Association of Mitochondrial Pyruvate Carrier with the Clinical and Histological Features in Lupus Nephritis
Huanhuan Zhu, Chen Chen, Luhan Geng, Qing Li, Chengning Zhang, Lin Wu, Bo Zhang, Suyan Duan,* Changying Xing,* Yanggang Yuan* Department of Nephrology, the First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, People’s Republic of China...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2024-01-01
|
| Series: | International Journal of Nephrology and Renovascular Disease |
| Subjects: | |
| Online Access: | https://www.dovepress.com/association-of-mitochondrial-pyruvate-carrier-with-the-clinical-and-hi-peer-reviewed-fulltext-article-IJNRD |
| _version_ | 1827377018587578368 |
|---|---|
| author | Zhu H Chen C Geng L Li Q Zhang C Wu L Zhang B Duan S Xing C Yuan Y |
| author_facet | Zhu H Chen C Geng L Li Q Zhang C Wu L Zhang B Duan S Xing C Yuan Y |
| author_sort | Zhu H |
| collection | DOAJ |
| description | Huanhuan Zhu, Chen Chen, Luhan Geng, Qing Li, Chengning Zhang, Lin Wu, Bo Zhang, Suyan Duan,* Changying Xing,* Yanggang Yuan* Department of Nephrology, the First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yanggang Yuan, Department of Nephrology, the First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu Province, 210029, People’s Republic of China, Tel/Fax +86-25-6813-6462, Email ygyuan@njmu.edu.cnBackground: Mounting evidence suggests that mitochondrial dysfunction contributes to lupus nephritis (LN) pathogenesis. Mitochondrial pyruvate carrier 1 (MPC1) and mitochondrial pyruvate carrier 2 (MPC2) mediating pyruvate transport from the cytoplasm to the mitochondrial matrix, determines the cell survival and cellular energy supply. Here, we aimed to investigate the association of mitochondrial pyruvate carrier expression with the clinical and histological features in LN.Methods: Patients with biopsy-proven proliferative LN (class III and class IV, n=18) and membranous LN (class V, n=18) were included. Expression of MPC1 and MPC2 were examined by immunohistochemistry. MPC protein levels in the two groups were evaluated by the Student’s t-test. Correlation analysis between MPC levels and clinicopathological features was performed by Spearman’s rank correlation.Results: Both MPC1 and MPC2 were exclusively expressed in renal tubules of enrolled LN. Significantly lower MPC1 and MPC2 were observed in patients with proliferative LN compared to membranous LN. In addition, the MPC1 and MPC2 were negatively correlated with SLEDAI-2K score, renal function, and renal pathology activity index.Conclusion: Both MPC1 and MPC2 were localized in renal tubules, and decreased MPC content was more pronounced in proliferative LN than membranous LN. MPC levels were significantly correlated with renal functions and renal pathology activity.Keywords: lupus nephritis, mitochondrial pyruvate carrier, proliferative, membranous, tubulointerstitial lesions |
| first_indexed | 2024-03-08T12:32:52Z |
| format | Article |
| id | doaj.art-ec4483dded7847acbc8ad3d0cbcc7540 |
| institution | Directory Open Access Journal |
| issn | 1178-7058 |
| language | English |
| last_indexed | 2024-03-08T12:32:52Z |
| publishDate | 2024-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nephrology and Renovascular Disease |
| spelling | doaj.art-ec4483dded7847acbc8ad3d0cbcc75402024-01-21T18:27:54ZengDove Medical PressInternational Journal of Nephrology and Renovascular Disease1178-70582024-01-01Volume 17293889819Association of Mitochondrial Pyruvate Carrier with the Clinical and Histological Features in Lupus NephritisZhu HChen CGeng LLi QZhang CWu LZhang BDuan SXing CYuan YHuanhuan Zhu, Chen Chen, Luhan Geng, Qing Li, Chengning Zhang, Lin Wu, Bo Zhang, Suyan Duan,* Changying Xing,* Yanggang Yuan* Department of Nephrology, the First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yanggang Yuan, Department of Nephrology, the First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu Province, 210029, People’s Republic of China, Tel/Fax +86-25-6813-6462, Email ygyuan@njmu.edu.cnBackground: Mounting evidence suggests that mitochondrial dysfunction contributes to lupus