COMPARATIVE PATHOLOGICAL STUDY OF EXPERIMENTAL INFECTION OF NEWCASTLE DISEASE VIRUS GENOTYPES VII IN CHICKENS AND DUCKS
Newcastle disease virus (NDV) is a highly contagious virus infects a wide range of avian species including chicken and ducks. The disease can be fatal for many types of them. It was believed that ducks and geese act as natural reservoirs of NDV because of their natural resistance. Recently, NDV iso...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2021-12-01
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| Series: | Slovenian Veterinary Research |
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| Online Access: | https://www.slovetres.si/index.php/SVR/article/view/1444 |
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| author | Yassmin EL-Morshidy Nagwan El-Habashi Ghada Abd EL–Dayem Ahmed El sawak Walied Abdo |
| author_facet | Yassmin EL-Morshidy Nagwan El-Habashi Ghada Abd EL–Dayem Ahmed El sawak Walied Abdo |
| author_sort | Yassmin EL-Morshidy |
| collection | DOAJ |
| description |
Newcastle disease virus (NDV) is a highly contagious virus infects a wide range of avian species including chicken and ducks. The disease can be fatal for many types of them. It was believed that ducks and geese act as natural reservoirs of NDV because of their natural resistance. Recently, NDV isolates were pathogenic for ducks. Therefore, our study aimed to investigate the pathogenicity of highly pathogenic NDV genotype VII in white Ross commercial broiler chickens and Mallard ducks on the basis of clinical, histological observation and immunohistochemistry findings of virus antigen through studying the sequential pathological lesions of both species at 2nd, 5th and 10th dpi. Both species were inoculated via intranasal route by NDV genotype VII (accession number MT887290). Clinical lesions and mortalities were evident mostly in chickens rather than in ducks. Respiratory, digestive and nervous signs were clearly manifested in chickens. Ducks showed low level of mortalities and the nervous signs were the most obvious symptoms. Microscopic findings of chickens showed severe pancreatitis, pneumonia, enteritis as well as pericarditis and brain malacia. Similar findings were noticed in ducks but with lower degree of severity. Brain lesions in ducks included encephalitis and perivascular reaction mostly within the cerebellar tissue. Immunohistochemistry reaction against NDV antigen showed marked immunostaining within epithelial cells, purkinje cells and inflammatory cells mostly the macrophages. The immunohistochemistry reaction was correlated with the pathological findings in both species. In conclusion, the mortality and lesions of NDV in both species were correlated with systemic alterations associated with pancreatitis, pneumonia and encephalopathy. Moreover, the obtained data revealed possible susceptibility of ducks to NDV genotype VII, which might be need forthcoming viral vaccination strategy for ducks against NDV.
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| first_indexed | 2024-04-24T14:02:45Z |
| format | Article |
| id | doaj.art-ec1f21686b0f4ca6a42fb74471830350 |
| institution | Directory Open Access Journal |
| issn | 1580-4003 2385-8761 |
| language | English |
| last_indexed | 2024-04-24T14:02:45Z |
| publishDate | 2021-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Slovenian Veterinary Research |
