Protective Effect of Resveratrol Improves Systemic Inflammation Responses in LPS-Injected Lambs
Highly intensive livestock production often causes immune stress to animals, which makes them more susceptible to infections. The aim of this study was to examine whether resveratrol (Res) alleviates inflammation in lambs. In Experiment 1, 16 male lambs were injected with lipopolysaccharides (LPS) a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/9/11/872 |
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| author | Yanping Liang Jianwei Zhou Kaixi Ji Hu Liu Allan Degen Manjun Zhai Dan Jiao Junqiang Guo Zongsheng Zhao Guo Yang |
| author_facet | Yanping Liang Jianwei Zhou Kaixi Ji Hu Liu Allan Degen Manjun Zhai Dan Jiao Junqiang Guo Zongsheng Zhao Guo Yang |
| author_sort | Yanping Liang |
| collection | DOAJ |
| description | Highly intensive livestock production often causes immune stress to animals, which makes them more susceptible to infections. The aim of this study was to examine whether resveratrol (Res) alleviates inflammation in lambs. In Experiment 1, 16 male lambs were injected with lipopolysaccharides (LPS) at an initial dose of 0.25, 1.25, and 2.5 μg/kg body weight (BW) for 9 days. Average daily gain and blood parameters were measured and clinical symptoms were recorded. In Experiment 2, 20 male lambs were injected intravenously with LPS (0 mg/kg) + Res (0 mg), LPS (2.5 μg /kg) + Res (0 mg, 82.5 mg, 165 mg, 330 mg), 4 h after LPS injection. Jugular blood was collected from each lamb to determine white blood cell (WBC) counts and the expression of inflammatory genes. In Experiment 1, all LPS-treated lambs showed clinical signs of sickness including rhinorrhea, lethargy, and shivering, and systemic inflammatory responses of increased inflammatory genes levels and cortisol concentration. The lambs had increased respiratory and heart rates and rectal temperature and decreased average daily gain and feed intake. In Experiment 2, resveratrol significantly reduced WBCs and the expression levels of several genes associated with inflammation response (<i>TLR4</i>, <i>NF-κB</i>, <i>c-jun</i>) and inhibited the signaling cascades of <i>NF-κB</i> and MAPKs by down-regulating the expression levels of inflammatory cytokines (<i>IL-1β</i>, <i>IL-4</i>, <i>IL-6</i>, <i>TNF-α</i>, <i>IFN-γ</i>) induced by LPS. Resveratrol attenuated the LPS-evoked inflammatory responses in lambs by suppressing expression levels of inflammatory cytokines, and blocking <i>NF-κB</i> and MAPK signaling pathways. |
| first_indexed | 2024-12-16T08:14:59Z |
| format | Article |
| id | doaj.art-ec4327391a2d411490ea712cea408f70 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-12-16T08:14:59Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ec4327391a2d411490ea712cea408f702022-12-21T22:38:17ZengMDPI AGAnimals2076-26152019-10-0191187210.3390/ani9110872ani9110872Protective Effect of Resveratrol Improves Systemic Inflammation Responses in LPS-Injected LambsYanping Liang0Jianwei Zhou1Kaixi Ji2Hu Liu3Allan Degen4Manjun Zhai5Dan Jiao6Junqiang Guo7Zongsheng Zhao8Guo Yang9College of Animal Science and Technology, Shihezi University, Shihezi 832003, ChinaNorthwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaNorthwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaNorthwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaDesert Animal Adaptations and Husbandry, Wyler Department of Dryland Agriculture, Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Beer Sheva 8410500 IsraelCollege of Animal Science and Technology, Shihezi University, Shihezi 832003, ChinaNorthwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaNorthwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi 832003, ChinaNorthwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaHighly intensive livestock production often causes immune stress to animals, which makes them more susceptible to infections. The aim of this study was to examine whether resveratrol (Res) alleviates inflammation in lambs. In Experiment 1, 16 male lambs were injected with lipopolysaccharides (LPS) at an initial dose of 0.25, 1.25, and 2.5 μg/kg body weight (BW) for 9 days. Average daily gain and blood parameters were measured and clinical symptoms were recorded. In Experiment 2, 20 male lambs were injected intravenously with LPS (0 mg/kg) + Res (0 mg), LPS (2.5 μg /kg) + Res (0 mg, 82.5 mg, 165 mg, 330 mg), 4 h after LPS injection. Jugular blood was collected from each lamb to determine white blood cell (WBC) counts and the expression of inflammatory genes. In Experiment 1, all LPS-treated lambs showed clinical signs of sickness including rhinorrhea, lethargy, and shivering, and systemic inflammatory responses of increased inflammatory genes levels and cortisol concentration. The lambs had increased respiratory and heart rates and rectal temperature and decreased average daily gain and feed intake. In Experiment 2, resveratrol significantly reduced WBCs and the expression levels of several genes associated with inflammation response (<i>TLR4</i>, <i>NF-κB</i>, <i>c-jun</i>) and inhibited the signaling cascades of <i>NF-κB</i> and MAPKs by down-regulating the expression levels of inflammatory cytokines (<i>IL-1β</i>, <i>IL-4</i>, <i>IL-6</i>, <i>TNF-α</i>, <i>IFN-γ</i>) induced by LPS. Resveratrol attenuated the LPS-evoked inflammatory responses in lambs by suppressing expression levels of inflammatory cytokines, and blocking <i>NF-κB</i> and MAPK signaling pathways.https://www.mdpi.com/2076-2615/9/11/872resveratrollipopolysaccharide (lps)inflammation responsescytokineslamb |
| spellingShingle | Yanping Liang Jianwei Zhou Kaixi Ji Hu Liu Allan Degen Manjun Zhai Dan Jiao Junqiang Guo Zongsheng Zhao Guo Yang Protective Effect of Resveratrol Improves Systemic Inflammation Responses in LPS-Injected Lambs Animals resveratrol lipopolysaccharide (lps) inflammation responses cytokines lamb |
| title | Protective Effect of Resveratrol Improves Systemic Inflammation Responses in LPS-Injected Lambs |
| title_full | Protective Effect of Resveratrol Improves Systemic Inflammation Responses in LPS-Injected Lambs |
| title_fullStr | Protective Effect of Resveratrol Improves Systemic Inflammation Responses in LPS-Injected Lambs |
| title_full_unstemmed | Protective Effect of Resveratrol Improves Systemic Inflammation Responses in LPS-Injected Lambs |
| title_short | Protective Effect of Resveratrol Improves Systemic Inflammation Responses in LPS-Injected Lambs |
| title_sort | protective effect of resveratrol improves systemic inflammation responses in lps injected lambs |
| topic | resveratrol lipopolysaccharide (lps) inflammation responses cytokines lamb |
| url | https://www.mdpi.com/2076-2615/9/11/872 |
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