Evolution and Expression of the Membrane Attack Complex and Perforin Gene Family in the Poaceae
Membrane Attack Complex and Perforin (MACPF) proteins play crucial roles in plant development and plant responses to environmental stresses. To date, only four <i>MACPF</i> genes have been identified in <i>Arabidopsis thaliana</i>, and the functions of the <i>MACPF</...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/16/5736 |
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| author | Lujun Yu Di Liu Shiyi Chen Yangshuo Dai Wuxiu Guo Xue Zhang Linna Wang Sirui Ma Ming Xiao Hua Qi Shi Xiao Qinfang Chen |
| author_facet | Lujun Yu Di Liu Shiyi Chen Yangshuo Dai Wuxiu Guo Xue Zhang Linna Wang Sirui Ma Ming Xiao Hua Qi Shi Xiao Qinfang Chen |
| author_sort | Lujun Yu |
| collection | DOAJ |
| description | Membrane Attack Complex and Perforin (MACPF) proteins play crucial roles in plant development and plant responses to environmental stresses. To date, only four <i>MACPF</i> genes have been identified in <i>Arabidopsis thaliana</i>, and the functions of the <i>MACPF</i> gene family members in other plants, especially in important crop plants, such as the Poaceae family, remain largely unknown. In this study, we identified and analyzed 42 <i>MACPF</i> genes from six completely sequenced and well annotated species representing the major Poaceae clades. A phylogenetic analysis of <i>MACPF</i> genes resolved four groups, characterized by shared motif organizations and gene structures within each group. <i>MACPF</i> genes were unevenly distributed along the Poaceae chromosomes. Moreover, segmental duplications and dispersed duplication events may have played significant roles during <i>MACPF</i> gene family expansion and functional diversification in the Poaceae. In addition, phylogenomic synteny analysis revealed a high degree of conservation among the Poaceae <i>MACPF</i> genes. In particular, Group I, II, and III <i>MACPF</i> genes were exposed to strong purifying selection with different evolutionary rates. Temporal and spatial expression analyses suggested that Group III <i>MACPF</i> genes were highly expressed relative to the other groups. In addition, most <i>MACPF</i> genes were highly expressed in vegetative tissues and up-regulated by several biotic and abiotic stresses. Taken together, these findings provide valuable information for further functional characterization and phenotypic validation of the Poaceae <i>MACPF</i> gene family. |
| first_indexed | 2024-03-10T17:41:17Z |
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| id | doaj.art-ec23e227562c4e879e8e249f8d9cb389 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T17:41:17Z |
| publishDate | 2020-08-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ec23e227562c4e879e8e249f8d9cb3892023-11-20T09:42:15ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012116573610.3390/ijms21165736Evolution and Expression of the Membrane Attack Complex and Perforin Gene Family in the PoaceaeLujun Yu0Di Liu1Shiyi Chen2Yangshuo Dai3Wuxiu Guo4Xue Zhang5Linna Wang6Sirui Ma7Ming Xiao8Hua Qi9Shi Xiao10Qinfang Chen11State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaMembrane Attack Complex and Perforin (MACPF) proteins play crucial roles in plant development and plant responses to environmental stresses. To date, only four <i>MACPF</i> genes have been identified in <i>Arabidopsis thaliana</i>, and the functions of the <i>MACPF</i> gene family members in other plants, especially in important crop plants, such as the Poaceae family, remain largely unknown. In this study, we identified and analyzed 42 <i>MACPF</i> genes from six completely sequenced and well annotated species representing the major Poaceae clades. A phylogenetic analysis of <i>MACPF</i> genes resolved four groups, characterized by shared motif organizations and gene structures within each group. <i>MACPF</i> genes were unevenly distributed along the Poaceae chromosomes. Moreover, segmental duplications and dispersed duplication events may have played significant roles during <i>MACPF</i> gene family expansion and functional diversification in the Poaceae. In addition, phylogenomic synteny analysis revealed a high degree of conservation among the Poaceae <i>MACPF</i> genes. In particular, Group I, II, and III <i>MACPF</i> genes were exposed to strong purifying selection with different evolutionary rates. Temporal and spatial expression analyses suggested that Group III <i>MACPF</i> genes were highly expressed relative to the other groups. In addition, most <i>MACPF</i> genes were highly expressed in vegetative tissues and up-regulated by several biotic and abiotic stresses. Taken together, these findings provide valuable information for further functional characterization and phenotypic validation of the Poaceae <i>MACPF</i> gene family.https://www.mdpi.com/1422-0067/21/16/5736MACPFevolutionexpression profilePoaceae |
| spellingShingle | Lujun Yu Di Liu Shiyi Chen Yangshuo Dai Wuxiu Guo Xue Zhang Linna Wang Sirui Ma Ming Xiao Hua Qi Shi Xiao Qinfang Chen Evolution and Expression of the Membrane Attack Complex and Perforin Gene Family in the Poaceae International Journal of Molecular Sciences MACPF evolution expression profile Poaceae |
| title | Evolution and Expression of the Membrane Attack Complex and Perforin Gene Family in the Poaceae |
| title_full | Evolution and Expression of the Membrane Attack Complex and Perforin Gene Family in the Poaceae |
| title_fullStr | Evolution and Expression of the Membrane Attack Complex and Perforin Gene Family in the Poaceae |
| title_full_unstemmed | Evolution and Expression of the Membrane Attack Complex and Perforin Gene Family in the Poaceae |
| title_short | Evolution and Expression of the Membrane Attack Complex and Perforin Gene Family in the Poaceae |
| title_sort | evolution and expression of the membrane attack complex and perforin gene family in the poaceae |
| topic | MACPF evolution expression profile Poaceae |
| url | https://www.mdpi.com/1422-0067/21/16/5736 |
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