Biochemical and insecticidal efficacy of clove and basil essential oils and two photosensitizers and their combinations on Aphis gossypii glover (Hemiptera: Aphididae)
The present study investigates the insecticidal and biochemical effects of two essential oils (EOs) and two photosensitizers against cotton aphids in a laboratory setting. The EOs evaluated were clove (Syzygium aromaticum L.) and basil (Ocimum basilicum), while the photosensitizers were rose bengal...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Uberlândia
2023-10-01
|
| Series: | Bioscience Journal |
| Subjects: | |
| Online Access: | https://seer.ufu.br/index.php/biosciencejournal/article/view/69129 |
| _version_ | 1797321601042087936 |
|---|---|
| author | Nancy M. El-Shourbagy Shaimaa M. Farag Moataz A. M. Moustafa Laila Al-Shuraym Samy Sayed Ola Zyaan |
| author_facet | Nancy M. El-Shourbagy Shaimaa M. Farag Moataz A. M. Moustafa Laila Al-Shuraym Samy Sayed Ola Zyaan |
| author_sort | Nancy M. El-Shourbagy |
| collection | DOAJ |
| description | The present study investigates the insecticidal and biochemical effects of two essential oils (EOs) and two photosensitizers against cotton aphids in a laboratory setting. The EOs evaluated were clove (Syzygium aromaticum L.) and basil (Ocimum basilicum), while the photosensitizers were rose bengal and rhodamine B. The individual median lethal concentrations (LC50) revealed that clove was ~4.44 times more potent than basil, and rhodamine B was ~1.34 times more potent than rose bengal. The mortality rates increased using higher concentrations of the photosensitizers and prolonging exposure time to sunlight. The most effective combination against adult aphids was found to be a mixture of sub-lethal concentrations of clove and rhodamine B, resulting in a mortality rate of 92.31%. Conversely, the combination of basil and rose bengal exhibited the lowest efficacy with a mortality rate of 33.33%. Biochemical analyses indicate that Rhodamine B, basil, and the basil-rhodamine B mixture (mixture C) significantly reduced trehalase activity. However, the protease activity significantly increased in aphids treated with rose bengal, clove, and the clove-rose bengal mixtures (mixtures A and B). The lipase activity is notably decreased upon treatment with rhodamine B and clove. Glutathione S-transferase (GST) activity decreased in aphids treated with rose bengal and the basil-rhodamine B mixtures (mixtures C and D), suggesting that GST did not play a role in detoxifying these compounds, thereby explaining the susceptibility of A. gossypii. Overall, the combination of essential oils and photosensitizers has demonstrated a synergistic effect in controlling Aphis gossypii, offering great potential as an effective strategy for aphid management. |
| first_indexed | 2024-03-08T05:00:57Z |
| format | Article |
| id | doaj.art-ec214413cef34cd59d69152c76036486 |
| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-03-08T05:00:57Z |
| publishDate | 2023-10-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-ec214413cef34cd59d69152c760364862024-02-07T12:47:43ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632023-10-0139e39100e3910010.14393/BJ-v39n0a2023-6912970828Biochemical and insecticidal efficacy of clove and basil essential oils and two photosensitizers and their combinations on Aphis gossypii glover (Hemiptera: Aphididae)Nancy M. El-Shourbagy0Shaimaa M. Farag1Moataz A. M. Moustafa2Laila Al-Shuraym3https://orcid.org/0000-0002-1378-9579Samy Sayed4https://orcid.org/0000-0002-7002-568XOla Zyaan5https://orcid.org/0000-0001-8200-3261Benha UniversityAin Shams UniversityCairo UniversityPrincess Nourah Bint Abdulrahman UniversityCairo UniversityAin Shams University The present study investigates the insecticidal and biochemical effects of two essential oils (EOs) and two photosensitizers against cotton aphids in a laboratory setting. The EOs evaluated were clove (Syzygium aromaticum L.) and basil (Ocimum basilicum), while the photosensitizers were rose bengal and rhodamine B. The individual median lethal concentrations (LC50) revealed that clove was ~4.44 times more potent than basil, and rhodamine B was ~1.34 times more potent than rose bengal. The mortality rates increased using higher concentrations of the photosensitizers and prolonging exposure time to sunlight. The most effective combination against adult aphids was found to be a mixture of sub-lethal concentrations of clove and rhodamine B, resulting in a mortality rate of 92.31%. Conversely, the combination of basil and rose bengal exhibited the lowest efficacy with a mortality rate of 33.33%. Biochemical analyses indicate that Rhodamine B, basil, and the basil-rhodamine B mixture (mixture C) significantly reduced trehalase activity. However, the protease activity significantly increased in aphids treated with rose bengal, clove, and the clove-rose bengal mixtures (mixtures A and B). The lipase activity is notably decreased upon treatment with rhodamine B and clove. Glutathione S-transferase (GST) activity decreased in aphids treated with rose bengal and the basil-rhodamine B mixtures (mixtures C and D), suggesting that GST did not play a role in detoxifying these compounds, thereby explaining the susceptibility of A. gossypii. Overall, the combination of essential oils and photosensitizers has demonstrated a synergistic effect in controlling Aphis gossypii, offering great potential as an effective strategy for aphid management.https://seer.ufu.br/index.php/biosciencejournal/article/view/69129aphis gossypiibasilclovejoint actionrhodamine b. rose bengal. |
| spellingShingle | Nancy M. El-Shourbagy Shaimaa M. Farag Moataz A. M. Moustafa Laila Al-Shuraym Samy Sayed Ola Zyaan Biochemical and insecticidal efficacy of clove and basil essential oils and two photosensitizers and their combinations on Aphis gossypii glover (Hemiptera: Aphididae) Bioscience Journal aphis gossypii basil clove joint action rhodamine b. rose bengal. |
| title | Biochemical and insecticidal efficacy of clove and basil essential oils and two photosensitizers and their combinations on Aphis gossypii glover (Hemiptera: Aphididae) |
| title_full | Biochemical and insecticidal efficacy of clove and basil essential oils and two photosensitizers and their combinations on Aphis gossypii glover (Hemiptera: Aphididae) |
| title_fullStr | Biochemical and insecticidal efficacy of clove and basil essential oils and two photosensitizers and their combinations on Aphis gossypii glover (Hemiptera: Aphididae) |
| title_full_unstemmed | Biochemical and insecticidal efficacy of clove and basil essential oils and two photosensitizers and their combinations on Aphis gossypii glover (Hemiptera: Aphididae) |
| title_short | Biochemical and insecticidal efficacy of clove and basil essential oils and two photosensitizers and their combinations on Aphis gossypii glover (Hemiptera: Aphididae) |
| title_sort | biochemical and insecticidal efficacy of clove and basil essential oils and two photosensitizers and their combinations on aphis gossypii glover hemiptera aphididae |
| topic | aphis gossypii basil clove joint action rhodamine b. rose bengal. |
| url | https://seer.ufu.br/index.php/biosciencejournal/article/view/69129 |
| work_keys_str_mv | AT nancymelshourbagy biochemicalandinsecticidalefficacyofcloveandbasilessentialoilsandtwophotosensitizersandtheircombinationsonaphisgossypiigloverhemipteraaphididae AT shaimaamfarag biochemicalandinsecticidalefficacyofcloveandbasilessentialoilsandtwophotosensitizersandtheircombinationsonaphisgossypiigloverhemipteraaphididae AT moatazammoustafa biochemicalandinsecticidalefficacyofcloveandbasilessentialoilsandtwophotosensitizersandtheircombinationsonaphisgossypiigloverhemipteraaphididae AT lailaalshuraym biochemicalandinsecticidalefficacyofcloveandbasilessentialoilsandtwophotosensitizersandtheircombinationsonaphisgossypiigloverhemipteraaphididae AT samysayed biochemicalandinsecticidalefficacyofcloveandbasilessentialoilsandtwophotosensitizersandtheircombinationsonaphisgossypiigloverhemipteraaphididae AT olazyaan biochemicalandinsecticidalefficacyofcloveandbasilessentialoilsandtwophotosensitizersandtheircombinationsonaphisgossypiigloverhemipteraaphididae |