The effects of resveratrol feeding and exercise training on the skeletal muscle function and transcriptome of aged rats
This study investigated the effects of resveratrol feeding and exercise training on the skeletal muscle function and transcriptome of aged rats. Male SD rats (25 months old) were divided into the control group (Old), the daily exercise training group (Trained), and the resveratrol feeding group (Res...
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PeerJ Inc.
2019-07-01
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| Online Access: | https://peerj.com/articles/7199.pdf |
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| author | Jing Zhou Zhiyin Liao Jia Jia Jin-Liang Chen Qian Xiao |
| author_facet | Jing Zhou Zhiyin Liao Jia Jia Jin-Liang Chen Qian Xiao |
| author_sort | Jing Zhou |
| collection | DOAJ |
| description | This study investigated the effects of resveratrol feeding and exercise training on the skeletal muscle function and transcriptome of aged rats. Male SD rats (25 months old) were divided into the control group (Old), the daily exercise training group (Trained), and the resveratrol feeding group (Resveratrol). After 6 weeks of intervention, the body mass, grip strength, and gastrocnemius muscle mass were determined, and the muscle samples were analyzed by transcriptome sequencing. The differentially expressed genes were analyzed followed by GO enrichment analysis and KEGG analysis. The Old group showed positive increases in body mass, while both the Trained and Resveratrol groups showed negative growth. No significant differences in the gastrocnemius muscle index and absolute grip strength were found among the three groups. However, the relative grip strength was higher in the Trained group than in the Old group. Only 21 differentially expressed genes were identified in the Trained group vs. the Old group, and 12 differentially expressed genes were identified in the Resveratrol group vs. the Old group. The most enriched GO terms in the Trained group vs. the Old group were mainly associated with RNA metabolic processes and transmembrane transporters, and the significantly upregulated KEGG pathways included mucin-type O-glycan biosynthesis, drug metabolism, and pyrimidine metabolism. The most enriched GO terms in the Resveratrol group vs. the Old group were primarily associated with neurotransmitter transport and synaptic vesicle, and the upregulated KEGG pathways included synaptic vesicle cycle, nicotine addiction, retinol metabolism, insulin secretion, retrograde endocannabinoid signaling, and glutamatergic synapse. Neither exercise training nor resveratrol feeding has a notable effect on skeletal muscle function and related gene expression in aged rats. However, both exercise training and resveratrol feeding have strong effects on weight loss, which is beneficial for reducing the exercise loads of the elderly. |
| first_indexed | 2024-03-09T07:59:08Z |
| format | Article |
| id | doaj.art-ec0979ed50c642559f1986ae18d5a068 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:59:08Z |
| publishDate | 2019-07-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-ec0979ed50c642559f1986ae18d5a0682023-12-03T00:50:52ZengPeerJ Inc.PeerJ2167-83592019-07-017e719910.7717/peerj.7199The effects of resveratrol feeding and exercise training on the skeletal muscle function and transcriptome of aged ratsJing Zhou0Zhiyin Liao1Jia Jia2Jin-Liang Chen3Qian Xiao4Chongqing Medical and Pharmaceutical College, Chongqing, ChinaDepartment of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Medical and Pharmaceutical College, Chongqing, ChinaDepartment of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaThis study investigated the effects of resveratrol feeding and exercise training on the skeletal muscle function and transcriptome of aged rats. Male SD rats (25 months old) were divided into the control group (Old), the daily exercise training group (Trained), and the resveratrol feeding group (Resveratrol). After 6 weeks of intervention, the body mass, grip strength, and gastrocnemius muscle mass were determined, and the muscle samples were analyzed by transcriptome sequencing. The differentially expressed genes were analyzed followed by GO enrichment analysis and KEGG analysis. The Old group showed positive increases in body mass, while both the Trained and Resveratrol groups showed negative growth. No significant differences in the gastrocnemius muscle index and absolute grip strength were found among the three groups. However, the relative grip strength was higher in the Trained group than in the Old group. Only 21 differentially expressed genes were identified in the Trained group vs. the Old group, and 12 differentially expressed genes were identified in the Resveratrol group vs. the Old group. The most enriched GO terms in the Trained group vs. the Old group were mainly associated with RNA metabolic processes and transmembrane transporters, and the significantly upregulated KEGG pathways included mucin-type O-glycan biosynthesis, drug metabolism, and pyrimidine metabolism. The most enriched GO terms in the Resveratrol group vs. the Old group were primarily associated with neurotransmitter transport and synaptic vesicle, and the upregulated KEGG pathways included synaptic vesicle cycle, nicotine addiction, retinol metabolism, insulin secretion, retrograde endocannabinoid signaling, and glutamatergic synapse. Neither exercise training nor resveratrol feeding has a notable effect on skeletal muscle function and related gene expression in aged rats. However, both exercise training and resveratrol feeding have strong effects on weight loss, which is beneficial for reducing the exercise loads of the elderly.https://peerj.com/articles/7199.pdfExerciseResveratrolTranscriptomeMuscleSarcopenia |
| spellingShingle | Jing Zhou Zhiyin Liao Jia Jia Jin-Liang Chen Qian Xiao The effects of resveratrol feeding and exercise training on the skeletal muscle function and transcriptome of aged rats PeerJ Exercise Resveratrol Transcriptome Muscle Sarcopenia |
| title | The effects of resveratrol feeding and exercise training on the skeletal muscle function and transcriptome of aged rats |
| title_full | The effects of resveratrol feeding and exercise training on the skeletal muscle function and transcriptome of aged rats |
| title_fullStr | The effects of resveratrol feeding and exercise training on the skeletal muscle function and transcriptome of aged rats |
| title_full_unstemmed | The effects of resveratrol feeding and exercise training on the skeletal muscle function and transcriptome of aged rats |
| title_short | The effects of resveratrol feeding and exercise training on the skeletal muscle function and transcriptome of aged rats |
| title_sort | effects of resveratrol feeding and exercise training on the skeletal muscle function and transcriptome of aged rats |
| topic | Exercise Resveratrol Transcriptome Muscle Sarcopenia |
| url | https://peerj.com/articles/7199.pdf |
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