Cell Lines for Honey Bee Virus Research
With ongoing colony losses driven in part by the Varroa mite and the associated exacerbation of the virus load, there is an urgent need to protect honey bees (<i>Apis mellifera</i>) from fatal levels of virus infection and from the non-target effects of insecticides used in agricultural...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/12/2/236 |
| _version_ | 1818908905674113024 |
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| author | Ya Guo Cynthia L. Goodman David W. Stanley Bryony C. Bonning |
| author_facet | Ya Guo Cynthia L. Goodman David W. Stanley Bryony C. Bonning |
| author_sort | Ya Guo |
| collection | DOAJ |
| description | With ongoing colony losses driven in part by the Varroa mite and the associated exacerbation of the virus load, there is an urgent need to protect honey bees (<i>Apis mellifera</i>) from fatal levels of virus infection and from the non-target effects of insecticides used in agricultural settings. A continuously replicating cell line derived from the honey bee would provide a valuable tool for the study of molecular mechanisms of virus−host interaction, for the screening of antiviral agents for potential use within the hive, and for the assessment of the risk of current and candidate insecticides to the honey bee. However, the establishment of a continuously replicating honey bee cell line has proved challenging. Here, we provide an overview of attempts to establish primary and continuously replicating hymenopteran cell lines, methods (including recent results) of establishing honey bee cell lines, challenges associated with the presence of latent viruses (especially <i>Deformed wing virus</i>) in established cell lines and methods to establish virus-free cell lines. We also describe the potential use of honey bee cell lines in conjunction with infectious clones of honey bee viruses for examination of fundamental virology. |
| first_indexed | 2024-12-19T22:18:27Z |
| format | Article |
| id | doaj.art-ec1636c8717741b3b347884483bf2a84 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-19T22:18:27Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-ec1636c8717741b3b347884483bf2a842022-12-21T20:03:42ZengMDPI AGViruses1999-49152020-02-0112223610.3390/v12020236v12020236Cell Lines for Honey Bee Virus ResearchYa Guo0Cynthia L. Goodman1David W. Stanley2Bryony C. Bonning3Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611, USABiological Control of Insects Research Laboratory, USDA/Agricultural Research Service, Columbia, MO 65203, USABiological Control of Insects Research Laboratory, USDA/Agricultural Research Service, Columbia, MO 65203, USADepartment of Entomology and Nematology, University of Florida, Gainesville, FL 32611, USAWith ongoing colony losses driven in part by the Varroa mite and the associated exacerbation of the virus load, there is an urgent need to protect honey bees (<i>Apis mellifera</i>) from fatal levels of virus infection and from the non-target effects of insecticides used in agricultural settings. A continuously replicating cell line derived from the honey bee would provide a valuable tool for the study of molecular mechanisms of virus−host interaction, for the screening of antiviral agents for potential use within the hive, and for the assessment of the risk of current and candidate insecticides to the honey bee. However, the establishment of a continuously replicating honey bee cell line has proved challenging. Here, we provide an overview of attempts to establish primary and continuously replicating hymenopteran cell lines, methods (including recent results) of establishing honey bee cell lines, challenges associated with the presence of latent viruses (especially <i>Deformed wing virus</i>) in established cell lines and methods to establish virus-free cell lines. We also describe the potential use of honey bee cell lines in conjunction with infectious clones of honey bee viruses for examination of fundamental virology.https://www.mdpi.com/1999-4915/12/2/236honey bee virushymenopterainsect cell culturecell lines<i>apis mellifera</i>deformed wing virus |
| spellingShingle | Ya Guo Cynthia L. Goodman David W. Stanley Bryony C. Bonning Cell Lines for Honey Bee Virus Research Viruses honey bee virus hymenoptera insect cell culture cell lines <i>apis mellifera</i> deformed wing virus |
| title | Cell Lines for Honey Bee Virus Research |
| title_full | Cell Lines for Honey Bee Virus Research |
| title_fullStr | Cell Lines for Honey Bee Virus Research |
| title_full_unstemmed | Cell Lines for Honey Bee Virus Research |
| title_short | Cell Lines for Honey Bee Virus Research |
| title_sort | cell lines for honey bee virus research |
| topic | honey bee virus hymenoptera insect cell culture cell lines <i>apis mellifera</i> deformed wing virus |
| url | https://www.mdpi.com/1999-4915/12/2/236 |
| work_keys_str_mv | AT yaguo celllinesforhoneybeevirusresearch AT cynthialgoodman celllinesforhoneybeevirusresearch AT davidwstanley celllinesforhoneybeevirusresearch AT bryonycbonning celllinesforhoneybeevirusresearch |