| spelling | doaj.art-ec1f21686b0f4ca6a42fb744718303502024-04-03T12:38:25ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Slovenian Veterinary Research1580-40032385-87612021-12-015824-Suppl10.26873/SVR-1444-2021COMPARATIVE PATHOLOGICAL STUDY OF EXPERIMENTAL INFECTION OF NEWCASTLE DISEASE VIRUS GENOTYPES VII IN CHICKENS AND DUCKSYassmin EL-Morshidy0Nagwan El-Habashi1Ghada Abd EL–Dayem2Ahmed El sawak3Walied Abdo4Department of Veterinary Pathology, Animal Health Research Institute(AHRI), Agriculture Research Center (ARC), Dokki, Giza, EgyptDepartment of Veterinary Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516- Kafrelsheikh, EgyptDepartment of poultry diseases, Animal Health Research Institute(AHRI), Agriculture Research Center (ARC), P.O. Box 246 Dokki, 12618 – Giza, EgyptDepartment of Veterinary Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516- Kafrelsheikh, EgyptDepartment of Veterinary Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516- Kafrelsheikh, Egypt Newcastle disease virus (NDV) is a highly contagious virus infects a wide range of avian species including chicken and ducks. The disease can be fatal for many types of them. It was believed that ducks and geese act as natural reservoirs of NDV because of their natural resistance. Recently, NDV isolates were pathogenic for ducks. Therefore, our study aimed to investigate the pathogenicity of highly pathogenic NDV genotype VII in white Ross commercial broiler chickens and Mallard ducks on the basis of clinical, histological observation and immunohistochemistry findings of virus antigen through studying the sequential pathological lesions of both species at 2nd, 5th and 10th dpi. Both species were inoculated via intranasal route by NDV genotype VII (accession number MT887290). Clinical lesions and mortalities were evident mostly in chickens rather than in ducks. Respiratory, digestive and nervous signs were clearly manifested in chickens. Ducks showed low level of mortalities and the nervous signs were the most obvious symptoms. Microscopic findings of chickens showed severe pancreatitis, pneumonia, enteritis as well as pericarditis and brain malacia. Similar findings were noticed in ducks but with lower degree of severity. Brain lesions in ducks included encephalitis and perivascular reaction mostly within the cerebellar tissue. Immunohistochemistry reaction against NDV antigen showed marked immunostaining within epithelial cells, purkinje cells and inflammatory cells mostly the macrophages. The immunohistochemistry reaction was correlated with the pathological findings in both species. In conclusion, the mortality and lesions of NDV in both species were correlated with systemic alterations associated with pancreatitis, pneumonia and encephalopathy. Moreover, the obtained data revealed possible susceptibility of ducks to NDV genotype VII, which might be need forthcoming viral vaccination strategy for ducks against NDV. https://www.slovetres.si/index.php/SVR/article/view/1444Newcastle Diseasechickenduckvirulent NDV-genotype VII challenge |
| spellingShingle | Yassmin EL-Morshidy Nagwan El-Habashi Ghada Abd EL–Dayem Ahmed El sawak Walied Abdo COMPARATIVE PATHOLOGICAL STUDY OF EXPERIMENTAL INFECTION OF NEWCASTLE DISEASE VIRUS GENOTYPES VII IN CHICKENS AND DUCKS Slovenian Veterinary Research Newcastle Disease chicken duck virulent NDV-genotype VII challenge |
| title | COMPARATIVE PATHOLOGICAL STUDY OF EXPERIMENTAL INFECTION OF NEWCASTLE DISEASE VIRUS GENOTYPES VII IN CHICKENS AND DUCKS |
| title_full | COMPARATIVE PATHOLOGICAL STUDY OF EXPERIMENTAL INFECTION OF NEWCASTLE DISEASE VIRUS GENOTYPES VII IN CHICKENS AND DUCKS |
| title_fullStr | COMPARATIVE PATHOLOGICAL STUDY OF EXPERIMENTAL INFECTION OF NEWCASTLE DISEASE VIRUS GENOTYPES VII IN CHICKENS AND DUCKS |
| title_full_unstemmed | COMPARATIVE PATHOLOGICAL STUDY OF EXPERIMENTAL INFECTION OF NEWCASTLE DISEASE VIRUS GENOTYPES VII IN CHICKENS AND DUCKS |
| title_short | COMPARATIVE PATHOLOGICAL STUDY OF EXPERIMENTAL INFECTION OF NEWCASTLE DISEASE VIRUS GENOTYPES VII IN CHICKENS AND DUCKS |
| title_sort | comparative pathological study of experimental infection of newcastle disease virus genotypes vii in chickens and ducks |
| topic | Newcastle Disease chicken duck virulent NDV-genotype VII challenge |
| url | https://www.slovetres.si/index.php/SVR/article/view/1444 |
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