Milk metagenomics and cheese-making properties as affected by indoor farming and summer highland grazing
ABSTRACT: The study of the complex relationships between milk metagenomics and milk composition and cheese-making efficiency as affected by indoor farming and summer highland grazing was the aim of the present work. The experimental design considered monthly sampling (over 5 mo) of the milk produced...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | Journal of Dairy Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030222006695 |
| _version_ | 1811199644488695808 |
|---|---|
| author | Giorgia Secchi Nicolò Amalfitano Ilaria Carafa Elena Franciosi Luigi Gallo Stefano Schiavon Enrico Sturaro Franco Tagliapietra Giovanni Bittante |
| author_facet | Giorgia Secchi Nicolò Amalfitano Ilaria Carafa Elena Franciosi Luigi Gallo Stefano Schiavon Enrico Sturaro Franco Tagliapietra Giovanni Bittante |
| author_sort | Giorgia Secchi |
| collection | DOAJ |
| description | ABSTRACT: The study of the complex relationships between milk metagenomics and milk composition and cheese-making efficiency as affected by indoor farming and summer highland grazing was the aim of the present work. The experimental design considered monthly sampling (over 5 mo) of the milk produced by 12 Brown Swiss cows divided into 2 groups: the first remained on a lowland indoor farm from June to October, and the second was moved to highland pastures in July and then returned to the lowland farm in September. The resulting 60 milk samples (2 kg each) were used to analyze milk composition, milk coagulation, curd firming, and syneresis processes, and to make individual model cheeses to measure cheese yields and nutrient recoveries in the cheese. After DNA extraction and Illumina Miseq sequencing, milk microbiota amplicons were also processed by means of an open-source pipeline called Quantitative Insights Into Microbial Ecology (Qiime2, version 2018.2; https://qiime2.org). Out of a total of 44 taxa analyzed, 13 bacterial taxa were considered important for the dairy industry (lactic acid bacteria, LAB, 5 taxa; and spoilage bacteria, 4) and for human (other probiotics, 2) and animal health (pathogenic bacteria, 2). The results revealed the transhumant group of cows transferred to summer highland pastures showed an increase in almost all the LAB taxa, bifidobacteria, and propionibacteria, and a reduction in spoilage taxa. All the metagenomic changes disappeared when the transhumant cows were moved back to the permanent indoor farm. The relationships between 17 microbial traits and 30 compositional and technological milk traits were investigated through analysis of correlation and latent explanatory factor analysis. Eight latent factors were identified, explaining 75.3% of the total variance, 2 of which were mainly based on microbial traits: pro-dairy bacteria (14% of total variance, improving during summer pasturing) and pathogenic bacteria (6.0% of total variance). Some bacterial traits contributed to other compositional-technological latent factors (gelation, udder health, and caseins). |
| first_indexed | 2024-04-12T01:52:09Z |
| format | Article |
| id | doaj.art-ec036fbfe04e4518b37ad8c7f3983919 |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-04-12T01:52:09Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-ec036fbfe04e4518b37ad8c7f39839192022-12-22T03:52:55ZengElsevierJournal of Dairy Science0022-03022023-01-01106196116Milk metagenomics and cheese-making properties as affected by indoor farming and summer highland grazingGiorgia Secchi0Nicolò Amalfitano1Ilaria Carafa2Elena Franciosi3Luigi Gallo4Stefano Schiavon5Enrico Sturaro6Franco Tagliapietra7Giovanni Bittante8Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE) University of Padova (Padua), 35020 Legnaro (PD), Italy; Research and Innovation Centre, Edmund Mach Foundation, San Michele all'Adige, 38010 San Michele all'Adige (TN), ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE) University of Padova (Padua), 35020 Legnaro (PD), Italy; Corresponding authorResearch and Innovation Centre, Edmund Mach Foundation, San Michele all'Adige, 38010 San Michele all'Adige (TN), ItalyResearch and Innovation Centre, Edmund Mach Foundation, San Michele all'Adige, 38010 San Michele all'Adige (TN), ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE) University of Padova (Padua), 35020 Legnaro (PD), ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE) University of Padova (Padua), 35020 Legnaro (PD), ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE) University of Padova (Padua), 35020 Legnaro (PD), ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE) University of Padova (Padua), 