Genes Modulating the Increase in Sexuality in the Facultative Diplosporous Grass <i>Eragrostis curvula</i> under Water Stress Conditions
<i>Eragrostis curvula</i> presents mainly facultative genotypes that reproduce by diplosporous apomixis, retaining a percentage of sexual pistils that increase under drought and other stressful situations, indicating that some regulators activated by stress could be affecting the apomixi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/9/969 |
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| author | Juan Pablo Selva Diego Zappacosta José Carballo Juan Manuel Rodrigo Andrés Bellido Cristian Andrés Gallo Jimena Gallardo Viviana Echenique |
| author_facet | Juan Pablo Selva Diego Zappacosta José Carballo Juan Manuel Rodrigo Andrés Bellido Cristian Andrés Gallo Jimena Gallardo Viviana Echenique |
| author_sort | Juan Pablo Selva |
| collection | DOAJ |
| description | <i>Eragrostis curvula</i> presents mainly facultative genotypes that reproduce by diplosporous apomixis, retaining a percentage of sexual pistils that increase under drought and other stressful situations, indicating that some regulators activated by stress could be affecting the apomixis/sexual switch. Water stress experiments were performed in order to associate the increase in sexual embryo sacs with the differential expression of genes in a facultative apomictic cultivar using cytoembryology and RNA sequencing. The percentage of sexual embryo sacs increased from 4 to 24% and 501 out of the 201,011 transcripts were differentially expressed (DE) between control and stressed plants. DE transcripts were compared with previous transcriptomes where apomictic and sexual genotypes were contrasted. The results point as candidates to transcripts related to methylation, ubiquitination, hormone and signal transduction pathways, transcription regulation and cell wall biosynthesis, some acting as a general response to stress and some that are specific to the reproductive mode. We suggest that a DNA glycosylase EcROS1-like could be demethylating, thus de-repressing a gene or genes involved in the sexuality pathways. Many of the other DE transcripts could be part of a complex mechanism that regulates apomixis and sexuality in this grass, the ones in the intersection between control/stress and apo/sex being the strongest candidates. |
| first_indexed | 2024-03-10T17:04:24Z |
| format | Article |
| id | doaj.art-ec1fe1791adf45c2a3037127b0397a03 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T17:04:24Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-ec1fe1791adf45c2a3037127b0397a032023-11-20T10:52:44ZengMDPI AGGenes2073-44252020-08-0111996910.3390/genes11090969Genes Modulating the Increase in Sexuality in the Facultative Diplosporous Grass <i>Eragrostis curvula</i> under Water Stress ConditionsJuan Pablo Selva0Diego Zappacosta1José Carballo2Juan Manuel Rodrigo3Andrés Bellido4Cristian Andrés Gallo5Jimena Gallardo6Viviana Echenique7Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS—CCT—CONICET Bahía Blanca), Camino de la Carrindanga km 7, Bahía Blanca 8000, ArgentinaCentro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS—CCT—CONICET Bahía Blanca), Camino de la Carrindanga km 7, Bahía Blanca 8000, ArgentinaCentro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS—CCT—CONICET Bahía Blanca), Camino de la Carrindanga km 7, Bahía Blanca 8000, ArgentinaCentro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS—CCT—CONICET Bahía Blanca), Camino de la Carrindanga km 7, Bahía Blanca 8000, ArgentinaCentro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS—CCT—CONICET Bahía Blanca), Camino de la Carrindanga km 7, Bahía Blanca 8000, ArgentinaCentro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS—CCT—CONICET Bahía Blanca), Camino de la Carrindanga km 7, Bahía Blanca 8000, ArgentinaCentro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS—CCT—CONICET Bahía Blanca), Camino de la Carrindanga km 7, Bahía Blanca 8000, ArgentinaCentro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS—CCT—CONICET Bahía Blanca), Camino de la Carrindanga km 7, Bahía Blanca 8000, Argentina<i>Eragrostis curvula</i> presents mainly facultative genotypes that reproduce by diplosporous apomixis, retaining a percentage of sexual pistils that increase under drought and other stressful situations, indicating that some regulators activated by stress could be affecting the apomixis/sexual switch. Water stress experiments were performed in order to associate the increase in sexual embryo sacs with the differential expression of genes in a facultative apomictic cultivar using cytoembryology and RNA sequencing. The percentage of sexual embryo sacs increased from 4 to 24% and 501 out of the 201,011 transcripts were differentially expressed (DE) between control and stressed plants. DE transcripts were compared with previous transcriptomes where apomictic and sexual genotypes were contrasted. The results point as candidates to transcripts related to methylation, ubiquitination, hormone and signal transduction pathways, transcription regulation and cell wall biosynthesis, some acting as a general response to stress and some that are specific to the reproductive mode. We suggest that a DNA glycosylase EcROS1-like could be demethylating, thus de-repressing a gene or genes involved in the sexuality pathways. Many of the other DE transcripts could be part of a complex mechanism that regulates apomixis and sexuality in this grass, the ones in the intersection between control/stress and apo/sex being the strongest candidates.https://www.mdpi.com/2073-4425/11/9/969weeping lovegrassapomixisdrought stressRNA-seqdifferentially expressed genessexuality |
| spellingShingle | Juan Pablo Selva Diego Zappacosta José Carballo Juan Manuel Rodrigo Andrés Bellido Cristian Andrés Gallo Jimena Gallardo Viviana Echenique Genes Modulating the Increase in Sexuality in the Facultative Diplosporous Grass <i>Eragrostis curvula</i> under Water Stress Conditions Genes weeping lovegrass apomixis drought stress RNA-seq differentially expressed genes sexuality |
| title | Genes Modulating the Increase in Sexuality in the Facultative Diplosporous Grass <i>Eragrostis curvula</i> under Water Stress Conditions |
| title_full | Genes Modulating the Increase in Sexuality in the Facultative Diplosporous Grass <i>Eragrostis curvula</i> under Water Stress Conditions |
| title_fullStr | Genes Modulating the Increase in Sexuality in the Facultative Diplosporous Grass <i>Eragrostis curvula</i> under Water Stress Conditions |
| title_full_unstemmed | Genes Modulating the Increase in Sexuality in the Facultative Diplosporous Grass <i>Eragrostis curvula</i> under Water Stress Conditions |
| title_short | Genes Modulating the Increase in Sexuality in the Facultative Diplosporous Grass <i>Eragrostis curvula</i> under Water Stress Conditions |
| title_sort | genes modulating the increase in sexuality in the facultative diplosporous grass i eragrostis curvula i under water stress conditions |
| topic | weeping lovegrass apomixis drought stress RNA-seq differentially expressed genes sexuality |
| url | https://www.mdpi.com/2073-4425/11/9/969 |
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