An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue
Objective(s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apo...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2018-05-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_10541_c17c3d587608094e1fbddecb80ba8ef2.pdf |
| _version_ | 1818927493066784768 |
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| author | Elif Iplik Baris Ertugrul İlknur Kozanoglu Yusuf Baran Bedia Cakmakoglu |
| author_facet | Elif Iplik Baris Ertugrul İlknur Kozanoglu Yusuf Baran Bedia Cakmakoglu |
| author_sort | Elif Iplik |
| collection | DOAJ |
| description | Objective(s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer.Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-29, HCT-116, RKO and healthy cell line MRC5 were cultured by different seeding procedure. After cell viability assay, changes in caspase 3 enzyme activity and the level of phosphatidylserine were measured.Results: For cell viability assay, a 48 hr incubation period was chosen to seed all cells together. There was a 1.36-fold decrease in caspase 3 enzyme activity by co-treatment of RKO and MSCs in addition to 2.02-fold decrease in HT-29 and MSCs co-treatment, and 1.103-fold increase in HCT-116 and MSCs. The results demonstrated that HCT-116 led to the highest rate of apoptotic cell death (7.5%) compared with other cells.Conclusion: We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity. |
| first_indexed | 2024-12-20T03:13:53Z |
| format | Article |
| id | doaj.art-ec3c3e0a27cd49f48f198a74f6dbc8e4 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-20T03:13:53Z |
| publishDate | 2018-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-ec3c3e0a27cd49f48f198a74f6dbc8e42022-12-21T19:55:24ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742018-05-0121546546810.22038/ijbms.2018.26152.642010541An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissueElif Iplik0Baris Ertugrul1İlknur Kozanoglu2Yusuf Baran3Bedia Cakmakoglu4Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Istanbul Yeni Yuzyil University, Istanbul, TurkeyDepartment of Molecular Medicine, Aziz Sancar Institute for Experimental Medicine, Istanbul University, Istanbul, TurkeyDepartment of Hematology, Baskent University, Adana, TurkeyFaculty of Life and Natural Sciences, Abdullah Gul University, Kayseri, Turkey|Department of Molecular Biology and Genetics, Izmir Institute of Technology, Urla, Izmir, TurkeyDepartment of Molecular Medicine, Aziz Sancar Institute for Experimental Medicine, Istanbul University, Istanbul, TurkeyObjective(s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer.Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-29, HCT-116, RKO and healthy cell line MRC5 were cultured by different seeding procedure. After cell viability assay, changes in caspase 3 enzyme activity and the level of phosphatidylserine were measured.Results: For cell viability assay, a 48 hr incubation period was chosen to seed all cells together. There was a 1.36-fold decrease in caspase 3 enzyme activity by co-treatment of RKO and MSCs in addition to 2.02-fold decrease in HT-29 and MSCs co-treatment, and 1.103-fold increase in HCT-116 and MSCs. The results demonstrated that HCT-116 led to the highest rate of apoptotic cell death (7.5%) compared with other cells.Conclusion: We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity.http://ijbms.mums.ac.ir/article_10541_c17c3d587608094e1fbddecb80ba8ef2.pdfApoptosisColon cancerStem cellsCell death |
| spellingShingle | Elif Iplik Baris Ertugrul İlknur Kozanoglu Yusuf Baran Bedia Cakmakoglu An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue Iranian Journal of Basic Medical Sciences Apoptosis Colon cancer Stem cells Cell death |
| title | An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue |
| title_full | An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue |
| title_fullStr | An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue |
| title_full_unstemmed | An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue |
| title_short | An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue |
| title_sort | answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue |
| topic | Apoptosis Colon cancer Stem cells Cell death |
| url | http://ijbms.mums.ac.ir/article_10541_c17c3d587608094e1fbddecb80ba8ef2.pdf |
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