Transcriptome analysis and physiological changes in the leaves of two Bromus inermis L. genotypes in response to salt stress
Soil salinity is a major factor threatening the production of crops around the world. Smooth bromegrass (Bromus inermis L.) is a high-quality grass in northern and northwestern China. Currently, selecting and utilizing salt-tolerant genotypes is an important way to mitigate the detrimental effects o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1313113/full |
| _version_ | 1797391321120374784 |
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| author | Wenxue Song Xueqin Gao Xueqin Gao Huiping Li Shuxia Li Shuxia Li Jing Wang Xing Wang Tongrui Wang Yunong Ye Pengfei Hu Xiaohong Li Bingzhe Fu Bingzhe Fu Bingzhe Fu |
| author_facet | Wenxue Song Xueqin Gao Xueqin Gao Huiping Li Shuxia Li Shuxia Li Jing Wang Xing Wang Tongrui Wang Yunong Ye Pengfei Hu Xiaohong Li Bingzhe Fu Bingzhe Fu Bingzhe Fu |
| author_sort | Wenxue Song |
| collection | DOAJ |
| description | Soil salinity is a major factor threatening the production of crops around the world. Smooth bromegrass (Bromus inermis L.) is a high-quality grass in northern and northwestern China. Currently, selecting and utilizing salt-tolerant genotypes is an important way to mitigate the detrimental effects of salinity on crop productivity. In our research, salt-tolerant and salt-sensitive varieties were selected from 57 accessions based on a comprehensive evaluation of 22 relevant indexes, and their salt-tolerance physiological and molecular mechanisms were further analyzed. Results showed significant differences in salt tolerance between 57 genotypes, with Q25 and Q46 considered to be the most salt-tolerant and salt-sensitive accessions, respectively, compared to other varieties. Under saline conditions, the salt-tolerant genotype Q25 not only maintained significantly higher photosynthetic performance, leaf relative water content (RWC), and proline content but also exhibited obviously lower relative conductivity and malondialdehyde (MDA) content than the salt-sensitive Q46 (p < 0.05). The transcriptome sequencing indicated 15,128 differentially expressed genes (DEGs) in Q46, of which 7,885 were upregulated and 7,243 downregulated, and 12,658 DEGs in Q25, of which 6,059 were upregulated and 6,599 downregulated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the salt response differences between Q25 and Q46 were attributed to the variable expression of genes associated with plant hormone signal transduction and MAPK signaling pathways. Furthermore, a large number of candidate genes, related to salt tolerance, were detected, which involved transcription factors (zinc finger proteins) and accumulation of compatible osmolytes (glutathione S-transferases and pyrroline-5-carboxylate reductases), etc. This study offers an important view of the physiological and molecular regulatory mechanisms of salt tolerance in two smooth bromegrass genotypes and lays the foundation for further identification of key genes linked to salt tolerance. |
| first_indexed | 2024-03-08T23:31:12Z |
| format | Article |
| id | doaj.art-ec36e68d47fb4892a9ff62c9518ca581 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-08T23:31:12Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-ec36e68d47fb4892a9ff62c9518ca5812023-12-14T14:08:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-12-011410.3389/fpls.2023.13131131313113Transcriptome analysis and physiological changes in the leaves of two Bromus inermis L. genotypes in response to salt stressWenxue Song0Xueqin Gao1Xueqin Gao2Huiping Li3Shuxia Li4Shuxia Li5Jing Wang6Xing Wang7Tongrui Wang8Yunong Ye9Pengfei Hu10Xiaohong Li11Bingzhe Fu12Bingzhe Fu13Bingzhe Fu14College of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia, ChinaCollege of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia, ChinaNingxia Grassland and Animal Husbandry Engineering Technology Research Center, Yinchuan, Ningxia, ChinaCollege of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia, ChinaCollege of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia, ChinaNingxia Grassland and Animal Husbandry Engineering Technology Research Center, Yinchuan, Ningxia, ChinaCollege of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia, ChinaCollege of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia, ChinaCollege of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia, ChinaCollege of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia, ChinaCollege of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia, ChinaCollege of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia, ChinaCollege of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia, ChinaNingxia Grassland and Animal Husbandry Engineering Technology Research Center, Yinchuan, Ningxia, ChinaKey Laboratory for Model Innovation in Forage Production Efficiency, Ministry of Agriculture and Rural Affairs, Yinchuan, Ningxia, ChinaSoil salinity is a major factor threatening the production of crops around the world. Smooth bromegrass (Bromus inermis L.) is a high-quality grass in northern and northwestern China. Currently, selecting and utilizing salt-tolerant genotypes is an important way to mitigate the detrimental effects of salinity on crop productivity. In our research, salt-tolerant and salt-sensitive varieties were selected from 57 accessions based on a comprehensive evaluation of 22 relevant indexes, and their salt-tolerance physiological and molecular mechanisms were further analyzed. Results showed significant differences in salt tolerance between 57 genotypes, with Q25 and Q46 considered to be the most salt-tolerant and salt-sensitive accessions, respectively, compared to other varieties. Under saline conditions, the salt-tolerant genotype Q25 not only maintained significantly higher photosynthetic performance, leaf relative water content (RWC), and proline content but also exhibited obviously lower relative conductivity and malondialdehyde (MDA) content than the salt-sensitive Q46 (p < 0.05). The transcriptome sequencing indicated 15,128 differentially expressed genes (DEGs) in Q46, of which 7,885 were upregulated and 7,243 downregulated, and 12,658 DEGs in Q25, of which 6,059 were upregulated and 6,599 downregulated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the salt response differences between Q25 and Q46 were attributed to the variable expression of genes associated with plant hormone signal transduction and MAPK signaling pathways. Furthermore, a large number of candidate genes, related to salt tolerance, were detected, which involved transcription factors (zinc finger proteins) and accumulation of compatible osmolytes (glutathione S-transferases and pyrroline-5-carboxylate reductases), etc. This study offers an important view of the physiological and molecular regulatory mechanisms of salt tolerance in two smooth bromegrass genotypes and lays the foundation for further identification of key genes linked to salt tolerance.https://www.frontiersin.org/articles/10.3389/fpls.2023.1313113/fullBromus inermis L.salt stressaccessions evaluationphysiological analysistranscriptome analysis |
| spellingShingle | Wenxue Song Xueqin Gao Xueqin Gao Huiping Li Shuxia Li Shuxia Li Jing Wang Xing Wang Tongrui Wang Yunong Ye Pengfei Hu Xiaohong Li Bingzhe Fu Bingzhe Fu Bingzhe Fu Transcriptome analysis and physiological changes in the leaves of two Bromus inermis L. genotypes in response to salt stress Frontiers in Plant Science Bromus inermis L. salt stress accessions evaluation physiological analysis transcriptome analysis |
| title | Transcriptome analysis and physiological changes in the leaves of two Bromus inermis L. genotypes in response to salt stress |
| title_full | Transcriptome analysis and physiological changes in the leaves of two Bromus inermis L. genotypes in response to salt stress |
| title_fullStr | Transcriptome analysis and physiological changes in the leaves of two Bromus inermis L. genotypes in response to salt stress |
| title_full_unstemmed | Transcriptome analysis and physiological changes in the leaves of two Bromus inermis L. genotypes in response to salt stress |
| title_short | Transcriptome analysis and physiological changes in the leaves of two Bromus inermis L. genotypes in response to salt stress |
| title_sort | transcriptome analysis and physiological changes in the leaves of two bromus inermis l genotypes in response to salt stress |
| topic | Bromus inermis L. salt stress accessions evaluation physiological analysis transcriptome analysis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1313113/full |
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