Optimizing pentose utilization in yeast: the need for novel tools and approaches
<p>Abstract</p> <p>Hexose and pentose cofermentation is regarded as one of the chief obstacles impeding economical conversion of lignocellulosic biomass to biofuels. Over time, successful application of traditional metabolic engineering strategy has produced yeast strains capable o...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-11-01
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| Series: | Biotechnology for Biofuels |
| Online Access: | http://www.biotechnologyforbiofuels.com/content/3/1/24 |
| _version_ | 1811243802046758912 |
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| author | Lee Sun-Mi Young Eric Alper Hal |
| author_facet | Lee Sun-Mi Young Eric Alper Hal |
| author_sort | Lee Sun-Mi |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Hexose and pentose cofermentation is regarded as one of the chief obstacles impeding economical conversion of lignocellulosic biomass to biofuels. Over time, successful application of traditional metabolic engineering strategy has produced yeast strains capable of utilizing the pentose sugars (especially xylose and arabinose) as sole carbon sources, yet major difficulties still remain for engineering simultaneous, exogenous sugar metabolism. Beyond catabolic pathways, the focus must shift towards non-traditional aspects of cellular engineering such as host molecular transport capability, catabolite sensing and stress response mechanisms. This review highlights the need for an approach termed 'panmetabolic engineering', a new paradigm for integrating new carbon sources into host metabolic pathways. This approach will concurrently optimize the interdependent processes of transport and metabolism using novel combinatorial techniques and global cellular engineering. As a result, panmetabolic engineering is a whole pathway approach emphasizing better pathways, reduced glucose-induced repression and increased product tolerance. In this paper, recent publications are reviewed in light of this approach and their potential to expand metabolic engineering tools. Collectively, traditional approaches and panmetabolic engineering enable the reprogramming of extant biological complexity and incorporation of exogenous carbon catabolism.</p> |
| first_indexed | 2024-04-12T14:14:47Z |
| format | Article |
| id | doaj.art-ec046260983e49c2a6f20a8bed916d65 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-12T14:14:47Z |
| publishDate | 2010-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-ec046260983e49c2a6f20a8bed916d652022-12-22T03:29:47ZengBMCBiotechnology for Biofuels1754-68342010-11-01312410.1186/1754-6834-3-24Optimizing pentose utilization in yeast: the need for novel tools and approachesLee Sun-MiYoung EricAlper Hal<p>Abstract</p> <p>Hexose and pentose cofermentation is regarded as one of the chief obstacles impeding economical conversion of lignocellulosic biomass to biofuels. Over time, successful application of traditional metabolic engineering strategy has produced yeast strains capable of utilizing the pentose sugars (especially xylose and arabinose) as sole carbon sources, yet major difficulties still remain for engineering simultaneous, exogenous sugar metabolism. Beyond catabolic pathways, the focus must shift towards non-traditional aspects of cellular engineering such as host molecular transport capability, catabolite sensing and stress response mechanisms. This review highlights the need for an approach termed 'panmetabolic engineering', a new paradigm for integrating new carbon sources into host metabolic pathways. This approach will concurrently optimize the interdependent processes of transport and metabolism using novel combinatorial techniques and global cellular engineering. As a result, panmetabolic engineering is a whole pathway approach emphasizing better pathways, reduced glucose-induced repression and increased product tolerance. In this paper, recent publications are reviewed in light of this approach and their potential to expand metabolic engineering tools. Collectively, traditional approaches and panmetabolic engineering enable the reprogramming of extant biological complexity and incorporation of exogenous carbon catabolism.</p>http://www.biotechnologyforbiofuels.com/content/3/1/24 |
| spellingShingle | Lee Sun-Mi Young Eric Alper Hal Optimizing pentose utilization in yeast: the need for novel tools and approaches Biotechnology for Biofuels |
| title | Optimizing pentose utilization in yeast: the need for novel tools and approaches |
| title_full | Optimizing pentose utilization in yeast: the need for novel tools and approaches |
| title_fullStr | Optimizing pentose utilization in yeast: the need for novel tools and approaches |
| title_full_unstemmed | Optimizing pentose utilization in yeast: the need for novel tools and approaches |
| title_short | Optimizing pentose utilization in yeast: the need for novel tools and approaches |
| title_sort | optimizing pentose utilization in yeast the need for novel tools and approaches |
| url | http://www.biotechnologyforbiofuels.com/content/3/1/24 |
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