Extraction and characterization of gelatin derived from acetic acid-treated black soldier fly larvae
Gelatin is a low-cost and widely used soluble protein compound derived from collagen. Black soldier fly (Hermetia illucens) larvae (BSFL) are a potential alternative source of gelatin due to their high nutritional value. This study investigated the effect of acetic acid pretreatment concentration, t...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Food Chemistry Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X2300103X |
| _version_ | 1827917367464689664 |
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| author | Lee-Kee Chua Pek-Kui Lim Yin-Yin Thoo Yun-Ping Neo Thuan-Chew Tan |
| author_facet | Lee-Kee Chua Pek-Kui Lim Yin-Yin Thoo Yun-Ping Neo Thuan-Chew Tan |
| author_sort | Lee-Kee Chua |
| collection | DOAJ |
| description | Gelatin is a low-cost and widely used soluble protein compound derived from collagen. Black soldier fly (Hermetia illucens) larvae (BSFL) are a potential alternative source of gelatin due to their high nutritional value. This study investigated the effect of acetic acid pretreatment concentration, time, and centrifugation speed on the yield of BSFL gelatin and characterized its physicochemical properties. BSFL gelatin extracted with 0.05 mol/L acetic acid for 3 h and centrifuged at 3000 × g (BSFL3) had the highest yield of 1.085%±0.2%. The BSFL3 gelatin had lower protein content and amino acids than commercial porcine gelatin, specifically proline and hydroxyproline. It also had a high melting temperature of 135.12±1.04 °C, viscosity of 1.69±0.05 mPa·s, and rough microstructure with low foaming properties. The BSFL gelatin is high in K (680/01 mg/kg) and contains less Na, Mg, Ca, and P. Field emission scanning electron microscopy (FE-SEM) and energy dispersive X-ray (EDX) revealed that BSFL3 gelatin has high potassium content. The results suggest that BSFL gelatin can be used as a novel techno-functional ingredient in the food industry. |
| first_indexed | 2024-03-13T03:27:49Z |
| format | Article |
| id | doaj.art-ec103c144c26457ab9500115ac6d1ec1 |
| institution | Directory Open Access Journal |
| issn | 2772-753X |
| language | English |
| last_indexed | 2024-03-13T03:27:49Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry Advances |
| spelling | doaj.art-ec103c144c26457ab9500115ac6d1ec12023-06-25T04:44:54ZengElsevierFood Chemistry Advances2772-753X2023-10-012100282Extraction and characterization of gelatin derived from acetic acid-treated black soldier fly larvaeLee-Kee Chua0Pek-Kui Lim1Yin-Yin Thoo2Yun-Ping Neo3Thuan-Chew Tan4School of Food Studies and Gastronomy, Taylor's University, Subang Jaya, Selangor 47500, Malaysia; School of Biosciences, Taylor's University, Subang Jaya, Selangor 47500, MalaysiaSchool of Food Studies and Gastronomy, Taylor's University, Subang Jaya, Selangor 47500, Malaysia; Corresponding author.School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor 47500, MalaysiaSchool of Biosciences, Taylor's University, Subang Jaya, Selangor 47500, MalaysiaFood Technology Division, School of Industrial Technology, Universiti Sains Malaysia, Minden, Penang 11800, MalaysiaGelatin is a low-cost and widely used soluble protein compound derived from collagen. Black soldier fly (Hermetia illucens) larvae (BSFL) are a potential alternative source of gelatin due to their high nutritional value. This study investigated the effect of acetic acid pretreatment concentration, time, and centrifugation speed on the yield of BSFL gelatin and characterized its physicochemical properties. BSFL gelatin extracted with 0.05 mol/L acetic acid for 3 h and centrifuged at 3000 × g (BSFL3) had the highest yield of 1.085%±0.2%. The BSFL3 gelatin had lower protein content and amino acids than commercial porcine gelatin, specifically proline and hydroxyproline. It also had a high melting temperature of 135.12±1.04 °C, viscosity of 1.69±0.05 mPa·s, and rough microstructure with low foaming properties. The BSFL gelatin is high in K (680/01 mg/kg) and contains less Na, Mg, Ca, and P. Field emission scanning electron microscopy (FE-SEM) and energy dispersive X-ray (EDX) revealed that BSFL3 gelatin has high potassium content. The results suggest that BSFL gelatin can be used as a novel techno-functional ingredient in the food industry.http://www.sciencedirect.com/science/article/pii/S2772753X2300103XBlack soldier fly larvaeGelatinProteinPhysicochemical properties |
| spellingShingle | Lee-Kee Chua Pek-Kui Lim Yin-Yin Thoo Yun-Ping Neo Thuan-Chew Tan Extraction and characterization of gelatin derived from acetic acid-treated black soldier fly larvae Food Chemistry Advances Black soldier fly larvae Gelatin Protein Physicochemical properties |
| title | Extraction and characterization of gelatin derived from acetic acid-treated black soldier fly larvae |
| title_full | Extraction and characterization of gelatin derived from acetic acid-treated black soldier fly larvae |
| title_fullStr | Extraction and characterization of gelatin derived from acetic acid-treated black soldier fly larvae |
| title_full_unstemmed | Extraction and characterization of gelatin derived from acetic acid-treated black soldier fly larvae |
| title_short | Extraction and characterization of gelatin derived from acetic acid-treated black soldier fly larvae |
| title_sort | extraction and characterization of gelatin derived from acetic acid treated black soldier fly larvae |
| topic | Black soldier fly larvae Gelatin Protein Physicochemical properties |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X2300103X |
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