S-polarized light-sheets improve resolution and light-efficiency in oblique plane microscopy
Abstract Oblique plane microscopy (OPM) offers 3D optically sectioned imaging with high spatial- and temporal-resolution while enabling conventional sample mounting. The technique uses a concatenation of three microscopes, two for remote focusing and a tilted tertiary microscope, often including an...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-53900-9 |
| _version_ | 1797274756708302848 |
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| author | Jon-Richard Sommernes Alfred Millett-Sikking Florian Ströhl |
| author_facet | Jon-Richard Sommernes Alfred Millett-Sikking Florian Ströhl |
| author_sort | Jon-Richard Sommernes |
| collection | DOAJ |
| description | Abstract Oblique plane microscopy (OPM) offers 3D optically sectioned imaging with high spatial- and temporal-resolution while enabling conventional sample mounting. The technique uses a concatenation of three microscopes, two for remote focusing and a tilted tertiary microscope, often including an immersion objective, to image an oblique sample plane. This design induces Fresnel reflections and a reduced effective aperture, thus impacting the resolution and light efficiency of the system. Using vectorial diffraction simulations, the system performance was characterized based on illumination angle and polarization, signal to noise ratio, and refractive index of the tertiary objective immersion. We show that for samples with high fluorescent anisotropy, s-polarized light-sheets yield higher average resolution for all system configurations, as well as higher light-efficiency. We also provide a tool for performance characterization of arbitrary light-sheet imaging systems. |
| first_indexed | 2024-03-07T15:02:51Z |
| format | Article |
| id | doaj.art-ebf137279e244b8eadc52bbe33da7123 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:02:51Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ebf137279e244b8eadc52bbe33da71232024-03-05T19:04:46ZengNature PortfolioScientific Reports2045-23222024-02-011411910.1038/s41598-024-53900-9S-polarized light-sheets improve resolution and light-efficiency in oblique plane microscopyJon-Richard Sommernes0Alfred Millett-Sikking1Florian Ströhl2Department of Physics and Technology, UiT The Arctic University of NorwayCalico Life Sciences LLCDepartment of Physics and Technology, UiT The Arctic University of NorwayAbstract Oblique plane microscopy (OPM) offers 3D optically sectioned imaging with high spatial- and temporal-resolution while enabling conventional sample mounting. The technique uses a concatenation of three microscopes, two for remote focusing and a tilted tertiary microscope, often including an immersion objective, to image an oblique sample plane. This design induces Fresnel reflections and a reduced effective aperture, thus impacting the resolution and light efficiency of the system. Using vectorial diffraction simulations, the system performance was characterized based on illumination angle and polarization, signal to noise ratio, and refractive index of the tertiary objective immersion. We show that for samples with high fluorescent anisotropy, s-polarized light-sheets yield higher average resolution for all system configurations, as well as higher light-efficiency. We also provide a tool for performance characterization of arbitrary light-sheet imaging systems.https://doi.org/10.1038/s41598-024-53900-9 |
| spellingShingle | Jon-Richard Sommernes Alfred Millett-Sikking Florian Ströhl S-polarized light-sheets improve resolution and light-efficiency in oblique plane microscopy Scientific Reports |
| title | S-polarized light-sheets improve resolution and light-efficiency in oblique plane microscopy |
| title_full | S-polarized light-sheets improve resolution and light-efficiency in oblique plane microscopy |
| title_fullStr | S-polarized light-sheets improve resolution and light-efficiency in oblique plane microscopy |
| title_full_unstemmed | S-polarized light-sheets improve resolution and light-efficiency in oblique plane microscopy |
| title_short | S-polarized light-sheets improve resolution and light-efficiency in oblique plane microscopy |
| title_sort | s polarized light sheets improve resolution and light efficiency in oblique plane microscopy |
| url | https://doi.org/10.1038/s41598-024-53900-9 |
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