Variability of Emaravirus Species Associated with Sterility Mosaic Disease of Pigeonpea in India Provides Evidence of Segment Reassortment
Sterility mosaic disease (SMD) of pigeonpea is a serious constraint for cultivation of pigeonpea in India and other South Asian countries. SMD of pigeonpea is associated with two distinct emaraviruses, Pigeonpea sterility mosaic virus 1 (PPSMV-1) and Pigeonpea sterility mosaic virus 2 (PPSMV-2), wit...
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MDPI AG
2017-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/9/7/183 |
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| author | Basavaprabhu L. Patil Meenakshi Dangwal Ritesh Mishra |
| author_facet | Basavaprabhu L. Patil Meenakshi Dangwal Ritesh Mishra |
| author_sort | Basavaprabhu L. Patil |
| collection | DOAJ |
| description | Sterility mosaic disease (SMD) of pigeonpea is a serious constraint for cultivation of pigeonpea in India and other South Asian countries. SMD of pigeonpea is associated with two distinct emaraviruses, Pigeonpea sterility mosaic virus 1 (PPSMV-1) and Pigeonpea sterility mosaic virus 2 (PPSMV-2), with genomes consisting of five and six negative-sense RNA segments, respectively. The recently published genome sequences of both PPSMV-1 and PPSMV-2 are from a single location, Patancheru from the state of Telangana in India. However, here we present the first report of sequence variability among 23 isolates of PPSMV-1 and PPSMV-2, collected from ten locations representing six states of India. Both PPSMV-1 and PPSMV-2 are shown to be present across India and to exhibit considerable sequence variability. Variability of RNA3 sequences was higher than the RNA4 sequences for both PPSMV-1 and PPSMV-2. Additionally, the sixth RNA segment (RNA6), previously reported to be associated with only PPSMV-2, is also associated with isolates of PPSMV-1. Multiplex reverse transcription PCR (RT-PCR) analyses show that PPSMV-1 and PPSMV-2 frequently occur as mixed infections. Further sequence analyses indicated the presence of reassortment of RNA4 between isolates of PPSMV-1 and PPSMV-2. |
| first_indexed | 2024-12-21T23:26:00Z |
| format | Article |
| id | doaj.art-ebf2b2c31151499d9aeaff73c6edd30a |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-21T23:26:00Z |
| publishDate | 2017-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-ebf2b2c31151499d9aeaff73c6edd30a2022-12-21T18:46:37ZengMDPI AGViruses1999-49152017-07-019718310.3390/v9070183v9070183Variability of Emaravirus Species Associated with Sterility Mosaic Disease of Pigeonpea in India Provides Evidence of Segment ReassortmentBasavaprabhu L. Patil0Meenakshi Dangwal1Ritesh Mishra2ICAR-National Research Centre on Plant Biotechnology, IARI, Pusa Campus, New Delhi 110012, IndiaICAR-National Research Centre on Plant Biotechnology, IARI, Pusa Campus, New Delhi 110012, IndiaICAR-National Research Centre on Plant Biotechnology, IARI, Pusa Campus, New Delhi 110012, IndiaSterility mosaic disease (SMD) of pigeonpea is a serious constraint for cultivation of pigeonpea in India and other South Asian countries. SMD of pigeonpea is associated with two distinct emaraviruses, Pigeonpea sterility mosaic virus 1 (PPSMV-1) and Pigeonpea sterility mosaic virus 2 (PPSMV-2), with genomes consisting of five and six negative-sense RNA segments, respectively. The recently published genome sequences of both PPSMV-1 and PPSMV-2 are from a single location, Patancheru from the state of Telangana in India. However, here we present the first report of sequence variability among 23 isolates of PPSMV-1 and PPSMV-2, collected from ten locations representing six states of India. Both PPSMV-1 and PPSMV-2 are shown to be present across India and to exhibit considerable sequence variability. Variability of RNA3 sequences was higher than the RNA4 sequences for both PPSMV-1 and PPSMV-2. Additionally, the sixth RNA segment (RNA6), previously reported to be associated with only PPSMV-2, is also associated with isolates of PPSMV-1. Multiplex reverse transcription PCR (RT-PCR) analyses show that PPSMV-1 and PPSMV-2 frequently occur as mixed infections. Further sequence analyses indicated the presence of reassortment of RNA4 between isolates of PPSMV-1 and PPSMV-2.https://www.mdpi.com/1999-4915/9/7/183pigeonpea sterility mosaic virusemaravirusvariabilityreassortment |
| spellingShingle | Basavaprabhu L. Patil Meenakshi Dangwal Ritesh Mishra Variability of Emaravirus Species Associated with Sterility Mosaic Disease of Pigeonpea in India Provides Evidence of Segment Reassortment Viruses pigeonpea sterility mosaic virus emaravirus variability reassortment |
| title | Variability of Emaravirus Species Associated with Sterility Mosaic Disease of Pigeonpea in India Provides Evidence of Segment Reassortment |
| title_full | Variability of Emaravirus Species Associated with Sterility Mosaic Disease of Pigeonpea in India Provides Evidence of Segment Reassortment |
| title_fullStr | Variability of Emaravirus Species Associated with Sterility Mosaic Disease of Pigeonpea in India Provides Evidence of Segment Reassortment |
| title_full_unstemmed | Variability of Emaravirus Species Associated with Sterility Mosaic Disease of Pigeonpea in India Provides Evidence of Segment Reassortment |
| title_short | Variability of Emaravirus Species Associated with Sterility Mosaic Disease of Pigeonpea in India Provides Evidence of Segment Reassortment |
| title_sort | variability of emaravirus species associated with sterility mosaic disease of pigeonpea in india provides evidence of segment reassortment |
| topic | pigeonpea sterility mosaic virus emaravirus variability reassortment |
| url | https://www.mdpi.com/1999-4915/9/7/183 |
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