Chromatographic Detection of 8-Hydroxy-2′-Deoxyguanosine in Leukocytes of Asbestos Exposed Workers for Assessing Past and Recent Carcinogen Exposures
Asbestos fibers include a group of silicate minerals that occur in the environment and are widely employed in occupational settings. Asbestos exposure has been associated to various chronic diseases; such as pulmonary fibrosis; mesothelioma; and lung cancer; often characterized by a long period of l...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/10/4/239 |
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| author | Filippo Cellai Stefano Bonassi Alfonso Cristaudo Alessandra Bonotti Monica Neri Marcello Ceppi Marco Bruzzone Mirta Milić Armelle Munnia Marco Peluso |
| author_facet | Filippo Cellai Stefano Bonassi Alfonso Cristaudo Alessandra Bonotti Monica Neri Marcello Ceppi Marco Bruzzone Mirta Milić Armelle Munnia Marco Peluso |
| author_sort | Filippo Cellai |
| collection | DOAJ |
| description | Asbestos fibers include a group of silicate minerals that occur in the environment and are widely employed in occupational settings. Asbestos exposure has been associated to various chronic diseases; such as pulmonary fibrosis; mesothelioma; and lung cancer; often characterized by a long period of latency. Underlying mechanisms that are behind the carcinogenic effect of asbestos have not been fully clarified. Therefore; we have conducted an epidemiological study to evaluate the relationship between 8-hydroxy-2′-deoxyguanosine (8-oxodG), one of the most reliable biomarkers of oxidative stress and oxidative DNA damage; and asbestos exposure in the peripheral blood of residents in Tuscany and Liguria regions; Italy; stratified by occupational exposure to this carcinogen. Levels of 8-oxodG were expressed such as relative adduct labeling (RAL); the frequency of 8-oxodG per 10<sup>5</sup> deoxyguanosine was significantly higher among exposed workers with respect to the controls; i.e., 3.0 ± 0.2 Standard Error (SE) in asbestos workers versus a value of 1.3 ± 0.1 (SE) in unexposed controls (<i>p</i> < 0.001). When the relationship with occupational history was investigated; significant higher levels of 8-oxodG were measured in current and former asbestos workers vs. healthy controls; 3.1 ± 0.3 (SE) and 2.9 ± 0.2 (SE), respectively. After stratification for occupational history; a significant 194% excess of adducts was found in workers with 10 or more years of past asbestos exposure (<i>p</i> < 0.001). 8-oxodG can be used for medical surveillance programs of cohorts of workers with past and recent exposures to carcinogens for the identification of subjects requiring a more intense clinical surveillance. |
| first_indexed | 2024-03-10T20:20:47Z |
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| id | doaj.art-ebf9d62d9c4445e7bf4ce31a0e6eb174 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T20:20:47Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-ebf9d62d9c4445e7bf4ce31a0e6eb1742023-11-19T22:15:53ZengMDPI AGDiagnostics2075-44182020-04-0110423910.3390/diagnostics10040239Chromatographic Detection of 8-Hydroxy-2′-Deoxyguanosine in Leukocytes of Asbestos Exposed Workers for Assessing Past and Recent Carcinogen ExposuresFilippo Cellai0Stefano Bonassi1Alfonso Cristaudo2Alessandra Bonotti3Monica Neri4Marcello Ceppi5Marco Bruzzone6Mirta Milić7Armelle Munnia8Marco Peluso9Cancer Risk Factor Branch, Regional Cancer Prevention Laboratory, ISPRO-Study, Prevention and Oncology Network Institute, 50139 Florence, ItalyClinical and Molecular Epidemiology, IRCCS San Raffaele Pisana, 00166 Rome, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56010 Pisa, ItalyOccupational Medicine Unit, University of Pisa, 56010 Pisa, ItalyClinical and Molecular Epidemiology, IRCCS San Raffaele Pisana, 00166 Rome, ItalyUnit of Clinical