Pengembangan Antibodi Poliklonal dari Stadium Oosista, Sporosista, dan Sporozoit Eimeria tenella (THE DEVELOPMENT OF POLYCLONAL ANTOBODY FROM EIMERIA TENELLA OOCYST, SPOROCYST, AND SPOROZOITE STADIUM)
The research on developing diagnostic method, vaccine, and drugs for coccidiosis has been focused onthe finding of the immunogenic molecule in Eimeria. The identification of this agent will need the antibodywhich can recognize the biomolecule in the antigen. Antibody that has been developed for this...
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| Format: | Article |
| Language: | English |
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Universitas Udayana
2013-09-01
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| Series: | Jurnal Veteriner |
| Subjects: | |
| Online Access: | http://ojs.unud.ac.id/index.php/jvet/article/view/6439 |
| _version_ | 1818921096334802944 |
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| author | Galuh Tresnani Joko Prastowo Wisnu Nurcahyo Budi Setiadi Daryono |
| author_facet | Galuh Tresnani Joko Prastowo Wisnu Nurcahyo Budi Setiadi Daryono |
| author_sort | Galuh Tresnani |
| collection | DOAJ |
| description | The research on developing diagnostic method, vaccine, and drugs for coccidiosis has been focused onthe finding of the immunogenic molecule in Eimeria. The identification of this agent will need the antibodywhich can recognize the biomolecule in the antigen. Antibody that has been developed for this purposeshould be analyzed first, and one of the simple methods for analyzing this antibody is through dot blotanalysis. The objective of this research was to analyze the polyclonal antibody which developed from theoocyst, sporocyst, and sporozoite of E. tenella using dot blot analysis. The antigen for this polyclonalantibody was made from each of the E. tenella stadium by sonication. Fifteen mice, divided into 3 groups,were then injected subcutaneously with each antigen. The sera from these mice were then collected, analyzedby using ELISA and then it will be used for the dot blot analysis. The research result showed that thepolyclonal antibody which has been developed in mice from each antigen can react with the antigen itself.From this result it can be concluded that the developing of this antibody is successful and it can be used forfurther research in immunoproteomic. |
| first_indexed | 2024-12-20T01:32:13Z |
| format | Article |
| id | doaj.art-ec2275866f47425faab5f5cb8cd340bf |
| institution | Directory Open Access Journal |
| issn | 1411-8327 2477-5665 |
| language | English |
| last_indexed | 2024-12-20T01:32:13Z |
| publishDate | 2013-09-01 |
| publisher | Universitas Udayana |
| record_format | Article |
| series | Jurnal Veteriner |
| spelling | doaj.art-ec2275866f47425faab5f5cb8cd340bf2022-12-21T19:58:06ZengUniversitas UdayanaJurnal Veteriner1411-83272477-56652013-09-011425843Pengembangan Antibodi Poliklonal dari Stadium Oosista, Sporosista, dan Sporozoit Eimeria tenella (THE DEVELOPMENT OF POLYCLONAL ANTOBODY FROM EIMERIA TENELLA OOCYST, SPOROCYST, AND SPOROZOITE STADIUM)Galuh Tresnani0Joko PrastowoWisnu NurcahyoBudi Setiadi DaryonoBagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, BaliThe research on developing diagnostic method, vaccine, and drugs for coccidiosis has been focused onthe finding of the immunogenic molecule in Eimeria. The identification of this agent will need the antibodywhich can recognize the biomolecule in the antigen. Antibody that has been developed for this purposeshould be analyzed first, and one of the simple methods for analyzing this antibody is through dot blotanalysis. The objective of this research was to analyze the polyclonal antibody which developed from theoocyst, sporocyst, and sporozoite of E. tenella using dot blot analysis. The antigen for this polyclonalantibody was made from each of the E. tenella stadium by sonication. Fifteen mice, divided into 3 groups,were then injected subcutaneously with each antigen. The sera from these mice were then collected, analyzedby using ELISA and then it will be used for the dot blot analysis. The research result showed that thepolyclonal antibody which has been developed in mice from each antigen can react with the antigen itself.From this result it can be concluded that the developing of this antibody is successful and it can be used forfurther research in immunoproteomic.http://ojs.unud.ac.id/index.php/jvet/article/view/6439analisa dot blot, antibodi poliklonal, E. Tenella |
| spellingShingle | Galuh Tresnani Joko Prastowo Wisnu Nurcahyo Budi Setiadi Daryono Pengembangan Antibodi Poliklonal dari Stadium Oosista, Sporosista, dan Sporozoit Eimeria tenella (THE DEVELOPMENT OF POLYCLONAL ANTOBODY FROM EIMERIA TENELLA OOCYST, SPOROCYST, AND SPOROZOITE STADIUM) Jurnal Veteriner analisa dot blot, antibodi poliklonal, E. Tenella |
| title | Pengembangan Antibodi Poliklonal dari Stadium Oosista, Sporosista, dan Sporozoit Eimeria tenella (THE DEVELOPMENT OF POLYCLONAL ANTOBODY FROM EIMERIA TENELLA OOCYST, SPOROCYST, AND SPOROZOITE STADIUM) |
| title_full | Pengembangan Antibodi Poliklonal dari Stadium Oosista, Sporosista, dan Sporozoit Eimeria tenella (THE DEVELOPMENT OF POLYCLONAL ANTOBODY FROM EIMERIA TENELLA OOCYST, SPOROCYST, AND SPOROZOITE STADIUM) |
| title_fullStr | Pengembangan Antibodi Poliklonal dari Stadium Oosista, Sporosista, dan Sporozoit Eimeria tenella (THE DEVELOPMENT OF POLYCLONAL ANTOBODY FROM EIMERIA TENELLA OOCYST, SPOROCYST, AND SPOROZOITE STADIUM) |
| title_full_unstemmed | Pengembangan Antibodi Poliklonal dari Stadium Oosista, Sporosista, dan Sporozoit Eimeria tenella (THE DEVELOPMENT OF POLYCLONAL ANTOBODY FROM EIMERIA TENELLA OOCYST, SPOROCYST, AND SPOROZOITE STADIUM) |
| title_short | Pengembangan Antibodi Poliklonal dari Stadium Oosista, Sporosista, dan Sporozoit Eimeria tenella (THE DEVELOPMENT OF POLYCLONAL ANTOBODY FROM EIMERIA TENELLA OOCYST, SPOROCYST, AND SPOROZOITE STADIUM) |
| title_sort | pengembangan antibodi poliklonal dari stadium oosista sporosista dan sporozoit eimeria tenella the development of polyclonal antobody from eimeria tenella oocyst sporocyst and sporozoite stadium |
| topic | analisa dot blot, antibodi poliklonal, E. Tenella |
| url | http://ojs.unud.ac.id/index.php/jvet/article/view/6439 |
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