Facile Detection of Vitamin B12 with Copper Oxide Nanocrystal Graphenic Composite Electrode
Vitamin B12 (VB12) is applied as the cofactors in various important enzymatic reactions and is involved in gene expression regulation mediated by B12-riboswitch and the VB12-dependent photoreceptor. Rapid detection VB12 concertation in a given environment may provide insights in the evaluation of mi...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-06-01
|
| Series: | Water |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4441/13/13/1790 |
| _version_ | 1797411529262366720 |
|---|---|
| author | Chao Tian Na Zhao Xinyue Jiang Duanji Wan Yuqun Xie |
| author_facet | Chao Tian Na Zhao Xinyue Jiang Duanji Wan Yuqun Xie |
| author_sort | Chao Tian |
| collection | DOAJ |
| description | Vitamin B12 (VB12) is applied as the cofactors in various important enzymatic reactions and is involved in gene expression regulation mediated by B12-riboswitch and the VB12-dependent photoreceptor. Rapid detection VB12 concertation in a given environment may provide insights in the evaluation of micronutrient levels and the physiological and ecological performances of organisms under the relevant condition. This study demonstrating an amperometric approach to quantify the VB12 in biological samples without complicated sample pretreatment. The electrochemical oxidation step was conducted with a plain graphenic electrode to convert all nitrogen groups within the VB12 molecules to NO<sub>3</sub><sup>−</sup> at 1.3 V vs. Ag/AgCl for 15 min. VB12 was quantified stoichiometrically according to the oxidized nitrate anions, which were reduced with copper oxide nanocrystal decorated graphenic electrode. Cathodic polarization was conducted with a graphite rod electrode before nitrate reduction to eliminate the potential interferences. Under optimized experimental conditions, the presented approach gave a wide detection linear range of 0.15–7378 nmol L<sup>−1</sup> and the detection limit was 0.59 nmol L<sup>−1</sup>. The results for biological samples were comparable to those of the HPLC method. These results indicated that successively combined anodic and cathodic polarization enhanced the detection sensitivity and efficiency of the electrode towards VB12. The proposed electrode shows potential in terms of efficiency, reliability and accuracy for rapid determination of VB12 in biological samples. |
| first_indexed | 2024-03-09T04:46:26Z |
| format | Article |
| id | doaj.art-ec2db6af30654608844311bf18a2e3c9 |
| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-09T04:46:26Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Water |
| spelling | doaj.art-ec2db6af30654608844311bf18a2e3c92023-12-03T13:15:17ZengMDPI AGWater2073-44412021-06-011313179010.3390/w13131790Facile Detection of Vitamin B12 with Copper Oxide Nanocrystal Graphenic Composite ElectrodeChao Tian0Na Zhao1Xinyue Jiang2Duanji Wan3Yuqun Xie4School of Civil Engineering Architecture and Environment, Hubei University of Technology, Wuhan 430068, ChinaSchool of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan 430068, ChinaSchool of Civil Engineering Architecture and Environment, Hubei University of Technology, Wuhan 430068, ChinaSchool of Civil Engineering Architecture and Environment, Hubei University of Technology, Wuhan 430068, ChinaSchool of Bioengineering and Food Science, Hubei University of Technology, Wuhan 430068, ChinaVitamin B12 (VB12) is applied as the cofactors in various important enzymatic reactions and is involved in gene expression regulation mediated by B12-riboswitch and the VB12-dependent photoreceptor. Rapid detection VB12 concertation in a given environment may provide insights in the evaluation of micronutrient levels and the physiological and ecological performances of organisms under the relevant condition. This study demonstrating an amperometric approach to quantify the VB12 in biological samples without complicated sample pretreatment. The electrochemical oxidation step was conducted with a plain graphenic electrode to convert all nitrogen groups within the VB12 molecules to NO<sub>3</sub><sup>−</sup> at 1.3 V vs. Ag/AgCl for 15 min. VB12 was quantified stoichiometrically according to the oxidized nitrate anions, which were reduced with copper oxide nanocrystal decorated graphenic electrode. Cathodic polarization was conducted with a graphite rod electrode before nitrate reduction to eliminate the potential interferences. Under optimized experimental conditions, the presented approach gave a wide detection linear range of 0.15–7378 nmol L<sup>−1</sup> and the detection limit was 0.59 nmol L<sup>−1</sup>. The results for biological samples were comparable to those of the HPLC method. These results indicated that successively combined anodic and cathodic polarization enhanced the detection sensitivity and efficiency of the electrode towards VB12. The proposed electrode shows potential in terms of efficiency, reliability and accuracy for rapid determination of VB12 in biological samples.https://www.mdpi.com/2073-4441/13/13/1790copper oxideelectrochemical detectionGUITARelectrochemical oxidationvitamin B12 |
| spellingShingle | Chao Tian Na Zhao Xinyue Jiang Duanji Wan Yuqun Xie Facile Detection of Vitamin B12 with Copper Oxide Nanocrystal Graphenic Composite Electrode Water copper oxide electrochemical detection GUITAR electrochemical oxidation vitamin B12 |
| title | Facile Detection of Vitamin B12 with Copper Oxide Nanocrystal Graphenic Composite Electrode |
| title_full | Facile Detection of Vitamin B12 with Copper Oxide Nanocrystal Graphenic Composite Electrode |
| title_fullStr | Facile Detection of Vitamin B12 with Copper Oxide Nanocrystal Graphenic Composite Electrode |
| title_full_unstemmed | Facile Detection of Vitamin B12 with Copper Oxide Nanocrystal Graphenic Composite Electrode |
| title_short | Facile Detection of Vitamin B12 with Copper Oxide Nanocrystal Graphenic Composite Electrode |
| title_sort | facile detection of vitamin b12 with copper oxide nanocrystal graphenic composite electrode |
| topic | copper oxide electrochemical detection GUITAR electrochemical oxidation vitamin B12 |
| url | https://www.mdpi.com/2073-4441/13/13/1790 |
| work_keys_str_mv | AT chaotian faciledetectionofvitaminb12withcopperoxidenanocrystalgrapheniccompositeelectrode AT nazhao faciledetectionofvitaminb12withcopperoxidenanocrystalgrapheniccompositeelectrode AT xinyuejiang faciledetectionofvitaminb12withcopperoxidenanocrystalgrapheniccompositeelectrode AT duanjiwan faciledetectionofvitaminb12withcopperoxidenanocrystalgrapheniccompositeelectrode AT yuqunxie faciledetectionofvitaminb12withcopperoxidenanocrystalgrapheniccompositeelectrode |