High dietary non-starch polysaccharides detrimental to nutrient digestibility, digestive enzyme activity, growth performance, and intestinal morphology in largemouth bass, Micropterus salmoides
An 8-weeks feeding trial was carried out to evaluate the effects of different levels of dietary non-starch polysaccharide on the growth, apparent nutrient digestibility, intestinal development, and morphology of largemouth bass (Micropterus salmoides). Seven isoproteic and isolipidic experimental di...
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1015371/full |
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| author | Yu Liu Yu Liu Yu Liu Jiongting Fan Jiongting Fan Jiongting Fan Huajing Huang Huajing Huang Huajing Huang Hang Zhou Hang Zhou Hang Zhou Yixiong Cao Yixiong Cao Yixiong Cao Yumeng Zhang Yumeng Zhang Yumeng Zhang Wen Jiang Wen Jiang Wen Jiang Wei Zhang Wei Zhang Wei Zhang Junming Deng Junming Deng Junming Deng Beiping Tan Beiping Tan Beiping Tan |
| author_facet | Yu Liu Yu Liu Yu Liu Jiongting Fan Jiongting Fan Jiongting Fan Huajing Huang Huajing Huang Huajing Huang Hang Zhou Hang Zhou Hang Zhou Yixiong Cao Yixiong Cao Yixiong Cao Yumeng Zhang Yumeng Zhang Yumeng Zhang Wen Jiang Wen Jiang Wen Jiang Wei Zhang Wei Zhang Wei Zhang Junming Deng Junming Deng Junming Deng Beiping Tan Beiping Tan Beiping Tan |
| author_sort | Yu Liu |
| collection | DOAJ |
| description | An 8-weeks feeding trial was carried out to evaluate the effects of different levels of dietary non-starch polysaccharide on the growth, apparent nutrient digestibility, intestinal development, and morphology of largemouth bass (Micropterus salmoides). Seven isoproteic and isolipidic experimental diets were formulated (crude protein 47.00%, crude lipid 12.50%), containing 0, 3, 6, 9, 12, 15, and 18% non-starch polysaccharides (NSPs) (named Control, NSPs3, NSPs6, NSPs9, NSPs12, NSPs15, and NSPs18), respectively. Dietary inclusion of NSPs below 9% showed no negative impacts on fish growth and feed utilization efficiency, whereas dietary NSPs inclusion level above 9% decreased weight gain rate, specific growth rate, protein efficiency, protein deposition rate, apparent digestibility of dry matter and protein, and were accompanied by a reduction in intestinal protease, Na+/K+-ATPase and alkaline phosphatase activity and an increase in feed intake and feed coefficient. The activity of lipase was significantly decreased when dietary inclusion of 15 and 18% NSPs. Moreover, the lipid deposition rate and the apparent digestibility of lipids were significantly decreased since dietary inclusion of 9% NSPs. Dietary inclusion of NSPs above 12% significantly up-regulated intestinal GLP-2 gene’s expression, and was accompanied by significant changes in hindgut morphology, including increases in villus length and width, muscularis thickness and number of goblet cell, as well as a decrease in crypt depth. Additionally, dietary inclusion of NSPs above 3% significantly increased intestinal length index, and the viserosomatic index was significantly increased when dietary NSPs exceeded 15%. The linear regression analysis based on weight gain rate and feed coefficient showed that the appropriate dietary NSPs level of juvenile largemouth bass should not above 5.51%. In conclusion, high dietary NSPs adversely affects digestive enzyme activity and intestinal morphology, which in turn reduced the apparent digestibility of dietary nutrients and growth of juvenile largemouth bass. |
| first_indexed | 2024-04-11T07:21:33Z |
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| last_indexed | 2024-04-11T07:21:33Z |
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| spelling | doaj.art-ebfeda726c0f4324a59e1de8d410ba132022-12-22T04:37:14ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-10-01910.3389/fnut.2022.10153711015371High dietary non-starch polysaccharides detrimental to nutrient digestibility, digestive enzyme activity, growth performance, and intestinal morphology in largemouth bass, Micropterus salmoidesYu Liu0Yu Liu1Yu Liu2Jiongting Fan3Jiongting Fan4Jiongting Fan5Huajing Huang6Huajing Huang7Huajing Huang8Hang Zhou9Hang Zhou10Hang Zhou11Yixiong Cao12Yixiong Cao13Yixiong Cao14Yumeng Zhang15Yumeng Zhang16Yumeng Zhang17Wen Jiang18Wen Jiang19Wen Jiang20Wei Zhang21Wei Zhang22Wei Zhang23Junming Deng24Junming Deng25Junming Deng26Beiping Tan27Beiping Tan28Beiping Tan29College of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaAquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, ChinaKey Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaAquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, ChinaKey Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaAquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, ChinaKey Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaAquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, ChinaKey Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaAquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, ChinaKey Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaAquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, ChinaKey Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaAquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, ChinaKey Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaAquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, ChinaKey Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaAquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, ChinaKey Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaAquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, ChinaKey Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaAn 