Dietary Supplementation with Epicatechin Improves Intestinal Barrier Integrity in Mice
Epicatechin (EPI) is a dietary flavonoid that is present in many foods and possesses various bioactivities. We assessed the effects of EPI supplementation on intestinal barrier integrity in mice. Thirty-six mice were assigned to three groups and fed a standard diet or a standard diet supplemented wi...
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MDPI AG
2022-10-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/20/3301 |
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| author | Jin Wan Li Zhang Zheng Ruan |
| author_facet | Jin Wan Li Zhang Zheng Ruan |
| author_sort | Jin Wan |
| collection | DOAJ |
| description | Epicatechin (EPI) is a dietary flavonoid that is present in many foods and possesses various bioactivities. We assessed the effects of EPI supplementation on intestinal barrier integrity in mice. Thirty-six mice were assigned to three groups and fed a standard diet or a standard diet supplemented with 50 or 100 mg EPI/kg (<i>n</i> = 12 per group). After 21 days of rearing, blood and intestinal samples were collected from eight randomly selected mice. Supplementation with 50 and 100 mg/kg EPI decreased (<i>p</i> < 0.05) the serum diamine oxidase activity and <sub>D</sub>-lactic acid concentration and increased (<i>p</i> < 0.05) the duodenal, jejunal, and ileal abundance of tight junction proteins, such as occludin. Moreover, it lowered (<i>p</i> < 0.05) the duodenal, jejunal, and ileal tumor necrosis factor-α contents and enhanced (<i>p</i> < 0.05) the duodenal and jejunal catalase activities and ileal superoxide dismutase activity. Supplementation with a lower dose (50 mg/kg) decreased (<i>p</i> < 0.05) the ileal interleukin-1β content, whereas supplementation with a higher dose (100 mg/kg) increased (<i>p</i> < 0.05) the duodenal and jejunal glutathione peroxidase activities. Furthermore, supplementation with 50 and 100 mg/kg EPI decreased (<i>p</i> < 0.05) cell apoptosis, cleaved cysteinyl aspartate-specific proteinase-3 (caspase-3), and cleaved caspase-9 contents in the duodenum, jejunum, and ileum. In conclusion, EPI could improve intestinal barrier integrity in mice, thereby suppressing intestinal inflammation and oxidative stress and reducing cell apoptosis. |
| first_indexed | 2024-03-09T20:13:05Z |
| format | Article |
| id | doaj.art-ec138490ac874bd6b67b4a97ff858c4e |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T20:13:05Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-ec138490ac874bd6b67b4a97ff858c4e2023-11-24T00:08:42ZengMDPI AGFoods2304-81582022-10-011120330110.3390/foods11203301Dietary Supplementation with Epicatechin Improves Intestinal Barrier Integrity in MiceJin Wan0Li Zhang1Zheng Ruan2International Institute of Food Innovation, Nanchang University, Nanchang 330200, ChinaCollege of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaInternational Institute of Food Innovation, Nanchang University, Nanchang 330200, ChinaEpicatechin (EPI) is a dietary flavonoid that is present in many foods and possesses various bioactivities. We assessed the effects of EPI supplementation on intestinal barrier integrity in mice. Thirty-six mice were assigned to three groups and fed a standard diet or a standard diet supplemented with 50 or 100 mg EPI/kg (<i>n</i> = 12 per group). After 21 days of rearing, blood and intestinal samples were collected from eight randomly selected mice. Supplementation with 50 and 100 mg/kg EPI decreased (<i>p</i> < 0.05) the serum diamine oxidase activity and <sub>D</sub>-lactic acid concentration and increased (<i>p</i> < 0.05) the duodenal, jejunal, and ileal abundance of tight junction proteins, such as occludin. Moreover, it lowered (<i>p</i> < 0.05) the duodenal, jejunal, and ileal tumor necrosis factor-α contents and enhanced (<i>p</i> < 0.05) the duodenal and jejunal catalase activities and ileal superoxide dismutase activity. Supplementation with a lower dose (50 mg/kg) decreased (<i>p</i> < 0.05) the ileal interleukin-1β content, whereas supplementation with a higher dose (100 mg/kg) increased (<i>p</i> < 0.05) the duodenal and jejunal glutathione peroxidase activities. Furthermore, supplementation with 50 and 100 mg/kg EPI decreased (<i>p</i> < 0.05) cell apoptosis, cleaved cysteinyl aspartate-specific proteinase-3 (caspase-3), and cleaved caspase-9 contents in the duodenum, jejunum, and ileum. In conclusion, EPI could improve intestinal barrier integrity in mice, thereby suppressing intestinal inflammation and oxidative stress and reducing cell apoptosis.https://www.mdpi.com/2304-8158/11/20/3301epicatechinantioxidant capacityinflammatory responsescell apoptosisintestinal barrier |
| spellingShingle | Jin Wan Li Zhang Zheng Ruan Dietary Supplementation with Epicatechin Improves Intestinal Barrier Integrity in Mice Foods epicatechin antioxidant capacity inflammatory responses cell apoptosis intestinal barrier |
| title | Dietary Supplementation with Epicatechin Improves Intestinal Barrier Integrity in Mice |
| title_full | Dietary Supplementation with Epicatechin Improves Intestinal Barrier Integrity in Mice |
| title_fullStr | Dietary Supplementation with Epicatechin Improves Intestinal Barrier Integrity in Mice |
| title_full_unstemmed | Dietary Supplementation with Epicatechin Improves Intestinal Barrier Integrity in Mice |
| title_short | Dietary Supplementation with Epicatechin Improves Intestinal Barrier Integrity in Mice |
| title_sort | dietary supplementation with epicatechin improves intestinal barrier integrity in mice |
| topic | epicatechin antioxidant capacity inflammatory responses cell apoptosis intestinal barrier |
| url | https://www.mdpi.com/2304-8158/11/20/3301 |
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