Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum
We characterized a negative sense single-stranded RNA mycovirus, Fusarium oxysporum mymonavirus 1 (FoMyV1), isolated from the phytopathogenic fungus Fusarium oxysporum. The genome of FoMyV1 is 10,114 nt, including five open reading frames (ORFs1–5) that are non-overlapping and linearly arranged. The...
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.870204/full |
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| author | Jing Wang Chengjun Li Pengyu Song Rui Qiu Ruifang Song Xiaojie Li Yunxia Ni Hui Zhao Hongyan Liu Shujun Li |
| author_facet | Jing Wang Chengjun Li Pengyu Song Rui Qiu Ruifang Song Xiaojie Li Yunxia Ni Hui Zhao Hongyan Liu Shujun Li |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | We characterized a negative sense single-stranded RNA mycovirus, Fusarium oxysporum mymonavirus 1 (FoMyV1), isolated from the phytopathogenic fungus Fusarium oxysporum. The genome of FoMyV1 is 10,114 nt, including five open reading frames (ORFs1–5) that are non-overlapping and linearly arranged. The largest, ORF5, encodes a large polypeptide L containing a conserved regions corresponding to Mononegavirales RNA-dependent RNA polymerase and mRNA-capping enzyme region V; the putative functions of the remaining four ORFs are unknown. The L protein encoded by ORF5 shared a high amino acid identity of 65% with that of Hubei rhabdo-like virus 4, a mymonavirus that associated with arthropods. However, the L protein of FoMyV1 also showed amino acid similarity (27–36%) with proteins of mynonaviruses that infect the phytopathogenic fungi Sclerotinia sclerotiorum and Botrytis cineaea. Phylogenetic analysis based on L protein showed that FoMyV1 is clustered with the members of the genus Hubramonavirus in the family Mymonaviridae. Moreover, we found that FoMyV1 could successfully transfer by hyphal anastomosis to a virus-free strain. FoMyV1 reduced the vegetative growth and conidium production of its fungal host but did not alter its virulence. To the best of our knowledge, this is not only the first mymonavirus described in the species F. oxysporum, but also the first Hubramonavirus species found to infect a fungus. However, the incidence of FoMyV1 infections in the tested F. oxysporum strains was only 1%. |
| first_indexed | 2024-04-13T08:01:56Z |
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| language | English |
| last_indexed | 2024-04-13T08:01:56Z |
| publishDate | 2022-04-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-ec3343b303634125967313dbc5af57e22022-12-22T02:55:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-04-011310.3389/fmicb.2022.870204870204Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporumJing Wang0Chengjun Li1Pengyu Song2Rui Qiu3Ruifang Song4Xiaojie Li5Yunxia Ni6Hui Zhao7Hongyan Liu8Shujun Li9Key Laboratory for Green Preservation and Control of Tobacco Diseases and Pest in Huanghuai Growing Area, Institute of Tobacco, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaKey Laboratory for Green Preservation and Control of Tobacco Diseases and Pest in Huanghuai Growing Area, Institute of Tobacco, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaCollege of Biological Sciences, China Agricultural University, Beijing, ChinaKey Laboratory for Green Preservation and Control of Tobacco Diseases and Pest in Huanghuai Growing Area, Institute of Tobacco, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaTobacco Company of Henan Province, Zhengzhou, ChinaKey Laboratory for Green Preservation and Control of Tobacco Diseases and Pest in Huanghuai Growing Area, Institute of Tobacco, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaKey Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Key Laboratory of Crop Pest Control, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaKey Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Key Laboratory of Crop Pest Control, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaKey Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Key Laboratory of Crop Pest Control, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaKey Laboratory for Green Preservation and Control of Tobacco Diseases and Pest in Huanghuai Growing Area, Institute of Tobacco, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaWe characterized a negative sense single-stranded RNA mycovirus, Fusarium oxysporum mymonavirus 1 (FoMyV1), isolated from the phytopathogenic fungus Fusarium oxysporum. The genome of FoMyV1 is 10,114 nt, including five open reading frames (ORFs1–5) that are non-overlapping and linearly arranged. The largest, ORF5, encodes a large polypeptide L containing a conserved regions corresponding to Mononegavirales RNA-dependent RNA polymerase and mRNA-capping enzyme region V; the putative functions of the remaining four ORFs are unknown. The L protein encoded by ORF5 shared a high amino acid identity of 65% with that of Hubei rhabdo-like virus 4, a mymonavirus that associated with arthropods. However, the L protein of FoMyV1 also showed amino acid similarity (27–36%) with proteins of mynonaviruses that infect the phytopathogenic fungi Sclerotinia sclerotiorum and Botrytis cineaea. Phylogenetic analysis based on L protein showed that FoMyV1 is clustered with the members of the genus Hubramonavirus in the family Mymonaviridae. Moreover, we found that FoMyV1 could successfully transfer by hyphal anastomosis to a virus-free strain. FoMyV1 reduced the vegetative growth and conidium production of its fungal host but did not alter its virulence. To the best of our knowledge, this is not only the first mymonavirus described in the species F. oxysporum, but also the first Hubramonavirus species found to infect a fungus. However, the incidence of FoMyV1 infections in the tested F. oxysporum strains was only 1%.https://www.frontiersin.org/articles/10.3389/fmicb.2022.870204/fullmycovirusFusarium oxysporumMymonaviridaeHubramonavirusroot rots |
| spellingShingle | Jing Wang Chengjun Li Pengyu Song Rui Qiu Ruifang Song Xiaojie Li Yunxia Ni Hui Zhao Hongyan Liu Shujun Li Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum Frontiers in Microbiology mycovirus Fusarium oxysporum Mymonaviridae Hubramonavirus root rots |
| title | Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum |
| title_full | Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum |
| title_fullStr | Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum |
| title_full_unstemmed | Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum |
| title_short | Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum |
| title_sort | molecular and biological characterization of the first mymonavirus identified in fusarium oxysporum |
| topic | mycovirus Fusarium oxysporum Mymonaviridae Hubramonavirus root rots |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.870204/full |
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