Babesia microti Protein BmSP44 Is a Novel Protective Antigen in a Mouse Model of Babesiosis
Babesiosis caused by Babesia species imposes an increasing threat to public-health and so far, there is no effective vaccine to prevent Babesia infections. Babesia surface antigen may participate in the invasion of erythrocytes. In our previous study, a surface antigen of B. microti merozoites, name...
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| Format: | Article |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2020.01437/full |
| _version_ | 1818406656788135936 |
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| author | Hui Wang Yao Wang Jilei Huang Bin Xu Junhu Chen Jianfeng Dai Xia Zhou |
| author_facet | Hui Wang Yao Wang Jilei Huang Bin Xu Junhu Chen Jianfeng Dai Xia Zhou |
| author_sort | Hui Wang |
| collection | DOAJ |
| description | Babesiosis caused by Babesia species imposes an increasing threat to public-health and so far, there is no effective vaccine to prevent Babesia infections. Babesia surface antigen may participate in the invasion of erythrocytes. In our previous study, a surface antigen of B. microti merozoites, named as BmSP44 was identified as a dominant reactive antigen by protein microarray screening. To evaluate its potential applications in diagnosis and prevention of Babesiosis, the open reading frame encoding BmSP44 was cloned and the recombinant protein was expressed. In consistent with the protein microarray result, recombinant BmSP44 (rBmSP44) can be recognized by sera from B. microti infected mice. Immunofluorescence assays (IFA) confirmed that BmSP44 is a secreted protein and localized principally in the cytoplasm of the parasites. The parasitemia and Babesia gene copies were lower in mice administered rBmSP44 antisera compared with normal controls. Active immunization with rBmSP44 also afforded protection against B. microti infection. The concentrations of hemoglobin in rBmSP44 immunization group were higher than those in the control group. Importantly, vaccination of mice with rBmSP44 resulted in a Th1/Th2 mixed immune response with significantly elevated IL-10 and IFN-γ levels during the early stage of infection. Taken together, our results indicated that rBmSP44 can induce a protective immune response against Babesia infection. Thus, BmSP44 can be used as both a diagnosis marker and a vaccine candidate. |
| first_indexed | 2024-12-14T09:15:25Z |
| format | Article |
| id | doaj.art-ec171ee9c5134263b9dc034878176340 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-14T09:15:25Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-ec171ee9c5134263b9dc0348781763402022-12-21T23:08:28ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-07-011110.3389/fimmu.2020.01437537853Babesia microti Protein BmSP44 Is a Novel Protective Antigen in a Mouse Model of BabesiosisHui Wang0Yao Wang1Jilei Huang2Bin Xu3Junhu Chen4Jianfeng Dai5Xia Zhou6School of Biology and Medical Science, Soochow University Medical College, Suzhou, ChinaSchool of Biology and Medical Science, Soochow University Medical College, Suzhou, ChinaKey Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, National Institute of Parasitic Diseases, Shanghai, ChinaKey Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, National Institute of Parasitic Diseases, Shanghai, ChinaKey Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, National Institute of Parasitic Diseases, Shanghai, ChinaJiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou, ChinaSchool of Biology and Medical Science, Soochow University Medical College, Suzhou, ChinaBabesiosis caused by Babesia species imposes an increasing threat to public-health and so far, there is no effective vaccine to prevent Babesia infections. Babesia surface antigen may participate in the invasion of erythrocytes. In our previous study, a surface antigen of B. microti merozoites, named as BmSP44 was identified as a dominant reactive antigen by protein microarray screening. To evaluate its potential applications in diagnosis and prevention of Babesiosis, the open reading frame encoding BmSP44 was cloned and the recombinant protein was expressed. In consistent with the protein microarray result, recombinant BmSP44 (rBmSP44) can be recognized by sera from B. microti infected mice. Immunofluorescence assays (IFA) confirmed that BmSP44 is a secreted protein and localized principally in the cytoplasm of the parasites. The parasitemia and Babesia gene copies were lower in mice administered rBmSP44 antisera compared with normal controls. Active immunization with rBmSP44 also afforded protection against B. microti infection. The concentrations of hemoglobin in rBmSP44 immunization group were higher than those in the control group. Importantly, vaccination of mice with rBmSP44 resulted in a Th1/Th2 mixed immune response with significantly elevated IL-10 and IFN-γ levels during the early stage of infection. Taken together, our results indicated that rBmSP44 can induce a protective immune response against Babesia infection. Thus, BmSP44 can be used as both a diagnosis marker and a vaccine candidate.https://www.frontiersin.org/article/10.3389/fimmu.2020.01437/fullBabesia microtisecreted proteinvaccineantigensdiagnosis marker |
| spellingShingle | Hui Wang Yao Wang Jilei Huang Bin Xu Junhu Chen Jianfeng Dai Xia Zhou Babesia microti Protein BmSP44 Is a Novel Protective Antigen in a Mouse Model of Babesiosis Frontiers in Immunology Babesia microti secreted protein vaccine antigens diagnosis marker |
| title | Babesia microti Protein BmSP44 Is a Novel Protective Antigen in a Mouse Model of Babesiosis |
| title_full | Babesia microti Protein BmSP44 Is a Novel Protective Antigen in a Mouse Model of Babesiosis |
| title_fullStr | Babesia microti Protein BmSP44 Is a Novel Protective Antigen in a Mouse Model of Babesiosis |
| title_full_unstemmed | Babesia microti Protein BmSP44 Is a Novel Protective Antigen in a Mouse Model of Babesiosis |
| title_short | Babesia microti Protein BmSP44 Is a Novel Protective Antigen in a Mouse Model of Babesiosis |
| title_sort | babesia microti protein bmsp44 is a novel protective antigen in a mouse model of babesiosis |
| topic | Babesia microti secreted protein vaccine antigens diagnosis marker |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2020.01437/full |
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