Modified electrolyte leakage method for testing the oxidative stability of Pinus mugo Turra under ozone-induced stress
Electrolyte leakage (EL) is the method commonly used to test the cell membrane integrity of plants under stress conditions. The cells of the leaf may be damaged by ozone (O3) entering the intercellular space as an oxidative stress agent. The modified EL method was used to test the oxidative stabilit...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2023-01-01
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| Series: | Folia Oecologica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/foecol-2023-0001 |
| _version_ | 1797848543791153152 |
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| author | Bičárová Svetlana Lukasová Veronika Adamčíková Katarína Žatková Lucia Milovský Rastislav Shashikumar Anumol Pažitný Jozef Buchholcerová Anna Bilčík Dušan |
| author_facet | Bičárová Svetlana Lukasová Veronika Adamčíková Katarína Žatková Lucia Milovský Rastislav Shashikumar Anumol Pažitný Jozef Buchholcerová Anna Bilčík Dušan |
| author_sort | Bičárová Svetlana |
| collection | DOAJ |
| description | Electrolyte leakage (EL) is the method commonly used to test the cell membrane integrity of plants under stress conditions. The cells of the leaf may be damaged by ozone (O3) entering the intercellular space as an oxidative stress agent. The modified EL method was used to test the oxidative stability (OxS) of plant tissue against O3-induced oxidative stress. The modification includes simulation of the artificial oxidative stress by additional ozonation of plant samples in the laboratory chamber. This modified EL method was applied to Pinus mugo Turra needle samples collected in the subalpine zone of the High Tatra Mts (Western Carpathians), in the years 2019 and 2020. Changes in the chemical composition of samples after artificial ozonation were traced by gas chromatography/mass spectrometry (GC/MS) analysis. In addition, O3 uptake through open stomata was estimated by calculation of the modelled ozone dose (MO3D). We also conducted an inspection of visible injury (VIN) on the needle surface focused on the occurrence of O3-induced symptoms and biotic harmful agents. Regarding OxS results as well as VIN indices, P. mugo needles showed relatively low sensitivity to oxidative stress induced by O3. Therefore MO3D in a range between 14 and 16 mmol m−2 can be considered as O3 dose with minor phytotoxic effect on P. mugo growing in the mountains of central-eastern Europe. |
| first_indexed | 2024-04-09T18:29:13Z |
| format | Article |
| id | doaj.art-ec35a0fd0d394b7c9328bc94147658ec |
| institution | Directory Open Access Journal |
| issn | 1338-7014 |
| language | English |
| last_indexed | 2024-04-09T18:29:13Z |
| publishDate | 2023-01-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Folia Oecologica |
| spelling | doaj.art-ec35a0fd0d394b7c9328bc94147658ec2023-04-11T17:42:42ZengSciendoFolia Oecologica1338-70142023-01-0150111510.2478/foecol-2023-0001Modified electrolyte leakage method for testing the oxidative stability of Pinus mugo Turra under ozone-induced stressBičárová Svetlana0Lukasová Veronika1Adamčíková Katarína2Žatková Lucia3Milovský Rastislav4Shashikumar Anumol5Pažitný Jozef6Buchholcerová Anna7Bilčík Dušan8Earth Science Institute of the Slovak Academy of Sciences, Stará Lesná, 059 60 Tatranská Lomnica, SlovakiaEarth Science Institute of the Slovak Academy of Sciences, Stará Lesná, 059 60 Tatranská Lomnica, SlovakiaDepartment of Plant Pathology and Mycology, Institute of Forest Ecology of the Slovak Academy of Sciences, Akademická 2, 949 01Nitra, SlovakiaEarth Science Institute of the Slovak Academy of Sciences, Ďumbierska 1, 974 11Banská Bystrica, SlovakiaEarth Science Institute of the Slovak Academy of Sciences, Ďumbierska 1, 974 11Banská Bystrica, SlovakiaGroupe International d’Etudes des Forêts Sud-européennes G.I.E.F.S, 69, Avenue des Hespérides, 06300Nice, FranceDepartment of Plant Pathology and Mycology, Institute of Forest Ecology of the Slovak Academy of Sciences, Akademická 2, 949 01Nitra, SlovakiaEarth Science Institute of the Slovak Academy of Sciences, Stará Lesná, 059 60 Tatranská Lomnica, SlovakiaEarth Science Institute of the Slovak Academy of Sciences, Stará Lesná, 059 60 Tatranská Lomnica, SlovakiaElectrolyte leakage (EL) is the method commonly used to test the cell membrane integrity of plants under stress conditions. The cells of the leaf may be damaged by ozone (O3) entering the intercellular space as an oxidative stress agent. The modified EL method was used to test the oxidative stability (OxS) of plant tissue against O3-induced oxidative stress. The modification includes simulation of the artificial oxidative stress by additional ozonation of plant samples in the laboratory chamber. This modified EL method was applied to Pinus mugo Turra needle samples collected in the subalpine zone of the High Tatra Mts (Western Carpathians), in the years 2019 and 2020. Changes in the chemical composition of samples after artificial ozonation were traced by gas chromatography/mass spectrometry (GC/MS) analysis. In addition, O3 uptake through open stomata was estimated by calculation of the modelled ozone dose (MO3D). We also conducted an inspection of visible injury (VIN) on the needle surface focused on the occurrence of O3-induced symptoms and biotic harmful agents. Regarding OxS results as well as VIN indices, P. mugo needles showed relatively low sensitivity to oxidative stress induced by O3. Therefore MO3D in a range between 14 and 16 mmol m−2 can be considered as O3 dose with minor phytotoxic effect on P. mugo growing in the mountains of central-eastern Europe.https://doi.org/10.2478/foecol-2023-0001cell membrane integrityinjury indexmodelled ozone dosemountain environmentozonevisible injury |
| spellingShingle | Bičárová Svetlana Lukasová Veronika Adamčíková Katarína Žatková Lucia Milovský Rastislav Shashikumar Anumol Pažitný Jozef Buchholcerová Anna Bilčík Dušan Modified electrolyte leakage method for testing the oxidative stability of Pinus mugo Turra under ozone-induced stress Folia Oecologica cell membrane integrity injury index modelled ozone dose mountain environment ozone visible injury |
| title | Modified electrolyte leakage method for testing the oxidative stability of Pinus mugo Turra under ozone-induced stress |
| title_full | Modified electrolyte leakage method for testing the oxidative stability of Pinus mugo Turra under ozone-induced stress |
| title_fullStr | Modified electrolyte leakage method for testing the oxidative stability of Pinus mugo Turra under ozone-induced stress |
| title_full_unstemmed | Modified electrolyte leakage method for testing the oxidative stability of Pinus mugo Turra under ozone-induced stress |
| title_short | Modified electrolyte leakage method for testing the oxidative stability of Pinus mugo Turra under ozone-induced stress |
| title_sort | modified electrolyte leakage method for testing the oxidative stability of pinus mugo turra under ozone induced stress |
| topic | cell membrane integrity injury index modelled ozone dose mountain environment ozone visible injury |
| url | https://doi.org/10.2478/foecol-2023-0001 |
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