.Department of Biological Sciences, Shahid Beheshti University, Tehran, Iran.Department of Marine Toxicology, The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran.* Corresponding Author: E-mail: raminseyedian@gmail.comBackground: Cutaneous reactions like pruritus and erythema are common in warm months of the year in Bushehr Port, Persian Gulf, Iran due to jellyfish envenomation. This study reports isolation of the Chrysaora hysoscella nematocysts and evaluating its pharmacological activities during a bloom in 2013. Methods: The venom of C. hysoscella captured in Jofre area in Bushehr port was analyzed. The electrophoretic profile was assessed by SDS-PAGE (12.5%) and the crude sample was analyzed using reverse phase HPLC. Caseinase activity was also determined. Results: After separation of tentacles and isolation of their nematocysts, three different major protein components were revealed at 72-250 kDa with SDS-PAGE, signifying the presence of peptides in its venom. Two major peaks at 8.62 and 11.23 min were observed in reverse phase HPLC of the crude venom denoting protease peptide structural identities. Caseinase activity of C. hysoscella's venom was extremely low as compared with other jellyfish venoms. Conclusion: This was the first report on the structural examination of jellyfish in Persian Gulf and may pave the way for determination and separation of destructive enzymes inducing cutaneous reactions in fishermen and swimmers.http://ijt.arakmu.ac.ir/browse.php?a_id=508&slc_lang=en&sid=1&ftxt=1CaseinaseCnidarian VenomsNematocystScyphozoaSea Nettle |
| spellingShingle | Somayyeh Gharibi Iraj Nabipour Euikyung Kim Seyede Maryam Ghafari Seyed Mehdi Hoseiny Mostafa Kamyab Ramin Seyedian Characterization and Pharmacological Activities of Jellyfish, Chrysaora hysoscella Captured in Bushehr Port, Iran Iranian Journal of Toxicology Caseinase Cnidarian Venoms Nematocyst Scyphozoa Sea Nettle |
| title | Characterization and Pharmacological Activities of Jellyfish, Chrysaora hysoscella Captured in Bushehr Port, Iran |
| title_full | Characterization and Pharmacological Activities of Jellyfish, Chrysaora hysoscella Captured in Bushehr Port, Iran |
| title_fullStr | Characterization and Pharmacological Activities of Jellyfish, Chrysaora hysoscella Captured in Bushehr Port, Iran |
| title_full_unstemmed | Characterization and Pharmacological Activities of Jellyfish, Chrysaora hysoscella Captured in Bushehr Port, Iran |
| title_short | Characterization and Pharmacological Activities of Jellyfish, Chrysaora hysoscella Captured in Bushehr Port, Iran |
| title_sort | characterization and pharmacological activities of jellyfish chrysaora hysoscella captured in bushehr port iran |
| topic | Caseinase Cnidarian Venoms Nematocyst Scyphozoa Sea Nettle |
| url | http://ijt.arakmu.ac.ir/browse.php?a_id=508&slc_lang=en&sid=1&ftxt=1 |
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