Asymmetry of cell division in CFSE-based lymphocyte proliferation analysis
Flow cytometry-based analysis of lymphocyte division using carboxyfluorescein diacetate succinimidyl ester (CFSE) dye dilution permits acquisition of data describing cellular proliferation and differentiation. For example, CFSE histogram data enable quantitative insight into cellular turnover rates...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2013-09-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00264/full |
| _version_ | 1818031727855009792 |
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| author | Gennady eBocharov Tatyana eLuzyanina Jovana eCupovic Burkhard eLudewig |
| author_facet | Gennady eBocharov Tatyana eLuzyanina Jovana eCupovic Burkhard eLudewig |
| author_sort | Gennady eBocharov |
| collection | DOAJ |
| description | Flow cytometry-based analysis of lymphocyte division using carboxyfluorescein diacetate succinimidyl ester (CFSE) dye dilution permits acquisition of data describing cellular proliferation and differentiation. For example, CFSE histogram data enable quantitative insight into cellular turnover rates by applying mathematical models and parameter estimation techniques. Several mathematical models have been developed using different types of deterministic or stochastic approaches. However, analysis of CFSE proliferation assays is based on the premise that the label is halved in the two daughter cells. Importantly, asymmetry of protein distribution in lymphocyte division is a basic biological feature of cell division with the degree of the asymmetry depending on various factors. Here, we review the recent literature on asymmetric lymphocyte division and CFSE-based lymphocyte proliferation analysis. We suggest that division- and label-structured mathematical models describing CFSE-based cell proliferation should take into account asymmetry and time-lag in cell proliferation. Utilization of improved modeling algorithms will permit straightforward quantification of essential parameters describing the performance of activated lymphocytes. |
| first_indexed | 2024-12-10T05:56:05Z |
| format | Article |
| id | doaj.art-ec0ca8af85e24588935d0b4fa65e1985 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-10T05:56:05Z |
| publishDate | 2013-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-ec0ca8af85e24588935d0b4fa65e19852022-12-22T01:59:56ZengFrontiers Media S.A.Frontiers in Immunology1664-32242013-09-01410.3389/fimmu.2013.0026458023Asymmetry of cell division in CFSE-based lymphocyte proliferation analysisGennady eBocharov0Tatyana eLuzyanina1Jovana eCupovic2Burkhard eLudewig3Institute of Numerical Mathematics, Russian Academy of SciencesInstitute of Mathematical Problems in Biology, Russian Academy of SciencesInstitute of Immunobiology, Cantonal Hospital St. GallenInstitute of Immunobiology, Cantonal Hospital St. GallenFlow cytometry-based analysis of lymphocyte division using carboxyfluorescein diacetate succinimidyl ester (CFSE) dye dilution permits acquisition of data describing cellular proliferation and differentiation. For example, CFSE histogram data enable quantitative insight into cellular turnover rates by applying mathematical models and parameter estimation techniques. Several mathematical models have been developed using different types of deterministic or stochastic approaches. However, analysis of CFSE proliferation assays is based on the premise that the label is halved in the two daughter cells. Importantly, asymmetry of protein distribution in lymphocyte division is a basic biological feature of cell division with the degree of the asymmetry depending on various factors. Here, we review the recent literature on asymmetric lymphocyte division and CFSE-based lymphocyte proliferation analysis. We suggest that division- and label-structured mathematical models describing CFSE-based cell proliferation should take into account asymmetry and time-lag in cell proliferation. Utilization of improved modeling algorithms will permit straightforward quantification of essential parameters describing the performance of activated lymphocytes.http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00264/fullT cellsmathematical modelingparameter estimationCFSE assayasymmetric division |
| spellingShingle | Gennady eBocharov Tatyana eLuzyanina Jovana eCupovic Burkhard eLudewig Asymmetry of cell division in CFSE-based lymphocyte proliferation analysis Frontiers in Immunology T cells mathematical modeling parameter estimation CFSE assay asymmetric division |
| title | Asymmetry of cell division in CFSE-based lymphocyte proliferation analysis |
| title_full | Asymmetry of cell division in CFSE-based lymphocyte proliferation analysis |
| title_fullStr | Asymmetry of cell division in CFSE-based lymphocyte proliferation analysis |
| title_full_unstemmed | Asymmetry of cell division in CFSE-based lymphocyte proliferation analysis |
| title_short | Asymmetry of cell division in CFSE-based lymphocyte proliferation analysis |
| title_sort | asymmetry of cell division in cfse based lymphocyte proliferation analysis |
| topic | T cells mathematical modeling parameter estimation CFSE assay asymmetric division |
| url | http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00264/full |
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