Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy
Abstract In the past decade, extracellular vesicles (EVs) have attracted substantial interest in biomedicine. With progress in the field, we have an increasing understanding of cellular responses to EVs. In this Technical Report, we describe the direct nanoinjection of EVs into the cytoplasm of sing...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | Journal of Extracellular Vesicles |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/jev2.12388 |
| _version_ | 1827397677947551744 |
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| author | Kinga Dóra Kovács Tamás Visnovitz Tamás Gerecsei Beatrix Peter Sándor Kurunczi Anna Koncz Krisztina Németh Dorina Lenzinger Krisztina V. Vukman Anna Balogh Imola Rajmon Péter Lőrincz Inna Székács Edit I. Buzás Robert Horvath |
| author_facet | Kinga Dóra Kovács Tamás Visnovitz Tamás Gerecsei Beatrix Peter Sándor Kurunczi Anna Koncz Krisztina Németh Dorina Lenzinger Krisztina V. Vukman Anna Balogh Imola Rajmon Péter Lőrincz Inna Székács Edit I. Buzás Robert Horvath |
| author_sort | Kinga Dóra Kovács |
| collection | DOAJ |
| description | Abstract In the past decade, extracellular vesicles (EVs) have attracted substantial interest in biomedicine. With progress in the field, we have an increasing understanding of cellular responses to EVs. In this Technical Report, we describe the direct nanoinjection of EVs into the cytoplasm of single cells of different cell lines. By using robotic fluidic force microscopy (robotic FluidFM), nanoinjection of GFP positive EVs and EV‐like particles into single live HeLa, H9c2, MDA‐MB‐231 and LCLC‐103H cells proved to be feasible. This injection platform offered the advantage of high cell selectivity and efficiency. The nanoinjected EVs were initially localized in concentrated spot‐like regions within the cytoplasm. Later, they were transported towards the periphery of the cells. Based on our proof‐of‐principle data, robotic FluidFM is suitable for targeting single living cells by EVs and may lead to information about intracellular EV cargo delivery at a single‐cell level. |
| first_indexed | 2024-03-08T19:14:05Z |
| format | Article |
| id | doaj.art-ec0324f266c348fba555e10b43d9430e |
| institution | Directory Open Access Journal |
| issn | 2001-3078 |
| language | English |
| last_indexed | 2024-03-08T19:14:05Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Vesicles |
| spelling | doaj.art-ec0324f266c348fba555e10b43d9430e2023-12-27T09:15:41ZengWileyJournal of Extracellular Vesicles2001-30782023-12-011212n/an/a10.1002/jev2.12388Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopyKinga Dóra Kovács0Tamás Visnovitz1Tamás Gerecsei2Beatrix Peter3Sándor Kurunczi4Anna Koncz5Krisztina Németh6Dorina Lenzinger7Krisztina V. Vukman8Anna Balogh9Imola Rajmon10Péter Lőrincz11Inna Székács12Edit I. Buzás13Robert Horvath14Nanobiosensorics Laboratory Institute of Technical Physics and Materials Science, HUN‐REN Centre for Energy Research Budapest HungaryDepartment of Genetics, Cell‐ and Immunobiology Semmelweis University Budapest HungaryNanobiosensorics Laboratory Institute of Technical Physics and Materials Science, HUN‐REN Centre for Energy Research Budapest HungaryNanobiosensorics Laboratory Institute of Technical Physics and Materials Science, HUN‐REN Centre for Energy Research Budapest HungaryNanobiosensorics Laboratory Institute of Technical Physics and Materials Science, HUN‐REN Centre for Energy Research Budapest HungaryDepartment of Genetics, Cell‐ and Immunobiology Semmelweis University Budapest HungaryDepartment of Genetics, Cell‐ and Immunobiology Semmelweis University Budapest HungaryDepartment of Genetics, Cell‐ and Immunobiology Semmelweis University Budapest HungaryDepartment of Genetics, Cell‐ and Immunobiology Semmelweis University Budapest HungaryNanobiosensorics Laboratory Institute of Technical Physics and Materials Science, HUN‐REN Centre for Energy Research Budapest HungaryNanobiosensorics Laboratory Institute of Technical Physics and Materials Science, HUN‐REN Centre for Energy Research Budapest HungaryDepartment of Anatomy, Cell and Developmental Biology Eötvös Loránd University Budapest HungaryNanobiosensorics Laboratory Institute of Technical Physics and Materials Science, HUN‐REN Centre for Energy Research Budapest HungaryDepartment of Genetics, Cell‐ and Immunobiology Semmelweis University Budapest HungaryNanobiosensorics Laboratory Institute of Technical Physics and Materials Science, HUN‐REN Centre for Energy Research Budapest HungaryAbstract In the past decade, extracellular vesicles (EVs) have attracted substantial interest in biomedicine. With progress in the field, we have an increasing understanding of cellular responses to EVs. In this Technical Report, we describe the direct nanoinjection of EVs into the cytoplasm of single cells of different cell lines. By using robotic fluidic force microscopy (robotic FluidFM), nanoinjection of GFP positive EVs and EV‐like particles into single live HeLa, H9c2, MDA‐MB‐231 and LCLC‐103H cells proved to be feasible. This injection platform offered the advantage of high cell selectivity and efficiency. The nanoinjected EVs were initially localized in concentrated spot‐like regions within the cytoplasm. Later, they were transported towards the periphery of the cells. Based on our proof‐of‐principle data, robotic FluidFM is suitable for targeting single living cells by EVs and may lead to information about intracellular EV cargo delivery at a single‐cell level.https://doi.org/10.1002/jev2.12388cell‐to‐cell transportintracellular deliverymRNA injectionorganelle‐specific targetingrobotic FluidFMsingle‐cell |
| spellingShingle | Kinga Dóra Kovács Tamás Visnovitz Tamás Gerecsei Beatrix Peter Sándor Kurunczi Anna Koncz Krisztina Németh Dorina Lenzinger Krisztina V. Vukman Anna Balogh Imola Rajmon Péter Lőrincz Inna Székács Edit I. Buzás Robert Horvath Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy Journal of Extracellular Vesicles cell‐to‐cell transport intracellular delivery mRNA injection organelle‐specific targeting robotic FluidFM single‐cell |
| title | Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy |
| title_full | Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy |
| title_fullStr | Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy |
| title_full_unstemmed | Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy |
| title_short | Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy |
| title_sort | nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy |
| topic | cell‐to‐cell transport intracellular delivery mRNA injection organelle‐specific targeting robotic FluidFM single‐cell |
| url | https://doi.org/10.1002/jev2.12388 |
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