Analysis of adult damselfly fecal material aids in the estimation of antibiotic-resistant Enterobacterales contamination of the local environment
Because damselflies are ubiquitously but focally present in natural environments and play a critical role as predators of other insect species, the fecal matter of damselflies may be useful for investigating antibiotic-resistant bacterial populations, including human pathogens, in local environments...
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| Format: | Article |
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PeerJ Inc.
2018-10-01
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| Online Access: | https://peerj.com/articles/5755.pdf |
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| author | Yuyu Yamaguchi Torahiko Okubo Mizue Matsushita Masashi Wataji Sumio Iwasaki Kasumi Hayasaka Kouzi Akizawa Junji Matsuo Chikara Shimizu Hiroyuki Yamaguchi |
| author_facet | Yuyu Yamaguchi Torahiko Okubo Mizue Matsushita Masashi Wataji Sumio Iwasaki Kasumi Hayasaka Kouzi Akizawa Junji Matsuo Chikara Shimizu Hiroyuki Yamaguchi |
| author_sort | Yuyu Yamaguchi |
| collection | DOAJ |
| description | Because damselflies are ubiquitously but focally present in natural environments and play a critical role as predators of other insect species, the fecal matter of damselflies may be useful for investigating antibiotic-resistant bacterial populations, including human pathogens, in local environments. We therefore examined the prevalence of antibiotic-resistant bacteria, including Enterobacterales, in fecal material from 383 damselflies (adults and larvae) collected from seven locations around Sapporo City, Japan, in 2016 and 2017. Fecal samples were plated on soybean casein digest (SCD) agar plates with and without antibiotics (SCD-A and SCD-w/o, respectively) to identify environmental bacteria and gut bacteria, respectively, and on MacConkey agar plates with antibiotics (MacConkey-A) to select for Gram-negative bacteria, including human pathogenic Enterobacterales species. The prevalence of colonies on each of the plates was compared, and representative colonies on MacConkey-A plates were identified to the species level using an API 20E kit and the MALDI Biotyper system. Overall, SCD-w/o plates showed a gut bacterial load of approximately 108 colony-forming units per adult damselfly or larva. There was a significant difference between the prevalence of colonies on the SCD-A and MacConkey-A plates, and a significantly increased prevalence of antibiotic-resistant bacteria on MacConkey-A plates was observed in samples collected from Shinoroshinkawa. Cluster analysis based on minimum inhibitory concentration values of 59 representative isolates from MacConkey-A agar plates revealed that samples from Shinoroshinkawa contained a higher prevalence of Enterobacterales than those from other sampling locations. Thus, fecal materials discharged by adult damselflies could be used in future studies as a simple tool for estimating antibiotic-resistant bacteria, including Enterobacterales species, in the local environment. |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:22:19Z |
| publishDate | 2018-10-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-ebf194433a724439b9f97e32ab93abc32023-12-03T11:34:50ZengPeerJ Inc.PeerJ2167-83592018-10-016e575510.7717/peerj.5755Analysis of adult damselfly fecal material aids in the estimation of antibiotic-resistant Enterobacterales contamination of the local environmentYuyu Yamaguchi0Torahiko Okubo1Mizue Matsushita2Masashi Wataji3Sumio Iwasaki4Kasumi Hayasaka5Kouzi Akizawa6Junji Matsuo7Chikara Shimizu8Hiroyuki Yamaguchi9Hokkaido Sapporo Asahigaoka Senior High School, Sapporo, JapanDepartment of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, JapanDepartment of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, JapanHokkaido Sapporo Asahigaoka Senior High School, Sapporo, JapanHokkaido University Hospital, Sapporo, JapanHokkaido University Hospital, Sapporo, JapanHokkaido University Hospital, Sapporo, JapanDepartment of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, JapanHokkaido University Hospital, Sapporo, JapanDepartment of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, JapanBecause damselflies are ubiquitously but focally present in natural environments and play a critical role as predators of other insect species, the fecal matter of damselflies may be useful for investigating antibiotic-resistant bacterial populations, including human pathogens, in local environments. We therefore examined the prevalence of antibiotic-resistant bacteria, including Enterobacterales, in fecal material from 383 damselflies (adults and larvae) collected from seven locations around Sapporo City, Japan, in 2016 and 2017. Fecal samples were plated on soybean casein digest (SCD) agar plates with and without antibiotics (SCD-A and SCD-w/o, respectively) to identify environmental bacteria and gut bacteria, respectively, and on MacConkey agar plates with antibiotics (MacConkey-A) to select for Gram-negative bacteria, including human pathogenic Enterobacterales species. The prevalence of colonies on each of the plates was compared, and representative colonies on MacConkey-A plates were identified to the species level using an API 20E kit and the MALDI Biotyper system. Overall, SCD-w/o plates showed a gut bacterial load of approximately 108 colony-forming units per adult damselfly or larva. There was a significant difference between the prevalence of colonies on the SCD-A and MacConkey-A plates, and a significantly increased prevalence of antibiotic-resistant bacteria on MacConkey-A plates was observed in samples collected from Shinoroshinkawa. Cluster analysis based on minimum inhibitory concentration values of 59 representative isolates from MacConkey-A agar plates revealed that samples from Shinoroshinkawa contained a higher prevalence of Enterobacterales than those from other sampling locations. Thus, fecal materials discharged by adult damselflies could be used in future studies as a simple tool for estimating antibiotic-resistant bacteria, including Enterobacterales species, in the local environment.https://peerj.com/articles/5755.pdfAntibiotic-resistant bacteriaFecal materialDamselflySapporoLocal environmentsEnterobacterales |
| spellingShingle | Yuyu Yamaguchi Torahiko Okubo Mizue Matsushita Masashi Wataji Sumio Iwasaki Kasumi Hayasaka Kouzi Akizawa Junji Matsuo Chikara Shimizu Hiroyuki Yamaguchi Analysis of adult damselfly fecal material aids in the estimation of antibiotic-resistant Enterobacterales contamination of the local environment PeerJ Antibiotic-resistant bacteria Fecal material Damselfly Sapporo Local environments Enterobacterales |
| title | Analysis of adult damselfly fecal material aids in the estimation of antibiotic-resistant Enterobacterales contamination of the local environment |
| title_full | Analysis of adult damselfly fecal material aids in the estimation of antibiotic-resistant Enterobacterales contamination of the local environment |
| title_fullStr | Analysis of adult damselfly fecal material aids in the estimation of antibiotic-resistant Enterobacterales contamination of the local environment |
| title_full_unstemmed | Analysis of adult damselfly fecal material aids in the estimation of antibiotic-resistant Enterobacterales contamination of the local environment |
| title_short | Analysis of adult damselfly fecal material aids in the estimation of antibiotic-resistant Enterobacterales contamination of the local environment |
| title_sort | analysis of adult damselfly fecal material aids in the estimation of antibiotic resistant enterobacterales contamination of the local environment |
| topic | Antibiotic-resistant bacteria Fecal material Damselfly Sapporo Local environments Enterobacterales |
| url | https://peerj.com/articles/5755.pdf |
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