Morphological, immunohistochemical, and biochemical study on the ameliorative effect of gallic acid against bisphenol A-induced nephrotoxicity in male albino rats
Abstract This study aimed to determine the effect of gallic acid (GA) on ameliorating bisphenol A (BPA) nephrotoxicity in male rat kidneys. Forty rats were assigned randomly into two groups: control (ten animals) and BPA (40 mg/kg bwt) (thirty animals), the second group was divided into three subgro...
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-28860-1 |
| _version_ | 1811171730528403456 |
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| author | Shaimaa M. M. Saleh A. Bakr Mahmoud M. Bassam Al-Salahy Fatma Ahmed Mohamed Moustafa |
| author_facet | Shaimaa M. M. Saleh A. Bakr Mahmoud M. Bassam Al-Salahy Fatma Ahmed Mohamed Moustafa |
| author_sort | Shaimaa M. M. Saleh |
| collection | DOAJ |
| description | Abstract This study aimed to determine the effect of gallic acid (GA) on ameliorating bisphenol A (BPA) nephrotoxicity in male rat kidneys. Forty rats were assigned randomly into two groups: control (ten animals) and BPA (40 mg/kg bwt) (thirty animals), the second group was divided into three subgroups: BPA alone, BPA + G50 (50 mg/kg bwt), and BPA + G200 (200 mg/kg bwt). The biochemical analysis included measurements of the contents of nitric oxide, lipid peroxidation, reactive oxygen species, and cytokines (interleukin-1α and interleukin-6) in the kidney. The antioxidant enzymes catalase and superoxide dismutase were also measured in the kidney. Kidney function was assessed by determining uric acid, urea, and creatinine levels. The morphological investigations included hematoxylin and eosin staining for assessing the general histology and determining the glomerular and corpuscular areas, the tubular cell degeneration mean area, and the mean leukocyte infiltration area. Also, collagen fiber intensity and polysaccharide content were analyzed. Furthermore, immunohistochemical, morphometric, and ultrastructural studies were carried out. The results revealed morphological, immunohistochemical, and biochemical alterations in the kidney. Most of these changes showed a satisfactory improvement of kidney damage when BPA-administered rats were treated with GA at both doses. In conclusion, GA exhibited a strong protective effect against BPA-induced nephrotoxicity. |
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| id | doaj.art-ec1a11e1a7f6430a90740340f38d1a7b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T17:20:00Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-ec1a11e1a7f6430a90740340f38d1a7b2023-02-05T12:11:49ZengNature PortfolioScientific Reports2045-23222023-01-0113112010.1038/s41598-023-28860-1Morphological, immunohistochemical, and biochemical study on the ameliorative effect of gallic acid against bisphenol A-induced nephrotoxicity in male albino ratsShaimaa M. M. Saleh0A. Bakr Mahmoud1M. Bassam Al-Salahy2Fatma Ahmed Mohamed Moustafa3Department of Zoology and Entomology, Faculty of Science, Assiut UniversityDepartment of Zoology and Entomology, Faculty of Science, Assiut UniversityDepartment of Zoology and Entomology, Faculty of Science, Assiut UniversityDepartment of Zoology and Entomology, Faculty of Science, Assiut UniversityAbstract This study aimed to determine the effect of gallic acid (GA) on ameliorating bisphenol A (BPA) nephrotoxicity in male rat kidneys. Forty rats were assigned randomly into two groups: control (ten animals) and BPA (40 mg/kg bwt) (thirty animals), the second group was divided into three subgroups: BPA alone, BPA + G50 (50 mg/kg bwt), and BPA + G200 (200 mg/kg bwt). The biochemical analysis included measurements of the contents of nitric oxide, lipid peroxidation, reactive oxygen species, and cytokines (interleukin-1α and interleukin-6) in the kidney. The antioxidant enzymes catalase and superoxide dismutase were also measured in the kidney. Kidney function was assessed by determining uric acid, urea, and creatinine levels. The morphological investigations included hematoxylin and eosin staining for assessing the general histology and determining the glomerular and corpuscular areas, the tubular cell degeneration mean area, and the mean leukocyte infiltration area. Also, collagen fiber intensity and polysaccharide content were analyzed. Furthermore, immunohistochemical, morphometric, and ultrastructural studies were carried out. The results revealed morphological, immunohistochemical, and biochemical alterations in the kidney. Most of these changes showed a satisfactory improvement of kidney damage when BPA-administered rats were treated with GA at both doses. In conclusion, GA exhibited a strong protective effect against BPA-induced nephrotoxicity.https://doi.org/10.1038/s41598-023-28860-1 |
| spellingShingle | Shaimaa M. M. Saleh A. Bakr Mahmoud M. Bassam Al-Salahy Fatma Ahmed Mohamed Moustafa Morphological, immunohistochemical, and biochemical study on the ameliorative effect of gallic acid against bisphenol A-induced nephrotoxicity in male albino rats Scientific Reports |
| title | Morphological, immunohistochemical, and biochemical study on the ameliorative effect of gallic acid against bisphenol A-induced nephrotoxicity in male albino rats |
| title_full | Morphological, immunohistochemical, and biochemical study on the ameliorative effect of gallic acid against bisphenol A-induced nephrotoxicity in male albino rats |
| title_fullStr | Morphological, immunohistochemical, and biochemical study on the ameliorative effect of gallic acid against bisphenol A-induced nephrotoxicity in male albino rats |
| title_full_unstemmed | Morphological, immunohistochemical, and biochemical study on the ameliorative effect of gallic acid against bisphenol A-induced nephrotoxicity in male albino rats |
| title_short | Morphological, immunohistochemical, and biochemical study on the ameliorative effect of gallic acid against bisphenol A-induced nephrotoxicity in male albino rats |
| title_sort | morphological immunohistochemical and biochemical study on the ameliorative effect of gallic acid against bisphenol a induced nephrotoxicity in male albino rats |
| url | https://doi.org/10.1038/s41598-023-28860-1 |
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