Fungal microbiome related to mycotoxin contamination in medicinal and edible seed Semen Persicae
Medicinal and edible seed Semen Persicae is susceptible to mycotoxin and fungal contamination. However, the occurrence of mycotoxin contamination and fungal infection is still unclear. In this paper, ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry and high-thr...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023070044 |
| _version_ | 1827803126073131008 |
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| author | Guangfei Wei Bo Zhang Yichuan Liang Zhaoyu Zhang Conglian Liang Lan Wu Huatao Yu Yongqing Zhang Shilin Chen Linlin Dong |
| author_facet | Guangfei Wei Bo Zhang Yichuan Liang Zhaoyu Zhang Conglian Liang Lan Wu Huatao Yu Yongqing Zhang Shilin Chen Linlin Dong |
| author_sort | Guangfei Wei |
| collection | DOAJ |
| description | Medicinal and edible seed Semen Persicae is susceptible to mycotoxin and fungal contamination. However, the occurrence of mycotoxin contamination and fungal infection is still unclear. In this paper, ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry and high-throughput sequencing were conducted to determine the mycotoxin contents and fungal abundances of Semen Persicae. 42.86% of samples were positive for aflatoxin B1 (26.48–48.37 μg/kg) and 28.57% of samples were positive for aflatoxin B2 (1.47–4.82 μg/kg). Ochratoxin A and fumonisin B1 were only detected in one sample (91.02 and 34.61 μg/kg, respectively). Chao 1 and Shannon indices were significantly higher in the Dalian of Liaoning, Baotou of Innermongolia and Langfang of Hebei regions than in other groups. Ascomycota, Basidiomycota, Wallemia, Candica, Saccharomyces and Aspergillus were the predominant fungi and they were significantly region-specific. Simultaneously, the diversity, composition and co-occurrence network complexity in the mycotoxin-free group were significantly higher than those in the mycotoxin-contaminated group. Spearman correlation analysis showed aflatoxins, ochratoxin A and fumonisins contents were positively and significantly correlated with the abundances of Aspergillus, Rhodotorula, Wallemia and Candida. In conclusion, this study reported the prevalence of mycotoxin contamination and the great diversity of fungi associated with Semen Persicae for the first time, providing an early warning for subsequent potential mycotoxin biosynthesis. |
| first_indexed | 2024-03-11T20:50:05Z |
| format | Article |
| id | doaj.art-ec00e249844f4a5dae932c68220290ec |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-11T20:50:05Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-ec00e249844f4a5dae932c68220290ec2023-10-01T06:01:18ZengElsevierHeliyon2405-84402023-09-0199e19796Fungal microbiome related to mycotoxin contamination in medicinal and edible seed Semen PersicaeGuangfei Wei0Bo Zhang1Yichuan Liang2Zhaoyu Zhang3Conglian Liang4Lan Wu5Huatao Yu6Yongqing Zhang7Shilin Chen8Linlin Dong9Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, ChinaSchool of Pharmacy, Linyi University, Linyi, 276000, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China; Shandong University of Traditional Chinese Medicine, Jinan, 250355, ChinaShandong University of Traditional Chinese Medicine, Jinan, 250355, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, ChinaShandong University of Traditional Chinese Medicine, Jinan, 250355, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China; Corresponding author.Medicinal and edible seed Semen Persicae is susceptible to mycotoxin and fungal contamination. However, the occurrence of mycotoxin contamination and fungal infection is still unclear. In this paper, ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry and high-throughput sequencing were conducted to determine the mycotoxin contents and fungal abundances of Semen Persicae. 42.86% of samples were positive for aflatoxin B1 (26.48–48.37 μg/kg) and 28.57% of samples were positive for aflatoxin B2 (1.47–4.82 μg/kg). Ochratoxin A and fumonisin B1 were only detected in one sample (91.02 and 34.61 μg/kg, respectively). Chao 1 and Shannon indices were significantly higher in the Dalian of Liaoning, Baotou of Innermongolia and Langfang of Hebei regions than in other groups. Ascomycota, Basidiomycota, Wallemia, Candica, Saccharomyces and Aspergillus were the predominant fungi and they were significantly region-specific. Simultaneously, the diversity, composition and co-occurrence network complexity in the mycotoxin-free group were significantly higher than those in the mycotoxin-contaminated group. Spearman correlation analysis showed aflatoxins, ochratoxin A and fumonisins contents were positively and significantly correlated with the abundances of Aspergillus, Rhodotorula, Wallemia and Candida. In conclusion, this study reported the prevalence of mycotoxin contamination and the great diversity of fungi associated with Semen Persicae for the first time, providing an early warning for subsequent potential mycotoxin biosynthesis.http://www.sciencedirect.com/science/article/pii/S2405844023070044Semen persicaeMycotoxin contaminationFungal infectionUltra-high performance liquid chromatography-tandem triple quadrupole mass spectrometryHigh-throughput sequencing |
| spellingShingle | Guangfei Wei Bo Zhang Yichuan Liang Zhaoyu Zhang Conglian Liang Lan Wu Huatao Yu Yongqing Zhang Shilin Chen Linlin Dong Fungal microbiome related to mycotoxin contamination in medicinal and edible seed Semen Persicae Heliyon Semen persicae Mycotoxin contamination Fungal infection Ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry High-throughput sequencing |
| title | Fungal microbiome related to mycotoxin contamination in medicinal and edible seed Semen Persicae |
| title_full | Fungal microbiome related to mycotoxin contamination in medicinal and edible seed Semen Persicae |
| title_fullStr | Fungal microbiome related to mycotoxin contamination in medicinal and edible seed Semen Persicae |
| title_full_unstemmed | Fungal microbiome related to mycotoxin contamination in medicinal and edible seed Semen Persicae |
| title_short | Fungal microbiome related to mycotoxin contamination in medicinal and edible seed Semen Persicae |
| title_sort | fungal microbiome related to mycotoxin contamination in medicinal and edible seed semen persicae |
| topic | Semen persicae Mycotoxin contamination Fungal infection Ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry High-throughput sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023070044 |
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