Epidemiology, IRCCS Ospedale Policlinico San Martino, 16131 Genoa, ItalyUnit of Clinical Epidemiology, IRCCS Ospedale Policlinico San Martino, 16131 Genoa, ItalyMutagenesis Unit, Institute for Medical Research and Occupational Health, 10000 Zagreb, CroatiaCancer Risk Factor Branch, Regional Cancer Prevention Laboratory, ISPRO-Study, Prevention and Oncology Network Institute, 50139 Florence, ItalyCancer Risk Factor Branch, Regional Cancer Prevention Laboratory, ISPRO-Study, Prevention and Oncology Network Institute, 50139 Florence, ItalyAsbestos fibers include a group of silicate minerals that occur in the environment and are widely employed in occupational settings. Asbestos exposure has been associated to various chronic diseases; such as pulmonary fibrosis; mesothelioma; and lung cancer; often characterized by a long period of latency. Underlying mechanisms that are behind the carcinogenic effect of asbestos have not been fully clarified. Therefore; we have conducted an epidemiological study to evaluate the relationship between 8-hydroxy-2′-deoxyguanosine (8-oxodG), one of the most reliable biomarkers of oxidative stress and oxidative DNA damage; and asbestos exposure in the peripheral blood of residents in Tuscany and Liguria regions; Italy; stratified by occupational exposure to this carcinogen. Levels of 8-oxodG were expressed such as relative adduct labeling (RAL); the frequency of 8-oxodG per 10<sup>5</sup> deoxyguanosine was significantly higher among exposed workers with respect to the controls; i.e., 3.0 ± 0.2 Standard Error (SE) in asbestos workers versus a value of 1.3 ± 0.1 (SE) in unexposed controls (<i>p</i> < 0.001). When the relationship with occupational history was investigated; significant higher levels of 8-oxodG were measured in current and former asbestos workers vs. healthy controls; 3.1 ± 0.3 (SE) and 2.9 ± 0.2 (SE), respectively. After stratification for occupational history; a significant 194% excess of adducts was found in workers with 10 or more years of past asbestos exposure (<i>p</i> < 0.001). 8-oxodG can be used for medical surveillance programs of cohorts of workers with past and recent exposures to carcinogens for the identification of subjects requiring a more intense clinical surveillance.https://www.mdpi.com/2075-4418/10/4/239asbestosleukocytes8-oxodG |
| spellingShingle | Filippo Cellai Stefano Bonassi Alfonso Cristaudo Alessandra Bonotti Monica Neri Marcello Ceppi Marco Bruzzone Mirta Milić Armelle Munnia Marco Peluso Chromatographic Detection of 8-Hydroxy-2′-Deoxyguanosine in Leukocytes of Asbestos Exposed Workers for Assessing Past and Recent Carcinogen Exposures Diagnostics asbestos leukocytes 8-oxodG |
| title | Chromatographic Detection of 8-Hydroxy-2′-Deoxyguanosine in Leukocytes of Asbestos Exposed Workers for Assessing Past and Recent Carcinogen Exposures |
| title_full | Chromatographic Detection of 8-Hydroxy-2′-Deoxyguanosine in Leukocytes of Asbestos Exposed Workers for Assessing Past and Recent Carcinogen Exposures |
| title_fullStr | Chromatographic Detection of 8-Hydroxy-2′-Deoxyguanosine in Leukocytes of Asbestos Exposed Workers for Assessing Past and Recent Carcinogen Exposures |
| title_full_unstemmed | Chromatographic Detection of 8-Hydroxy-2′-Deoxyguanosine in Leukocytes of Asbestos Exposed Workers for Assessing Past and Recent Carcinogen Exposures |
| title_short | Chromatographic Detection of 8-Hydroxy-2′-Deoxyguanosine in Leukocytes of Asbestos Exposed Workers for Assessing Past and Recent Carcinogen Exposures |
| title_sort | chromatographic detection of 8 hydroxy 2 deoxyguanosine in leukocytes of asbestos exposed workers for assessing past and recent carcinogen exposures |
| topic | asbestos leukocytes 8-oxodG |
| url | https://www.mdpi.com/2075-4418/10/4/239 |
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