Macroporous bioceramic scaffolds based on tricalcium phosphates reinforced with silica: microstructural, mechanical, and biological evaluation
The positive effect of silica on microstructural, mechanical and biological properties of calcium phosphate scaffolds was investigated in this study. Scaffolds containing 3D interconnected spherical macropores with diameters in the range of 300–770 µm were prepared by the polymer replica technique....
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-04-01
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| Series: | Journal of Asian Ceramic Societies |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21870764.2022.2053278 |
| _version_ | 1811222575731179520 |
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| author | Lenka Novotna Zdenek Chlup Josef Jaros Klara Castkova Daniel Drdlik Jakub Pospisil Ales Hampl Irena Koutna Jaroslav Cihlar |
| author_facet | Lenka Novotna Zdenek Chlup Josef Jaros Klara Castkova Daniel Drdlik Jakub Pospisil Ales Hampl Irena Koutna Jaroslav Cihlar |
| author_sort | Lenka Novotna |
| collection | DOAJ |
| description | The positive effect of silica on microstructural, mechanical and biological properties of calcium phosphate scaffolds was investigated in this study. Scaffolds containing 3D interconnected spherical macropores with diameters in the range of 300–770 µm were prepared by the polymer replica technique. Reinforcement was achieved by incorporating 5 to 20 wt % of colloidal silica into the initial hydroxyapatite (HA) powder. The HA was fully decomposed into alpha and beta-tricalcium phosphate, and silica was transformed into cristobalite at 1200°C. Silica reinforced scaffolds exhibited compressive strength in the range of 0.3 to 30 MPa at the total porosity of 98–40%. At a nominal porosity of 75%, the compressive strength was doubled compared to scaffolds without silica. When immersed into a cultivation medium, the formation of an apatite layer on the surfaces of scaffolds indicated their bioactivity. The supportive effect of the silicon enriched scaffolds was examined using three different types of cells (human adipose-derived stromal cells, L929, and ARPE-19 cells). The cells firmly adhered to the surfaces of composite scaffolds with no sign of induced cell death. Scaffolds were non-cytotoxic and had good biocompatibility in vitro. They are promising candidates for therapeutic applications in regenerative medicine. |
| first_indexed | 2024-04-12T08:18:14Z |
| format | Article |
| id | doaj.art-ec0b96f10eca444a986adb28fc9e7773 |
| institution | Directory Open Access Journal |
| issn | 2187-0764 |
| language | English |
| last_indexed | 2024-04-12T08:18:14Z |
| publishDate | 2022-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Asian Ceramic Societies |
| spelling | doaj.art-ec0b96f10eca444a986adb28fc9e77732022-12-22T03:40:42ZengTaylor & Francis GroupJournal of Asian Ceramic Societies2187-07642022-04-0110235636910.1080/21870764.2022.2053278Macroporous bioceramic scaffolds based on tricalcium phosphates reinforced with silica: microstructural, mechanical, and biological evaluationLenka Novotna0Zdenek Chlup1Josef Jaros2Klara Castkova3Daniel Drdlik4Jakub Pospisil5Ales Hampl6Irena Koutna7Jaroslav Cihlar8CEITEC - Central European Institute of Technology, Brno University of Technology, Brno, Czech RepublicCEITEC - IPM, Academy of Sciences of the Czech Republic, Brno, Czech RepublicFaculty of Medicine, Department of Histology and Embryology, Masaryk University, Brno, Czech RepublicCEITEC - Central European Institute of Technology, Brno University of Technology, Brno, Czech RepublicCEITEC - Central European Institute of Technology, Brno University of Technology, Brno, Czech RepublicFaculty of Medicine, Department of Histology and Embryology, Masaryk University, Brno, Czech RepublicFaculty of Medicine, Department of Histology and Embryology, Masaryk University, Brno, Czech RepublicFaculty of Medicine, Department of Histology and Embryology, Masaryk University, Brno, Czech RepublicCEITEC - Central European Institute of Technology, Brno University of Technology, Brno, Czech RepublicThe positive effect of silica on microstructural, mechanical and biological properties of calcium phosphate scaffolds was investigated in this study. Scaffolds containing 3D interconnected spherical macropores with diameters in the range of 300–770 µm were prepared by the polymer replica technique. Reinforcement was achieved by incorporating 5 to 20 wt % of colloidal silica into the initial hydroxyapatite (HA) powder. The HA was fully decomposed into alpha and beta-tricalcium phosphate, and silica was transformed into cristobalite at 1200°C. Silica reinforced scaffolds exhibited compressive strength in the range of 0.3 to 30 MPa at the total porosity of 98–40%. At a nominal porosity of 75%, the compressive strength was doubled compared to scaffolds without silica. When immersed into a cultivation medium, the formation of an apatite layer on the surfaces of scaffolds indicated their bioactivity. The supportive effect of the silicon enriched scaffolds was examined using three different types of cells (human adipose-derived stromal cells, L929, and ARPE-19 cells). The cells firmly adhered to the surfaces of composite scaffolds with no sign of induced cell death. Scaffolds were non-cytotoxic and had good biocompatibility in vitro. They are promising candidates for therapeutic applications in regenerative medicine.https://www.tandfonline.com/doi/10.1080/21870764.2022.2053278Bioceramicsscaffoldcalcium phosphatesilicacompressive strength |
| spellingShingle | Lenka Novotna Zdenek Chlup Josef Jaros Klara Castkova Daniel Drdlik Jakub Pospisil Ales Hampl Irena Koutna Jaroslav Cihlar Macroporous bioceramic scaffolds based on tricalcium phosphates reinforced with silica: microstructural, mechanical, and biological evaluation Journal of Asian Ceramic Societies Bioceramics scaffold calcium phosphate silica compressive strength |
| title | Macroporous bioceramic scaffolds based on tricalcium phosphates reinforced with silica: microstructural, mechanical, and biological evaluation |
| title_full | Macroporous bioceramic scaffolds based on tricalcium phosphates reinforced with silica: microstructural, mechanical, and biological evaluation |
| title_fullStr | Macroporous bioceramic scaffolds based on tricalcium phosphates reinforced with silica: microstructural, mechanical, and biological evaluation |
| title_full_unstemmed | Macroporous bioceramic scaffolds based on tricalcium phosphates reinforced with silica: microstructural, mechanical, and biological evaluation |
| title_short | Macroporous bioceramic scaffolds based on tricalcium phosphates reinforced with silica: microstructural, mechanical, and biological evaluation |
| title_sort | macroporous bioceramic scaffolds based on tricalcium phosphates reinforced with silica microstructural mechanical and biological evaluation |
| topic | Bioceramics scaffold calcium phosphate silica compressive strength |
| url | https://www.tandfonline.com/doi/10.1080/21870764.2022.2053278 |
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