Isolation and identification of eurycomanone from akar pasak bumi (Eurycoma longifolia, Jack) and its antiangiogenic activity
Pasak bumi is one of the Indonesian original plants having anti cancer activity. One compound of akar pasak bumi, i.e eurycomanone was proven to have cytotoxic effect on cancer cell culture by apoptosis induction on HeLa cells. Therefore, this study was aimed to isolate and identify euycomanone from...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2009-09-01
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| Series: | Indonesian Journal of Pharmacy |
| Subjects: | |
| Online Access: | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/502 |
| _version_ | 1818013730794897408 |
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| author | Sugiyanto Nina Salamah Mae Sri Hartati Farida Hayati, Pinus Jumariyatno Pinus Jumariyatno |
| author_facet | Sugiyanto Nina Salamah Mae Sri Hartati Farida Hayati, Pinus Jumariyatno Pinus Jumariyatno |
| author_sort | Sugiyanto |
| collection | DOAJ |
| description | Pasak bumi is one of the Indonesian original plants having anti cancer activity. One compound of akar pasak bumi, i.e eurycomanone was proven to have cytotoxic effect on cancer cell culture by apoptosis induction on HeLa cells. Therefore, this study was aimed to isolate and identify euycomanone from akar pasak bumi and to look at the anti angiogenic activity of the compounds on corio alantois membrane (CAM) of chicken embryo induced by bFGF.
The akar pasak bumi was ground and was macerated with methanol. The macerate was fractioned by mean of VLC. Six fractions was obtained. The second fraction exhibited two spots on a TLC system, one spot was cochromatographed with eurycomanone as the reference standart and another spot exhibited blue fluorecence at 254 nm. This second fraction, then, was chromatographed on a preparative scale TLC to separate isolate A. Anti angiogenic test was performed for Isolate A at concentration series of 5, 10, 15, 20, 40 μg/mL respectively. Egg embrio (8-9 days) were divided into 8 groups. The first group was implanted paper disc only, the second group were given bFGF on Tris HCl buffer and the third group were given bFGF (on Tris HCl buffer) + 0,8 % DMSO. The fourth to eighth group were induced by 10 ng bFGF and the test preparation at the respective concentration. After incubation at 39 °C for 3 days, each egg was opened, the content of each was aspired and discarded, the CAM sticked to its shell was examined macroscopically and microscopically for the formation of new blood vessels.
Based on spectral data of UV, IR, 1H NMR, 13C NMR and MS of isolate A and comparison with the spectral data of the standart, the isolate A was eurycomanone. Eurycomanone inhibited angiogenesis at concentration of 10 μg/mL. |
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| format | Article |
| id | doaj.art-ec0f0b9613dd407297816ebfd91d8bb5 |
| institution | Directory Open Access Journal |
| issn | 2338-9427 2338-9486 |
| language | English |
| last_indexed | 2024-04-14T06:36:55Z |
| publishDate | 2009-09-01 |
| publisher | Universitas Gadjah Mada |
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| series | Indonesian Journal of Pharmacy |
| spelling | doaj.art-ec0f0b9613dd407297816ebfd91d8bb52022-12-22T02:07:26ZengUniversitas Gadjah MadaIndonesian Journal of Pharmacy2338-94272338-94862009-09-0120311812610.14499/indonesianjpharm0iss0pp118-126Isolation and identification of eurycomanone from akar pasak bumi (Eurycoma longifolia, Jack) and its antiangiogenic activitySugiyanto 0Nina Salamah1Mae Sri Hartati2Farida Hayati, Pinus Jumariyatno3Pinus Jumariyatno4 Fakultas Farmasi Universitas Gadjah Mada, Fakultas Farmasi Universitas Gadjah Mada, Fakultas Kedokteran Universitas Gadjah Mada, Progdi Farmasi Universitas Islam Indonesia Progdi Farmasi Universitas Islam Indonesia Pasak bumi is one of the Indonesian original plants having anti cancer activity. One compound of akar pasak bumi, i.e eurycomanone was proven to have cytotoxic effect on cancer cell culture by apoptosis induction on HeLa cells. Therefore, this study was aimed to isolate and identify euycomanone from akar pasak bumi and to look at the anti angiogenic activity of the compounds on corio alantois membrane (CAM) of chicken embryo induced by bFGF. The akar pasak bumi was ground and was macerated with methanol. The macerate was fractioned by mean of VLC. Six fractions was obtained. The second fraction exhibited two spots on a TLC system, one spot was cochromatographed with eurycomanone as the reference standart and another spot exhibited blue fluorecence at 254 nm. This second fraction, then, was chromatographed on a preparative scale TLC to separate isolate A. Anti angiogenic test was performed for Isolate A at concentration series of 5, 10, 15, 20, 40 μg/mL respectively. Egg embrio (8-9 days) were divided into 8 groups. The first group was implanted paper disc only, the second group were given bFGF on Tris HCl buffer and the third group were given bFGF (on Tris HCl buffer) + 0,8 % DMSO. The fourth to eighth group were induced by 10 ng bFGF and the test preparation at the respective concentration. After incubation at 39 °C for 3 days, each egg was opened, the content of each was aspired and discarded, the CAM sticked to its shell was examined macroscopically and microscopically for the formation of new blood vessels. Based on spectral data of UV, IR, 1H NMR, 13C NMR and MS of isolate A and comparison with the spectral data of the standart, the isolate A was eurycomanone. Eurycomanone inhibited angiogenesis at concentration of 10 μg/mL.http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/502antiangiogenicangiogenesisE.longifoliaeurycomanoneCAM. |
| spellingShingle | Sugiyanto Nina Salamah Mae Sri Hartati Farida Hayati, Pinus Jumariyatno Pinus Jumariyatno Isolation and identification of eurycomanone from akar pasak bumi (Eurycoma longifolia, Jack) and its antiangiogenic activity Indonesian Journal of Pharmacy antiangiogenic angiogenesis E.longifolia eurycomanone CAM. |
| title | Isolation and identification of eurycomanone from akar pasak bumi (Eurycoma longifolia, Jack) and its antiangiogenic activity |
| title_full | Isolation and identification of eurycomanone from akar pasak bumi (Eurycoma longifolia, Jack) and its antiangiogenic activity |
| title_fullStr | Isolation and identification of eurycomanone from akar pasak bumi (Eurycoma longifolia, Jack) and its antiangiogenic activity |
| title_full_unstemmed | Isolation and identification of eurycomanone from akar pasak bumi (Eurycoma longifolia, Jack) and its antiangiogenic activity |
| title_short | Isolation and identification of eurycomanone from akar pasak bumi (Eurycoma longifolia, Jack) and its antiangiogenic activity |
| title_sort | isolation and identification of eurycomanone from akar pasak bumi eurycoma longifolia jack and its antiangiogenic activity |
| topic | antiangiogenic angiogenesis E.longifolia eurycomanone CAM. |
| url | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/502 |
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