BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa
Summary: Increasing plant resistance to Verticillium wilt (VW), which causes massive losses of Brassica rapa crops, is a challenge worldwide. However, few causal genes for VW resistance have been identified by forward genetic approaches, resulting in limited application in breeding. We combine a gen...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
|
| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221112472300949X |
| _version_ | 1797731122462851072 |
|---|---|
| author | Tongbing Su Weihong Wang Zheng Wang Peirong Li Xiaoyun Xin Yangjun Yu Deshuang Zhang Xiuyun Zhao Jiao Wang Liling Sun Guihua Jin Fenglan Zhang Shuancang Yu |
| author_facet | Tongbing Su Weihong Wang Zheng Wang Peirong Li Xiaoyun Xin Yangjun Yu Deshuang Zhang Xiuyun Zhao Jiao Wang Liling Sun Guihua Jin Fenglan Zhang Shuancang Yu |
| author_sort | Tongbing Su |
| collection | DOAJ |
| description | Summary: Increasing plant resistance to Verticillium wilt (VW), which causes massive losses of Brassica rapa crops, is a challenge worldwide. However, few causal genes for VW resistance have been identified by forward genetic approaches, resulting in limited application in breeding. We combine a genome-wide association study in a natural population and quantitative trait locus mapping in an F2 population and identify that the MYB transcription factor BrMYB108 regulates plant resistance to VW. A 179 bp insertion in the BrMYB108 promoter alters its expression pattern during Verticillium longisporum (VL) infection. High BrMYB108 expression leads to high VL resistance, which is confirmed by disease resistance tests using BrMYB108 overexpression and loss-of-function mutants. Furthermore, we verify that BrMYB108 confers VL resistance by regulating reactive oxygen species (ROS) generation through binding to the promoters of respiratory burst oxidase genes (Rboh). A loss-of-function mutant of AtRbohF in Arabidopsis shows significant susceptibility to VL. Thus, BrMYB108 and its target ROS genes could be used as targets for genetic engineering for VL resistance of B. rapa. |
| first_indexed | 2024-03-12T11:54:13Z |
| format | Article |
| id | doaj.art-ebf13d13341d4a7d8bb9f2781c42d7ed |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-12T11:54:13Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-ebf13d13341d4a7d8bb9f2781c42d7ed2023-08-31T05:02:15ZengElsevierCell Reports2211-12472023-08-01428112938BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapaTongbing Su0Weihong Wang1Zheng Wang2Peirong Li3Xiaoyun Xin4Yangjun Yu5Deshuang Zhang6Xiuyun Zhao7Jiao Wang8Liling Sun9Guihua Jin10Fenglan Zhang11Shuancang Yu12State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, ChinaState Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, ChinaState Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, ChinaState Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, ChinaState Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, ChinaState Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, ChinaState Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, ChinaState Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, ChinaState Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, ChinaState Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, ChinaState Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, ChinaState Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China; Corresponding authorState Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China; Corresponding authorSummary: Increasing plant resistance to Verticillium wilt (VW), which causes massive losses of Brassica rapa crops, is a challenge worldwide. However, few causal genes for VW resistance have been identified by forward genetic approaches, resulting in limited application in breeding. We combine a genome-wide association study in a natural population and quantitative trait locus mapping in an F2 population and identify that the MYB transcription factor BrMYB108 regulates plant resistance to VW. A 179 bp insertion in the BrMYB108 promoter alters its expression pattern during Verticillium longisporum (VL) infection. High BrMYB108 expression leads to high VL resistance, which is confirmed by disease resistance tests using BrMYB108 overexpression and loss-of-function mutants. Furthermore, we verify that BrMYB108 confers VL resistance by regulating reactive oxygen species (ROS) generation through binding to the promoters of respiratory burst oxidase genes (Rboh). A loss-of-function mutant of AtRbohF in Arabidopsis shows significant susceptibility to VL. Thus, BrMYB108 and its target ROS genes could be used as targets for genetic engineering for VL resistance of B. rapa.http://www.sciencedirect.com/science/article/pii/S221112472300949XCP: Plants |
| spellingShingle | Tongbing Su Weihong Wang Zheng Wang Peirong Li Xiaoyun Xin Yangjun Yu Deshuang Zhang Xiuyun Zhao Jiao Wang Liling Sun Guihua Jin Fenglan Zhang Shuancang Yu BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa Cell Reports CP: Plants |
| title | BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa |
| title_full | BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa |
| title_fullStr | BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa |
| title_full_unstemmed | BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa |
| title_short | BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa |
| title_sort | brmyb108 confers resistance to verticillium wilt by activating ros generation in brassica rapa |
| topic | CP: Plants |
| url | http://www.sciencedirect.com/science/article/pii/S221112472300949X |
| work_keys_str_mv | AT tongbingsu brmyb108confersresistancetoverticilliumwiltbyactivatingrosgenerationinbrassicarapa AT weihongwang brmyb108confersresistancetoverticilliumwiltbyactivatingrosgenerationinbrassicarapa AT zhengwang brmyb108confersresistancetoverticilliumwiltbyactivatingrosgenerationinbrassicarapa AT peirongli brmyb108confersresistancetoverticilliumwiltbyactivatingrosgenerationinbrassicarapa AT xiaoyunxin brmyb108confersresistancetoverticilliumwiltbyactivatingrosgenerationinbrassicarapa AT yangjunyu brmyb108confersresistancetoverticilliumwiltbyactivatingrosgenerationinbrassicarapa AT deshuangzhang brmyb108confersresistancetoverticilliumwiltbyactivatingrosgenerationinbrassicarapa AT xiuyunzhao brmyb108confersresistancetoverticilliumwiltbyactivatingrosgenerationinbrassicarapa AT jiaowang brmyb108confersresistancetoverticilliumwiltbyactivatingrosgenerationinbrassicarapa AT lilingsun brmyb108confersresistancetoverticilliumwiltbyactivatingrosgenerationinbrassicarapa AT guihuajin brmyb108confersresistancetoverticilliumwiltbyactivatingrosgenerationinbrassicarapa AT fenglanzhang brmyb108confersresistancetoverticilliumwiltbyactivatingrosgenerationinbrassicarapa AT shuancangyu brmyb108confersresistancetoverticilliumwiltbyactivatingrosgenerationinbrassicarapa |