Molecular and Chemical Markers to Illustrate the Complex Diversity of the Genus <i>Lupinus</i> (Fabaceae)
The potential of secondary metabolites as systematic markers to get new insights in an intricate phylogeny of a recent evolutionary radiation is explored. A chemosystematic study of the genus <i>Lupinus</i> (Fabaceae) was performed, using quinolizidine (QA) and piperidine alkaloids (ammo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Diversity |
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| Online Access: | https://www.mdpi.com/1424-2818/13/6/263 |
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| author | Kalina Bermúdez-Torres Maxime Ferval Arianna Michelle Hernández-Sánchez Andreas Tei Charles Gers Michael Wink Luc Legal |
| author_facet | Kalina Bermúdez-Torres Maxime Ferval Arianna Michelle Hernández-Sánchez Andreas Tei Charles Gers Michael Wink Luc Legal |
| author_sort | Kalina Bermúdez-Torres |
| collection | DOAJ |
| description | The potential of secondary metabolites as systematic markers to get new insights in an intricate phylogeny of a recent evolutionary radiation is explored. A chemosystematic study of the genus <i>Lupinus</i> (Fabaceae) was performed, using quinolizidine (QA) and piperidine alkaloids (ammodendrine) as diagnostic characters. Seven major QA and the piperidine alkaloid ammodendrine were found to be the most frequent compounds. Two groups were supported according to their geographic origin: an Old World/Atlantic American group and a West New World group and this pattern is concordant with molecular data (here, based on an original barcode approach using the nuclear marker ITS). However, QA profiles are less informative at the species level. Despite a lack of resolution within the two groups, the alkaloid profiles agree with well supported clades based on DNA molecular characters. The combined use of chemical and barcode genetic markers represents a viable alternative for separating recent evolutionary lineages to a first approximation without having to resort to an expensive and sophisticated molecular arsenal such as next generation sequencing. |
| first_indexed | 2024-03-10T10:30:47Z |
| format | Article |
| id | doaj.art-ebf3cd9e5a564dd6b5f2759d6f254727 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-10T10:30:47Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-ebf3cd9e5a564dd6b5f2759d6f2547272023-11-21T23:40:14ZengMDPI AGDiversity1424-28182021-06-0113626310.3390/d13060263Molecular and Chemical Markers to Illustrate the Complex Diversity of the Genus <i>Lupinus</i> (Fabaceae)Kalina Bermúdez-Torres0Maxime Ferval1Arianna Michelle Hernández-Sánchez2Andreas Tei3Charles Gers4Michael Wink5Luc Legal6Departamento de Biotecnología, Instituto Politécnico Nacional-Centro de Desarrollo de Productos Bióticos, Yautepec-Jojutla km 8, Calle CEPROBI No. 6 Col. San Isidro, Yautepec C.P. 62731, MexicoLaboratoire Ecologie Fonctionnelle et Environnement, Université Paul Sabatier, CNRS, 31062 Toulouse, FranceDepartamento de Biotecnología, Instituto Politécnico Nacional-Centro de Desarrollo de Productos Bióticos, Yautepec-Jojutla km 8, Calle CEPROBI No. 6 Col. San Isidro, Yautepec C.P. 62731, MexicoDepartment of Biology, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, INF 364, 69120 Heidelberg, GermanyLaboratoire Ecologie Fonctionnelle et Environnement, Université Paul Sabatier, CNRS, 31062 Toulouse, FranceDepartment of Biology, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, INF 364, 69120 Heidelberg, GermanyLaboratoire Ecologie Fonctionnelle et Environnement, Université Paul Sabatier, CNRS, 31062 Toulouse, FranceThe potential of secondary metabolites as systematic markers to get new insights in an intricate phylogeny of a recent evolutionary radiation is explored. A chemosystematic study of the genus <i>Lupinus</i> (Fabaceae) was performed, using quinolizidine (QA) and piperidine alkaloids (ammodendrine) as diagnostic characters. Seven major QA and the piperidine alkaloid ammodendrine were found to be the most frequent compounds. Two groups were supported according to their geographic origin: an Old World/Atlantic American group and a West New World group and this pattern is concordant with molecular data (here, based on an original barcode approach using the nuclear marker ITS). However, QA profiles are less informative at the species level. Despite a lack of resolution within the two groups, the alkaloid profiles agree with well supported clades based on DNA molecular characters. The combined use of chemical and barcode genetic markers represents a viable alternative for separating recent evolutionary lineages to a first approximation without having to resort to an expensive and sophisticated molecular arsenal such as next generation sequencing.https://www.mdpi.com/1424-2818/13/6/263alkaloidsITS barcodeFabaceaephylogenychemical diversity |
| spellingShingle | Kalina Bermúdez-Torres Maxime Ferval Arianna Michelle Hernández-Sánchez Andreas Tei Charles Gers Michael Wink Luc Legal Molecular and Chemical Markers to Illustrate the Complex Diversity of the Genus <i>Lupinus</i> (Fabaceae) Diversity alkaloids ITS barcode Fabaceae phylogeny chemical diversity |
| title | Molecular and Chemical Markers to Illustrate the Complex Diversity of the Genus <i>Lupinus</i> (Fabaceae) |
| title_full | Molecular and Chemical Markers to Illustrate the Complex Diversity of the Genus <i>Lupinus</i> (Fabaceae) |
| title_fullStr | Molecular and Chemical Markers to Illustrate the Complex Diversity of the Genus <i>Lupinus</i> (Fabaceae) |
| title_full_unstemmed | Molecular and Chemical Markers to Illustrate the Complex Diversity of the Genus <i>Lupinus</i> (Fabaceae) |
| title_short | Molecular and Chemical Markers to Illustrate the Complex Diversity of the Genus <i>Lupinus</i> (Fabaceae) |
| title_sort | molecular and chemical markers to illustrate the complex diversity of the genus i lupinus i fabaceae |
| topic | alkaloids ITS barcode Fabaceae phylogeny chemical diversity |
| url | https://www.mdpi.com/1424-2818/13/6/263 |
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