nephritis (LN) pathogenesis. Mitochondrial pyruvate carrier 1 (MPC1) and mitochondrial pyruvate carrier 2 (MPC2) mediating pyruvate transport from the cytoplasm to the mitochondrial matrix, determines the cell survival and cellular energy supply. Here, we aimed to investigate the association of mitochondrial pyruvate carrier expression with the clinical and histological features in LN.Methods: Patients with biopsy-proven proliferative LN (class III and class IV, n=18) and membranous LN (class V, n=18) were included. Expression of MPC1 and MPC2 were examined by immunohistochemistry. MPC protein levels in the two groups were evaluated by the Student’s t-test. Correlation analysis between MPC levels and clinicopathological features was performed by Spearman’s rank correlation.Results: Both MPC1 and MPC2 were exclusively expressed in renal tubules of enrolled LN. Significantly lower MPC1 and MPC2 were observed in patients with proliferative LN compared to membranous LN. In addition, the MPC1 and MPC2 were negatively correlated with SLEDAI-2K score, renal function, and renal pathology activity index.Conclusion: Both MPC1 and MPC2 were localized in renal tubules, and decreased MPC content was more pronounced in proliferative LN than membranous LN. MPC levels were significantly correlated with renal functions and renal pathology activity.Keywords: lupus nephritis, mitochondrial pyruvate carrier, proliferative, membranous, tubulointerstitial lesionshttps://www.dovepress.com/association-of-mitochondrial-pyruvate-carrier-with-the-clinical-and-hi-peer-reviewed-fulltext-article-IJNRDlupus nephritismitochondrial pyruvate carrierproliferativemembranoustubulointerstitial lesions |
| spellingShingle | Zhu H Chen C Geng L Li Q Zhang C Wu L Zhang B Duan S Xing C Yuan Y Association of Mitochondrial Pyruvate Carrier with the Clinical and Histological Features in Lupus Nephritis International Journal of Nephrology and Renovascular Disease lupus nephritis mitochondrial pyruvate carrier proliferative membranous tubulointerstitial lesions |
| title | Association of Mitochondrial Pyruvate Carrier with the Clinical and Histological Features in Lupus Nephritis |
| title_full | Association of Mitochondrial Pyruvate Carrier with the Clinical and Histological Features in Lupus Nephritis |
| title_fullStr | Association of Mitochondrial Pyruvate Carrier with the Clinical and Histological Features in Lupus Nephritis |
| title_full_unstemmed | Association of Mitochondrial Pyruvate Carrier with the Clinical and Histological Features in Lupus Nephritis |
| title_short | Association of Mitochondrial Pyruvate Carrier with the Clinical and Histological Features in Lupus Nephritis |
| title_sort | association of mitochondrial pyruvate carrier with the clinical and histological features in lupus nephritis |
| topic | lupus nephritis mitochondrial pyruvate carrier proliferative membranous tubulointerstitial lesions |
| url | https://www.dovepress.com/association-of-mitochondrial-pyruvate-carrier-with-the-clinical-and-hi-peer-reviewed-fulltext-article-IJNRD |
| work_keys_str_mv | AT zhuh associationofmitochondrialpyruvatecarrierwiththeclinicalandhistologicalfeaturesinlupusnephritis AT chenc associationofmitochondrialpyruvatecarrierwiththeclinicalandhistologicalfeaturesinlupusnephritis AT gengl associationofmitochondrialpyruvatecarrierwiththeclinicalandhistologicalfeaturesinlupusnephritis AT liq associationofmitochondrialpyruvatecarrierwiththeclinicalandhistologicalfeaturesinlupusnephritis AT zhangc associationofmitochondrialpyruvatecarrierwiththeclinicalandhistologicalfeaturesinlupusnephritis AT wul associationofmitochondrialpyruvatecarrierwiththeclinicalandhistologicalfeaturesinlupusnephritis AT zhangb associationofmitochondrialpyruvatecarrierwiththeclinicalandhistologicalfeaturesinlupusnephritis AT duans associationofmitochondrialpyruvatecarrierwiththeclinicalandhistologicalfeaturesinlupusnephritis AT xingc associationofmitochondrialpyruvatecarrierwiththeclinicalandhistologicalfeaturesinlupusnephritis AT yuany associationofmitochondrialpyruvatecarrierwiththeclinicalandhistologicalfeaturesinlupusnephritis |