35020 Legnaro (PD), ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE) University of Padova (Padua), 35020 Legnaro (PD), ItalyABSTRACT: The study of the complex relationships between milk metagenomics and milk composition and cheese-making efficiency as affected by indoor farming and summer highland grazing was the aim of the present work. The experimental design considered monthly sampling (over 5 mo) of the milk produced by 12 Brown Swiss cows divided into 2 groups: the first remained on a lowland indoor farm from June to October, and the second was moved to highland pastures in July and then returned to the lowland farm in September. The resulting 60 milk samples (2 kg each) were used to analyze milk composition, milk coagulation, curd firming, and syneresis processes, and to make individual model cheeses to measure cheese yields and nutrient recoveries in the cheese. After DNA extraction and Illumina Miseq sequencing, milk microbiota amplicons were also processed by means of an open-source pipeline called Quantitative Insights Into Microbial Ecology (Qiime2, version 2018.2; https://qiime2.org). Out of a total of 44 taxa analyzed, 13 bacterial taxa were considered important for the dairy industry (lactic acid bacteria, LAB, 5 taxa; and spoilage bacteria, 4) and for human (other probiotics, 2) and animal health (pathogenic bacteria, 2). The results revealed the transhumant group of cows transferred to summer highland pastures showed an increase in almost all the LAB taxa, bifidobacteria, and propionibacteria, and a reduction in spoilage taxa. All the metagenomic changes disappeared when the transhumant cows were moved back to the permanent indoor farm. The relationships between 17 microbial traits and 30 compositional and technological milk traits were investigated through analysis of correlation and latent explanatory factor analysis. Eight latent factors were identified, explaining 75.3% of the total variance, 2 of which were mainly based on microbial traits: pro-dairy bacteria (14% of total variance, improving during summer pasturing) and pathogenic bacteria (6.0% of total variance). Some bacterial traits contributed to other compositional-technological latent factors (gelation, udder health, and caseins).http://www.sciencedirect.com/science/article/pii/S0022030222006695milk microbiotaprobiotic bacteriadairy bacteriamilk spoilagesummer transhumance |
| spellingShingle | Giorgia Secchi Nicolò Amalfitano Ilaria Carafa Elena Franciosi Luigi Gallo Stefano Schiavon Enrico Sturaro Franco Tagliapietra Giovanni Bittante Milk metagenomics and cheese-making properties as affected by indoor farming and summer highland grazing Journal of Dairy Science milk microbiota probiotic bacteria dairy bacteria milk spoilage summer transhumance |
| title | Milk metagenomics and cheese-making properties as affected by indoor farming and summer highland grazing |
| title_full | Milk metagenomics and cheese-making properties as affected by indoor farming and summer highland grazing |
| title_fullStr | Milk metagenomics and cheese-making properties as affected by indoor farming and summer highland grazing |
| title_full_unstemmed | Milk metagenomics and cheese-making properties as affected by indoor farming and summer highland grazing |
| title_short | Milk metagenomics and cheese-making properties as affected by indoor farming and summer highland grazing |
| title_sort | milk metagenomics and cheese making properties as affected by indoor farming and summer highland grazing |
| topic | milk microbiota probiotic bacteria dairy bacteria milk spoilage summer transhumance |
| url | http://www.sciencedirect.com/science/article/pii/S0022030222006695 |
| work_keys_str_mv | AT giorgiasecchi milkmetagenomicsandcheesemakingpropertiesasaffectedbyindoorfarmingandsummerhighlandgrazing AT nicoloamalfitano milkmetagenomicsandcheesemakingpropertiesasaffectedbyindoorfarmingandsummerhighlandgrazing AT ilariacarafa milkmetagenomicsandcheesemakingpropertiesasaffectedbyindoorfarmingandsummerhighlandgrazing AT elenafranciosi milkmetagenomicsandcheesemakingpropertiesasaffectedbyindoorfarmingandsummerhighlandgrazing AT luigigallo milkmetagenomicsandcheesemakingpropertiesasaffectedbyindoorfarmingandsummerhighlandgrazing AT stefanoschiavon milkmetagenomicsandcheesemakingpropertiesasaffectedbyindoorfarmingandsummerhighlandgrazing AT enricosturaro milkmetagenomicsandcheesemakingpropertiesasaffectedbyindoorfarmingandsummerhighlandgrazing AT francotagliapietra milkmetagenomicsandcheesemakingpropertiesasaffectedbyindoorfarmingandsummerhighlandgrazing AT giovannibittante milkmetagenomicsandcheesemakingpropertiesasaffectedbyindoorfarmingandsummerhighlandgrazing |