ARGONAUTE10 and ARGONAUTE1 regulate the termination of floral stem cells through two microRNAs in Arabidopsis.
Stem cells are crucial in morphogenesis in plants and animals. Much is known about the mechanisms that maintain stem cell fates or trigger their terminal differentiation. However, little is known about how developmental time impacts stem cell fates. Using Arabidopsis floral stem cells as a model, we...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-03-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3069122?pdf=render |
| _version_ | 1817982857186902016 |
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| author | Lijuan Ji Xigang Liu Jun Yan Wenming Wang Rae Eden Yumul Yun Ju Kim Thanh Theresa Dinh Jun Liu Xia Cui Binglian Zheng Manu Agarwal Chunyan Liu Xiaofeng Cao Guiliang Tang Xuemei Chen |
| author_facet | Lijuan Ji Xigang Liu Jun Yan Wenming Wang Rae Eden Yumul Yun Ju Kim Thanh Theresa Dinh Jun Liu Xia Cui Binglian Zheng Manu Agarwal Chunyan Liu Xiaofeng Cao Guiliang Tang Xuemei Chen |
| author_sort | Lijuan Ji |
| collection | DOAJ |
| description | Stem cells are crucial in morphogenesis in plants and animals. Much is known about the mechanisms that maintain stem cell fates or trigger their terminal differentiation. However, little is known about how developmental time impacts stem cell fates. Using Arabidopsis floral stem cells as a model, we show that stem cells can undergo precise temporal regulation governed by mechanisms that are distinct from, but integrated with, those that specify cell fates. We show that two microRNAs, miR172 and miR165/166, through targeting APETALA2 and type III homeodomain-leucine zipper (HD-Zip) genes, respectively, regulate the temporal program of floral stem cells. In particular, we reveal a role of the type III HD-Zip genes, previously known to specify lateral organ polarity, in stem cell termination. Both reduction in HD-Zip expression by over-expression of miR165/166 and mis-expression of HD-Zip genes by rendering them resistant to miR165/166 lead to prolonged floral stem cell activity, indicating that the expression of HD-Zip genes needs to be precisely controlled to achieve floral stem cell termination. We also show that both the ubiquitously expressed ARGONAUTE1 (AGO1) gene and its homolog AGO10, which exhibits highly restricted spatial expression patterns, are required to maintain the correct temporal program of floral stem cells. We provide evidence that AGO10, like AGO1, associates with miR172 and miR165/166 in vivo and exhibits "slicer" activity in vitro. Despite the common biological functions and similar biochemical activities, AGO1 and AGO10 exert different effects on miR165/166 in vivo. This work establishes a network of microRNAs and transcription factors governing the temporal program of floral stem cells and sheds light on the relationships among different AGO genes, which tend to exist in gene families in multicellular organisms. |
| first_indexed | 2024-04-13T23:26:34Z |
| format | Article |
| id | doaj.art-ec0a8b01522640caa92066041f43e52f |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-13T23:26:34Z |
| publishDate | 2011-03-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS Genetics |
| spelling | doaj.art-ec0a8b01522640caa92066041f43e52f2022-12-22T02:25:03ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042011-03-0173e100135810.1371/journal.pgen.1001358ARGONAUTE10 and ARGONAUTE1 regulate the termination of floral stem cells through two microRNAs in Arabidopsis.Lijuan JiXigang LiuJun YanWenming WangRae Eden YumulYun Ju KimThanh Theresa DinhJun LiuXia CuiBinglian ZhengManu AgarwalChunyan LiuXiaofeng CaoGuiliang TangXuemei ChenStem cells are crucial in morphogenesis in plants and animals. Much is known about the mechanisms that maintain stem cell fates or trigger their terminal differentiation. However, little is known about how developmental time impacts stem cell fates. Using Arabidopsis floral stem cells as a model, we show that stem cells can undergo precise temporal regulation governed by mechanisms that are distinct from, but integrated with, those that specify cell fates. We show that two microRNAs, miR172 and miR165/166, through targeting APETALA2 and type III homeodomain-leucine zipper (HD-Zip) genes, respectively, regulate the temporal program of floral stem cells. In particular, we reveal a role of the type III HD-Zip genes, previously known to specify lateral organ polarity, in stem cell termination. Both reduction in HD-Zip expression by over-expression of miR165/166 and mis-expression of HD-Zip genes by rendering them resistant to miR165/166 lead to prolonged floral stem cell activity, indicating that the expression of HD-Zip genes needs to be precisely controlled to achieve floral stem cell termination. We also show that both the ubiquitously expressed ARGONAUTE1 (AGO1) gene and its homolog AGO10, which exhibits highly restricted spatial expression patterns, are required to maintain the correct temporal program of floral stem cells. We provide evidence that AGO10, like AGO1, associates with miR172 and miR165/166 in vivo and exhibits "slicer" activity in vitro. Despite the common biological functions and similar biochemical activities, AGO1 and AGO10 exert different effects on miR165/166 in vivo. This work establishes a network of microRNAs and transcription factors governing the temporal program of floral stem cells and sheds light on the relationships among different AGO genes, which tend to exist in gene families in multicellular organisms.http://europepmc.org/articles/PMC3069122?pdf=render |
| spellingShingle | Lijuan Ji Xigang Liu Jun Yan Wenming Wang Rae Eden Yumul Yun Ju Kim Thanh Theresa Dinh Jun Liu Xia Cui Binglian Zheng Manu Agarwal Chunyan Liu Xiaofeng Cao Guiliang Tang Xuemei Chen ARGONAUTE10 and ARGONAUTE1 regulate the termination of floral stem cells through two microRNAs in Arabidopsis. PLoS Genetics |
| title | ARGONAUTE10 and ARGONAUTE1 regulate the termination of floral stem cells through two microRNAs in Arabidopsis. |
| title_full | ARGONAUTE10 and ARGONAUTE1 regulate the termination of floral stem cells through two microRNAs in Arabidopsis. |
| title_fullStr | ARGONAUTE10 and ARGONAUTE1 regulate the termination of floral stem cells through two microRNAs in Arabidopsis. |
| title_full_unstemmed | ARGONAUTE10 and ARGONAUTE1 regulate the termination of floral stem cells through two microRNAs in Arabidopsis. |
| title_short | ARGONAUTE10 and ARGONAUTE1 regulate the termination of floral stem cells through two microRNAs in Arabidopsis. |
| title_sort | argonaute10 and argonaute1 regulate the termination of floral stem cells through two micrornas in arabidopsis |
| url | http://europepmc.org/articles/PMC3069122?pdf=render |
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