Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isol...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Centers for Disease Control and Prevention
2018-03-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/24/3/17-0459_article |
| _version_ | 1818042669584089088 |
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| author | Viktor Månsson Janet R. Gilsdorf Gunnar Kahlmeter Mogens Kilian J. Simon Kroll Kristian Riesbeck Fredrik Resman |
| author_facet | Viktor Månsson Janet R. Gilsdorf Gunnar Kahlmeter Mogens Kilian J. Simon Kroll Kristian Riesbeck Fredrik Resman |
| author_sort | Viktor Månsson |
| collection | DOAJ |
| description | Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a−f and nontypeable; n = 258) and isogenic capsule transformants (types a−d) were investigated. Principal component and biomarker analyses of mass spectra showed clustering, and mass peaks correlated with capsule type-specific genetic lineages. We used 31 selected isolates to construct a capsule typing database. Validation with the remaining isolates (n = 227) showed 100% sensitivity and 92.2% specificity for encapsulated strains (a−f; n = 61). Blinded validation of a supplemented database (n = 50) using clinical isolates (n = 126) showed 100% sensitivity and 100% specificity for encapsulated strains (b, e, and f; n = 28). MALDI-TOF mass spectrometry is an accurate method for capsule typing of H. influenzae. |
| first_indexed | 2024-12-10T08:50:00Z |
| format | Article |
| id | doaj.art-ec06ac42ee4a48c48da006c4c3d3fb98 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-10T08:50:00Z |
| publishDate | 2018-03-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-ec06ac42ee4a48c48da006c4c3d3fb982022-12-22T01:55:37ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592018-03-0124344345210.3201/eid2403.170459Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass SpectrometryViktor MånssonJanet R. GilsdorfGunnar KahlmeterMogens KilianJ. Simon KrollKristian RiesbeckFredrik ResmanEncapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a−f and nontypeable; n = 258) and isogenic capsule transformants (types a−d) were investigated. Principal component and biomarker analyses of mass spectra showed clustering, and mass peaks correlated with capsule type-specific genetic lineages. We used 31 selected isolates to construct a capsule typing database. Validation with the remaining isolates (n = 227) showed 100% sensitivity and 92.2% specificity for encapsulated strains (a−f; n = 61). Blinded validation of a supplemented database (n = 50) using clinical isolates (n = 126) showed 100% sensitivity and 100% specificity for encapsulated strains (b, e, and f; n = 28). MALDI-TOF mass spectrometry is an accurate method for capsule typing of H. influenzae.https://wwwnc.cdc.gov/eid/article/24/3/17-0459_articlebacterial typingserotypingbacterial capsulescapsule typingHaemophilus influenzaebacteria |
| spellingShingle | Viktor Månsson Janet R. Gilsdorf Gunnar Kahlmeter Mogens Kilian J. Simon Kroll Kristian Riesbeck Fredrik Resman Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Emerging Infectious Diseases bacterial typing serotyping bacterial capsules capsule typing Haemophilus influenzae bacteria |
| title | Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
| title_full | Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
| title_fullStr | Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
| title_full_unstemmed | Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
| title_short | Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
| title_sort | capsule typing of haemophilus influenzae by matrix assisted laser desorption ionization time of flight mass spectrometry |
| topic | bacterial typing serotyping bacterial capsules capsule typing Haemophilus influenzae bacteria |
| url | https://wwwnc.cdc.gov/eid/article/24/3/17-0459_article |
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