8-weeks feeding trial was carried out to evaluate the effects of different levels of dietary non-starch polysaccharide on the growth, apparent nutrient digestibility, intestinal development, and morphology of largemouth bass (Micropterus salmoides). Seven isoproteic and isolipidic experimental diets were formulated (crude protein 47.00%, crude lipid 12.50%), containing 0, 3, 6, 9, 12, 15, and 18% non-starch polysaccharides (NSPs) (named Control, NSPs3, NSPs6, NSPs9, NSPs12, NSPs15, and NSPs18), respectively. Dietary inclusion of NSPs below 9% showed no negative impacts on fish growth and feed utilization efficiency, whereas dietary NSPs inclusion level above 9% decreased weight gain rate, specific growth rate, protein efficiency, protein deposition rate, apparent digestibility of dry matter and protein, and were accompanied by a reduction in intestinal protease, Na+/K+-ATPase and alkaline phosphatase activity and an increase in feed intake and feed coefficient. The activity of lipase was significantly decreased when dietary inclusion of 15 and 18% NSPs. Moreover, the lipid deposition rate and the apparent digestibility of lipids were significantly decreased since dietary inclusion of 9% NSPs. Dietary inclusion of NSPs above 12% significantly up-regulated intestinal GLP-2 gene’s expression, and was accompanied by significant changes in hindgut morphology, including increases in villus length and width, muscularis thickness and number of goblet cell, as well as a decrease in crypt depth. Additionally, dietary inclusion of NSPs above 3% significantly increased intestinal length index, and the viserosomatic index was significantly increased when dietary NSPs exceeded 15%. The linear regression analysis based on weight gain rate and feed coefficient showed that the appropriate dietary NSPs level of juvenile largemouth bass should not above 5.51%. In conclusion, high dietary NSPs adversely affects digestive enzyme activity and intestinal morphology, which in turn reduced the apparent digestibility of dietary nutrients and growth of juvenile largemouth bass.https://www.frontiersin.org/articles/10.3389/fnut.2022.1015371/fullnon-starch polysaccharideslargemouth bassgrowth performanceapparent nutrient digestibilityintestinal morphology |
| spellingShingle | Yu Liu Yu Liu Yu Liu Jiongting Fan Jiongting Fan Jiongting Fan Huajing Huang Huajing Huang Huajing Huang Hang Zhou Hang Zhou Hang Zhou Yixiong Cao Yixiong Cao Yixiong Cao Yumeng Zhang Yumeng Zhang Yumeng Zhang Wen Jiang Wen Jiang Wen Jiang Wei Zhang Wei Zhang Wei Zhang Junming Deng Junming Deng Junming Deng Beiping Tan Beiping Tan Beiping Tan High dietary non-starch polysaccharides detrimental to nutrient digestibility, digestive enzyme activity, growth performance, and intestinal morphology in largemouth bass, Micropterus salmoides Frontiers in Nutrition non-starch polysaccharides largemouth bass growth performance apparent nutrient digestibility intestinal morphology |
| title | High dietary non-starch polysaccharides detrimental to nutrient digestibility, digestive enzyme activity, growth performance, and intestinal morphology in largemouth bass, Micropterus salmoides |
| title_full | High dietary non-starch polysaccharides detrimental to nutrient digestibility, digestive enzyme activity, growth performance, and intestinal morphology in largemouth bass, Micropterus salmoides |
| title_fullStr | High dietary non-starch polysaccharides detrimental to nutrient digestibility, digestive enzyme activity, growth performance, and intestinal morphology in largemouth bass, Micropterus salmoides |
| title_full_unstemmed | High dietary non-starch polysaccharides detrimental to nutrient digestibility, digestive enzyme activity, growth performance, and intestinal morphology in largemouth bass, Micropterus salmoides |
| title_short | High dietary non-starch polysaccharides detrimental to nutrient digestibility, digestive enzyme activity, growth performance, and intestinal morphology in largemouth bass, Micropterus salmoides |
| title_sort | high dietary non starch polysaccharides detrimental to nutrient digestibility digestive enzyme activity growth performance and intestinal morphology in largemouth bass micropterus salmoides |
| topic | non-starch polysaccharides largemouth bass growth performance apparent nutrient digestibility intestinal morphology |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1015371/full |
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