Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses
Autohemotherapy (AHT) is a procedure in which blood is collected by venipuncture, followed by immediate reinjection to activate an animal’s immune system. In this preliminary study, we evaluated the effect of AHT on clinical, hematological and biochemical parameters and immune responses in horses. E...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade de Medicina Veterinária do Estado do Rio de Janeiro
2021-06-01
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| Series: | Brazilian Journal of Veterinary Medicine |
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| Online Access: | https://rbmv.org/BJVM/article/view/1187 |
| _version_ | 1818939490567192576 |
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| author | Paula Rodrigues Lopes Mauricio Azevedo Batista Rodrigo Pedro Soares Jéssica Guerra de Oliveira Fabiola de Oliveira Paes Leme Olindo Assis Martins-Filho Renata de Pino Albuquerque Maranhão Raphael Rocha Wenceslau Maristela Silveira Palhares Marcio Sobreira Silva Araújo |
| author_facet | Paula Rodrigues Lopes Mauricio Azevedo Batista Rodrigo Pedro Soares Jéssica Guerra de Oliveira Fabiola de Oliveira Paes Leme Olindo Assis Martins-Filho Renata de Pino Albuquerque Maranhão Raphael Rocha Wenceslau Maristela Silveira Palhares Marcio Sobreira Silva Araújo |
| author_sort | Paula Rodrigues Lopes |
| collection | DOAJ |
| description | Autohemotherapy (AHT) is a procedure in which blood is collected by venipuncture, followed by immediate reinjection to activate an animal’s immune system. In this preliminary study, we evaluated the effect of AHT on clinical, hematological and biochemical parameters and immune responses in horses. Eight healthy animals (four males and four females) were clinically evaluated and subjected to AHT (three applications at a weekly interval). Blood samples were collected before AHT (T0) and 3 d after each procedure (T1, T2, and T3). AHT did not affect most clinical, biochemical, and hematological parameters in the test animals. However, flow cytometry detected an increase in T lymphocytes (CD3+) and cytokine production (IFN-γ, IL-10, IL-4, and IL-6). In addition, AHT stimulated phagocytosis by neutrophils at T3. Although preliminary, our results suggest that AHT is a promising procedure for equine management, as AHT was found to stimulate the horses’ immune responses without affecting their general status. |
| first_indexed | 2024-12-20T06:24:35Z |
| format | Article |
| id | doaj.art-ec07c94de72c4bb3976d151442c5c407 |
| institution | Directory Open Access Journal |
| issn | 0100-2430 2527-2179 |
| language | English |
| last_indexed | 2024-12-20T06:24:35Z |
| publishDate | 2021-06-01 |
| publisher | Sociedade de Medicina Veterinária do Estado do Rio de Janeiro |
| record_format | Article |
| series | Brazilian Journal of Veterinary Medicine |
| spelling | doaj.art-ec07c94de72c4bb3976d151442c5c4072022-12-21T19:50:21ZengSociedade de Medicina Veterinária do Estado do Rio de JaneiroBrazilian Journal of Veterinary Medicine0100-24302527-21792021-06-01431e000821e00082110.29374/2527-2179.bjvm0008211057Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horsesPaula Rodrigues Lopes0Mauricio Azevedo Batista1Rodrigo Pedro Soares2https://orcid.org/0000-0002-7966-3629Jéssica Guerra de Oliveira3https://orcid.org/0000-0002-2783-7631Fabiola de Oliveira Paes Leme4https://orcid.org/0000-0002-4013-0147Olindo Assis Martins-Filho5https://orcid.org/0000-0002-5494-4889Renata de Pino Albuquerque Maranhão6https://orcid.org/0000-0002-2084-672XRaphael Rocha Wenceslau7Maristela Silveira Palhares8Marcio Sobreira Silva Araújo9https://orcid.org/0000-0002-7532-3360Veterinarian, Laboratório de Ensino Pesquisa e Extensão em equídeos de tração (LEPET), Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil. Biologist, DSc., Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil. Biologist, DSc., Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil.Veterinarian, LEPET, Escola de Veterinária, UFMG, Belo Horizonte, MG, Brazil. Veterinarian, DSc., Laboratório de Análises Clínicas, Escola de Veterinária, UFMG, Belo Horizonte, MG, Brazil.Biologist, DSc., Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil.Veterinarian, DSc., LEPET, Escola de Veterinária, UFMG, Belo Horizonte, MG, Brazil. Veterinarian, DSc., Departamento de Clínica e Cirurgia veterinárias, Escola de Veterinária, UFMG, Belo Horizonte, MG, BrazilVeterinarian, DSc., LEPET, Escola de Veterinária, UFMG, Belo Horizonte, MG, Brazil. Biologist, DSc., Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil.Autohemotherapy (AHT) is a procedure in which blood is collected by venipuncture, followed by immediate reinjection to activate an animal’s immune system. In this preliminary study, we evaluated the effect of AHT on clinical, hematological and biochemical parameters and immune responses in horses. Eight healthy animals (four males and four females) were clinically evaluated and subjected to AHT (three applications at a weekly interval). Blood samples were collected before AHT (T0) and 3 d after each procedure (T1, T2, and T3). AHT did not affect most clinical, biochemical, and hematological parameters in the test animals. However, flow cytometry detected an increase in T lymphocytes (CD3+) and cytokine production (IFN-γ, IL-10, IL-4, and IL-6). In addition, AHT stimulated phagocytosis by neutrophils at T3. Although preliminary, our results suggest that AHT is a promising procedure for equine management, as AHT was found to stimulate the horses’ immune responses without affecting their general status.https://rbmv.org/BJVM/article/view/1187autohemotherapy, horse, cytokines, phagocytosis. |
| spellingShingle | Paula Rodrigues Lopes Mauricio Azevedo Batista Rodrigo Pedro Soares Jéssica Guerra de Oliveira Fabiola de Oliveira Paes Leme Olindo Assis Martins-Filho Renata de Pino Albuquerque Maranhão Raphael Rocha Wenceslau Maristela Silveira Palhares Marcio Sobreira Silva Araújo Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses Brazilian Journal of Veterinary Medicine autohemotherapy, horse, cytokines, phagocytosis. |
| title | Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses |
| title_full | Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses |
| title_fullStr | Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses |
| title_full_unstemmed | Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses |
| title_short | Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses |
| title_sort | autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses |
| topic | autohemotherapy, horse, cytokines, phagocytosis. |
| url | https://rbmv.org/BJVM/article/view/1187 |
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